The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability

DNA-protein crosslinks (DPCs) are a specific type of DNA lesion in which proteins are covalently attached to DNA. Unrepaired DPCs lead to genomic instability, cancer, neurodegeneration, and accelerated aging. DPC proteolysis was recently identified as a specialized pathway for DPC repair. The DNA-de...

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Vydané v:	Cell reports (Cambridge) Ročník 37; číslo 10; s. 110080
Hlavní autori:	Ruggiano, Annamaria, Vaz, Bruno, Kilgas, Susan, Popović, Marta, Rodriguez-Berriguete, Gonzalo, Singh, Abhay N., Higgins, Geoff S., Kiltie, Anne E., Ramadan, Kristijan
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	United States Elsevier Inc 07.12.2021 Cell Press
Predmet:	BRCA deficiency DNA Breaks, Double-Stranded DNA Damage DNA Repair DNA Replication DNA, Neoplasm - biosynthesis DNA, Neoplasm - genetics DNA, Neoplasm - metabolism DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism DNA-protein crosslink repair Female formaldehyde toxicity genome stability Genomic Instability HEK293 Cells HeLa Cells Homologous Recombination Humans Male Proteolysis SPRTN protease SUMO Sumoylation Synthetic Lethal Mutations synthetic lethality ubiquitin genome stability DNA replication SUMO homologous recombination DNA-protein crosslink repair SPRTN protease synthetic lethality formaldehyde toxicity BRCA deficiency ubiquitin
ISSN:	2211-1247, 2211-1247
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Shrnutí:	DNA-protein crosslinks (DPCs) are a specific type of DNA lesion in which proteins are covalently attached to DNA. Unrepaired DPCs lead to genomic instability, cancer, neurodegeneration, and accelerated aging. DPC proteolysis was recently identified as a specialized pathway for DPC repair. The DNA-dependent protease SPRTN and the 26S proteasome emerged as two independent proteolytic systems. DPCs are also repaired by homologous recombination (HR), a canonical DNA repair pathway. While studying the cellular response to DPC formation, we identify ubiquitylation and SUMOylation as two major signaling events in DNA replication-coupled DPC repair. DPC ubiquitylation recruits SPRTN to repair sites, promoting DPC removal. DPC SUMOylation prevents DNA double-strand break formation, HR activation, and potentially deleterious genomic rearrangements. In this way, SUMOylation channels DPC repair toward SPRTN proteolysis, which is a safer pathway choice for DPC repair and prevention of genomic instability. [Display omitted] •SUMOylation and ubiquitylation enable DPC repair during DNA replication•Resolution of ubiquitylated and SUMOylated DPCs requires the protease SPRTN•Inactivation of SUMOylation after DPC formation activates homologous recombination•DPC SUMOylation and SPRTN prevent recombination-dependent genomic instability Ruggiano et al. show that ubiquitylation and SUMOylation of DNA-protein crosslinks is linked to repair by SPRTN during DNA replication. Blocking SUMOylation causes double-strand breaks and switches the repair pathway to homologous recombination, potentially causing chromosomal rearrangements and genomic instability. Inactivation of DPC repair causes synthetic lethality in recombination-defective cells.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Present address: The Rowett Institute, University of Aberdeen, Foresterhill Road, Aberdeen AB25 2ZD, UK These authors contributed equally Lead contact
ISSN:	2211-1247 2211-1247
DOI:	10.1016/j.celrep.2021.110080