Establishment of a human iPSC line (MUSIi007-A) from peripheral blood of normal individual using Sendai viral vectors

Human induced pluripotent stem cell (iPSC) line was generated from peripheral blood mononuclear cells (PBMNCs) isolated from a 26-year-old healthy subject to use as a control group for the iPSC line carrying compound heterozygote for mutation in KLF1 gene. The cells were reprogrammed using integrati...

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Published in:Stem cell research Vol. 32; pp. 43 - 46
Main Authors: Potirat, Ponthip, Wattanapanitch, Methichit, Kheolamai, Pakpoom, Issaragrisil, Surapol
Format: Journal Article
Language:English
Published: England Elsevier B.V 01.10.2018
Elsevier
Subjects:
Cell Differentiation - genetics
Cell Differentiation - physiology
Cell Line
Cells, Cultured
Cellular Reprogramming - genetics
Cellular Reprogramming - physiology
Genetic Vectors - genetics
Humans
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Karyotype
Leukocytes, Mononuclear - cytology
Leukocytes, Mononuclear - metabolism
Sendai virus - genetics
ISSN:1873-5061, 1876-7753, 1876-7753
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Abstract Human induced pluripotent stem cell (iPSC) line was generated from peripheral blood mononuclear cells (PBMNCs) isolated from a 26-year-old healthy subject to use as a control group for the iPSC line carrying compound heterozygote for mutation in KLF1 gene. The cells were reprogrammed using integration-free method, Sendai viral (SeV) vectors containing KOS, hc-MYC and hKLF4. The established iPSC line (MUSIi007-A) exhibited a normal karyotype, expressed pluripotent markers and displayed in vitro and in vivo differentiation potential into cells of three embryonic germ layers. Resource tableUnlabelled TableUnique stem cell line identifierMUSIi007-AAlternative name(s) of stem cell lineSeV-iPSCInstitutionSiriraj Center of Excellence for Stem Cell Research,Faculty of Medicine Siriraj Hospital, Mahidol UniversityContact information of distributorPonthip Potirat, ponthip.pot@gmail.comSurapol Issaragrisil, surapolsi@gmail.comType of cell lineiPSCsOriginHumanAdditional origin infoAge: 26-year-oldSex: MaleEthnicity: ThaiCell sourcePeripheral blood mononuclear cellsClonalityClonalMethod of reprogrammingIntegration-free Sendai viral vectorsGenetic modificationN/AType of modificationN/AAssociated diseaseN/AGene/locusN/AMethod of modificationN/AName of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock dateDecember 2014Cell line repository/bankN/AEthical approvalSiriraj Institutional Review Board (SiRB), no. Si248/2011, Faculty of Medicine Siriraj Hospital, Mahidol University, Thailand.
AbstractList Human induced pluripotent stem cell (iPSC) line was generated from peripheral blood mononuclear cells (PBMNCs) isolated from a 26-year-old healthy subject to use as a control group for the iPSC line carrying compound heterozygote for mutation in KLF1 gene. The cells were reprogrammed using integration-free method, Sendai viral (SeV) vectors containing KOS, hc-MYC and hKLF4. The established iPSC line (MUSIi007-A) exhibited a normal karyotype, expressed pluripotent markers and displayed in vitro and in vivo differentiation potential into cells of three embryonic germ layers. Resource table.
Human induced pluripotent stem cell (iPSC) line was generated from peripheral blood mononuclear cells (PBMNCs) isolated from a 26-year-old healthy subject to use as a control group for the iPSC line carrying compound heterozygote for mutation in KLF1 gene. The cells were reprogrammed using integration-free method, Sendai viral (SeV) vectors containing KOS, hc-MYC and hKLF4. The established iPSC line (MUSIi007-A) exhibited a normal karyotype, expressed pluripotent markers and displayed in vitro and in vivo differentiation potential into cells of three embryonic germ layers. Resource tableUnlabelled TableUnique stem cell line identifierMUSIi007-AAlternative name(s) of stem cell lineSeV-iPSCInstitutionSiriraj Center of Excellence for Stem Cell Research,Faculty of Medicine Siriraj Hospital, Mahidol UniversityContact information of distributorPonthip Potirat, ponthip.pot@gmail.comSurapol Issaragrisil, surapolsi@gmail.comType of cell lineiPSCsOriginHumanAdditional origin infoAge: 26-year-oldSex: MaleEthnicity: ThaiCell sourcePeripheral blood mononuclear cellsClonalityClonalMethod of reprogrammingIntegration-free Sendai viral vectorsGenetic modificationN/AType of modificationN/AAssociated diseaseN/AGene/locusN/AMethod of modificationN/AName of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock dateDecember 2014Cell line repository/bankN/AEthical approvalSiriraj Institutional Review Board (SiRB), no. Si248/2011, Faculty of Medicine Siriraj Hospital, Mahidol University, Thailand.
Human induced pluripotent stem cell (iPSC) line was generated from peripheral blood mononuclear cells (PBMNCs) isolated from a 26-year-old healthy subject to use as a control group for the iPSC line carrying compound heterozygote for mutation in KLF1 gene. The cells were reprogrammed using integration-free method, Sendai viral (SeV) vectors containing KOS, hc-MYC and hKLF4. The established iPSC line (MUSIi007-A) exhibited a normal karyotype, expressed pluripotent markers and displayed in vitro and in vivo differentiation potential into cells of three embryonic germ layers. Resource table.Human induced pluripotent stem cell (iPSC) line was generated from peripheral blood mononuclear cells (PBMNCs) isolated from a 26-year-old healthy subject to use as a control group for the iPSC line carrying compound heterozygote for mutation in KLF1 gene. The cells were reprogrammed using integration-free method, Sendai viral (SeV) vectors containing KOS, hc-MYC and hKLF4. The established iPSC line (MUSIi007-A) exhibited a normal karyotype, expressed pluripotent markers and displayed in vitro and in vivo differentiation potential into cells of three embryonic germ layers. Resource table.
Human induced pluripotent stem cell (iPSC) line was generated from peripheral blood mononuclear cells (PBMNCs) isolated from a 26-year-old healthy subject to use as a control group for the iPSC line carrying compound heterozygote for mutation in KLF1 gene. The cells were reprogrammed using integration-free method, Sendai viral (SeV) vectors containing KOS, hc-MYC and hKLF4. The established iPSC line (MUSIi007-A) exhibited a normal karyotype, expressed pluripotent markers and displayed in vitro and in vivo differentiation potential into cells of three embryonic germ layers.Resource tableUnlabelled TableUnique stem cell line identifierMUSIi007-AAlternative name(s) of stem cell lineSeV-iPSCInstitutionSiriraj Center of Excellence for Stem Cell Research,Faculty of Medicine Siriraj Hospital, Mahidol UniversityContact information of distributorPonthip Potirat, ponthip.pot@gmail.comSurapol Issaragrisil, surapolsi@gmail.comType of cell lineiPSCsOriginHumanAdditional origin infoAge: 26-year-oldSex: MaleEthnicity: ThaiCell sourcePeripheral blood mononuclear cellsClonalityClonalMethod of reprogrammingIntegration-free Sendai viral vectorsGenetic modificationN/AType of modificationN/AAssociated diseaseN/AGene/locusN/AMethod of modificationN/AName of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock dateDecember 2014Cell line repository/bankN/AEthical approvalSiriraj Institutional Review Board (SiRB), no. Si248/2011, Faculty of Medicine Siriraj Hospital, Mahidol University, Thailand.
Author Kheolamai, Pakpoom
Wattanapanitch, Methichit
Potirat, Ponthip
Issaragrisil, Surapol
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References Wattanapanitch, Klincumhom, Potirat, Amornpisutt, Lorthongpanich, U-pratya, Laowtammathron, Kheolamai, Poungvarin, Issaragrisil (bb0005) 2014; 9
Wattanapanitch (10.1016/j.scr.2018.08.014_bb0005) 2014; 9
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  article-title: Dual small-molecule targeting of SMAD signaling stimulates human induced pluripotent stem cells toward neural lineages
  publication-title: PLoS One
– volume: 9
  issue: 9
  year: 2014
  ident: 10.1016/j.scr.2018.08.014_bb0005
  article-title: Dual small-molecule targeting of SMAD signaling stimulates human induced pluripotent stem cells toward neural lineages
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0106952
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SubjectTerms Cell Differentiation - genetics
Cell Differentiation - physiology
Cell Line
Cells, Cultured
Cellular Reprogramming - genetics
Cellular Reprogramming - physiology
Genetic Vectors - genetics
Humans
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Karyotype
Leukocytes, Mononuclear - cytology
Leukocytes, Mononuclear - metabolism
Sendai virus - genetics
Title Establishment of a human iPSC line (MUSIi007-A) from peripheral blood of normal individual using Sendai viral vectors
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