A Single ssRNA Segment Encoding RdRp Is Sufficient for Replication, Infection, and Transmission of Ourmia-Like Virus in Fungi

Recently, an increasing number of ourmia-like viruses have been found in fungi; however, the features of these viruses remain unknown. Here, we report a novel ourmia-like virus isolated from . This virus, named ourmia-like virus 4 (SsOLV4), has a genome 2,982 nt in length with a G-pentamer (GGGGG) a...

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Published in:Frontiers in microbiology Vol. 11; p. 379
Main Authors: Wang, Qihua, Mu, Fan, Xie, Jiatao, Cheng, Jiasen, Fu, Yanping, Jiang, Daohong
Format: Journal Article
Language:English
Published: Switzerland Frontiers Media S.A 18.03.2020
Subjects:
Botourmiaviridae
Microbiology
mycovirus
ourmia-like virus
Sclerotinia sclerotiorum
transfection
Sclerotinia sclerotiorum
mycovirus
ourmia-like virus
Botourmiaviridae
transfection
ISSN:1664-302X, 1664-302X
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Summary:Recently, an increasing number of ourmia-like viruses have been found in fungi; however, the features of these viruses remain unknown. Here, we report a novel ourmia-like virus isolated from . This virus, named ourmia-like virus 4 (SsOLV4), has a genome 2,982 nt in length with a G-pentamer (GGGGG) at the 5'-terminus and a C-pentamer (CCCCC) at the 3'-terminus. The SsOLV4 genome has only one large putative open reading frame (ORF) predicted with both standard codes and mitochondrial codes and encodes an RNA-dependent RNA polymerase (RdRp). SsOLV4 is closely phylogenetically related to ourmia-like virus 1, with 42% identity between the RdRp amino acid sequences. We constructed full-length cDNA of SsOLV4 and synthesized RNA using the T7 RNA polymerase. The synthesized RNA could transfect protoplasts efficiently. We further found that viral RNA could infect mycelia when mixed with PEG buffer. Our study suggests that a novel genus in family should be established for SsOLV4 and other related viruses and demonstrates that one single-stranded RNA segment encoding RdRp is sufficient for ourmia-like viruses in fungi.
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Reviewed by: Maria A. Ayllón, Polytechnic University of Madrid, Spain; Hiromitsu Moriyama, Tokyo University of Agriculture and Technology, Japan; Mahmoud E. Khalifa, Damietta University, Egypt
This article was submitted to Virology, a section of the journal Frontiers in Microbiology
Edited by: Nobuhiro Suzuki, Okayama University, Japan
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2020.00379