Molecular detection of nine rice viruses by a reverse-transcription loop-mediated isothermal amplification assay

A reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay was established for the detection of nine viruses from infected rice plants, including rice black-streaked dwarf virus (RBSDV), rice dwarf virus (RDV), rice gall dwarf virus (RGDV), rice ragged stunt virus (RRSV), rice tr...

Full description

Saved in:
Bibliographic Details
Published in:Journal of virological methods Vol. 170; no. 1-2; pp. 90 - 93
Main Authors: Le, Dung Tien, Netsu, Osamu, Uehara-Ichiki, Tamaki, Shimizu, Takumi, Choi, Il-Ryong, Omura, Toshihiro, Sasaya, Takahide
Format: Journal Article
Language:English
Published: Kidlington Elsevier B.V 01.12.2010
Elsevier
Subjects:
Agriculture
Animals
Base Sequence
Biological and medical sciences
Conserved Sequence
Detection
DNA Primers
DNA, Viral - genetics
Fundamental and applied biological sciences. Psychology
Insect Vectors - virology
Microbiology
Nucleic Acid Amplification Techniques - methods
Oryza - virology
Oryza sativa
Plant Leaves - virology
Plant Viruses - genetics
Plant Viruses - isolation & purification
Reverse Transcription
Rice dwarf virus
Rice gall dwarf virus
Rice grassy stunt virus
Rice ragged stunt virus
Rice stripe virus
Rice tungro bacilliform virus
Rice tungro spherical virus
Rice virus
RT-LAMP
Sensitivity and Specificity
Techniques used in virology
Viral Structural Proteins - genetics
Virology
RT-LAMP
RTBV
RGDV
RTSV
RSV
RDV
RRSV
RBSDV
RTYV
Rice virus
Detection
RGSV
Oryza sativa
Monocotyledones
Transcription
Loop mediated isothermal amplification reaction
Gramineae
Angiospermae
Spermatophyta
Method
Cereal crop
ISSN:0166-0934, 1879-0984, 1879-0984
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay was established for the detection of nine viruses from infected rice plants, including rice black-streaked dwarf virus (RBSDV), rice dwarf virus (RDV), rice gall dwarf virus (RGDV), rice ragged stunt virus (RRSV), rice transitory yellowing virus (RTYV), rice stripe virus (RSV), rice grassy stunt virus (RGSV), rice tungro spherical virus (RTSV), and rice tungro bacilliform virus (RTBV). Virus-specific primer sets were designed from the genome sequences of these viruses. By the combination of RNA rapid extraction and RT-LAMP, these nine viruses could be detected within 2h from infected rice plants. The sensitivities of the assays were either higher than (for RSV, RTBV, and RTYV) or similar (for RDV) to those of one-step RT-PCR. Furthermore, RTBV and RTSV were detected not only in infected rice plants but also in viruliferous insect vectors. The RT-LAMP assays may facilitate studies on rice disease epidemiology, outbreak surveillance, and molecular pathology.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ObjectType-Article-2
ObjectType-Feature-1
ISSN:0166-0934
1879-0984
1879-0984
DOI:10.1016/j.jviromet.2010.09.004