Tumor necrosis factor reduces Plasmodium falciparum growth and activates calcium signaling in human malaria parasites

Plasmodium has a complex biology including the ability to interact with host signals modulating their function through cellular machinery. Tumor necrosis factor (TNF) elicits diverse cellular responses including effects in malarial pathology and increased infected erythrocyte cytoadherence. As TNF l...

Celý popis

Uloženo v:

Podrobná bibliografie
Vydáno v:	Biochimica et biophysica acta Ročník 1860; číslo 7; s. 1489 - 1497
Hlavní autoři:	Cruz, Laura N., Wu, Yang, Ulrich, Henning, Craig, Alister G., Garcia, Célia R.S.
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Netherlands Elsevier B.V 01.07.2016
Témata:	agglutinins agonists antigens Antimalarials - pharmacology calcium Calcium signaling Calcium Signaling - drug effects cell adhesion Cell Adhesion - drug effects cell proliferation Cells, Cultured confocal microscopy cyclic AMP Cytoadhesion Dose-Response Relationship, Drug Endothelial Cells - drug effects Endothelial Cells - metabolism Endothelial Cells - parasitology erythrocytes Erythrocytes - parasitology ethylene glycol tetraacetic acid genes Host-Parasite Interactions Humans Malaria parasitemia parasites Plasmodium falciparum Plasmodium falciparum - drug effects Plasmodium falciparum - growth & development Plasmodium falciparum - immunology Plasmodium falciparum - metabolism Proliferating Cell Nuclear Antigen - metabolism Proliferating cell nuclear antigen-1 Protozoan Proteins - metabolism quantitative polymerase chain reaction sodium Time Factors Tumor necrosis factor Tumor Necrosis Factor-alpha - pharmacology tumor necrosis factors wheat germ Calcium signaling Proliferating cell nuclear antigen-1 Plasmodium falciparum Malaria Tumor necrosis factor Cytoadhesion
ISSN:	0304-4165, 0006-3002, 1872-8006
On-line přístup:	Získat plný text
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!

Popis
Shrnutí:	Plasmodium has a complex biology including the ability to interact with host signals modulating their function through cellular machinery. Tumor necrosis factor (TNF) elicits diverse cellular responses including effects in malarial pathology and increased infected erythrocyte cytoadherence. As TNF levels are raised during Plasmodium falciparum infection we have investigated whether it has an effect on the parasite asexual stage. Flow cytometry, spectrofluorimetric determinations, confocal microscopy and PCR real time quantifications were employed for characterizing TNF induced effects and membrane integrity verified by wheat germ agglutinin staining. TNF is able to decrease intracellular parasitemia, involving calcium as a second messenger of the pathway. Parasites incubated for 48h with TNF showed reduced erythrocyte invasion. Thus, TNF induced rises in intracellular calcium concentration, which were blocked by prior addition of the purinergic receptor agonists KN62 and A438079, or interfering with intra- or extracellular calcium release by thapsigargin or EGTA (ethylene glycol tetraacetic acid). Importantly, expression of PfPCNA1 which encodes the Plasmodium falciparum Proliferating-Cell Nuclear Antigen 1, decreased after P. falciparum treatment of TNF (tumor necrosis factor) or 6-Bnz cAMP (N6-benzoyladenosine-3′,5′-cyclic monophosphate sodium salt). This is potentially interesting data showing the relevance of calcium in downregulating a gene involved in cellular proliferation, triggered by TNF. The data show that Plasmodium may subvert the immunological system and use TNF for the control of its proliferation within the vertebrate host. •TNF is able to decrease parasitemia in P. falciparum‐infected RBCs.•TNF induced rises in intracellular calcium concentration, which were blocked by the purinergic receptor agonists KN62 and A438079.•Interfering with intra‐ or extracellular calcium release by thapsigargin or EGTA also block TNF‐induce calcium release in P. falciparum.•Expression of the P. falciparum Proliferating‐Cell Nuclear Antigen 1 (PfPCNA1) decreased after P. falciparum treatment with TNF or 6‐Bnz cAMP.•The data show that Plasmodium may subvert the immunological system and use TNF for the control of its proliferation within the vertebrate host.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0304-4165 0006-3002 1872-8006
DOI:	10.1016/j.bbagen.2016.04.003