Molecular detection of potential sexually transmitted pathogens in semen and urine specimens of infertile and fertile males

A total of 93 infertile and 70 fertile men attending various urology and gynecology clinics in Jordan were investigated in this prospective study. First void urine and the corresponding semen specimens were collected from 96% of the patients. Presence of Neisseria gonorrhoeae (NG), Chlamydia trachom...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:Diagnostic microbiology and infectious disease Ročník 77; číslo 4; s. 283 - 286
Hlavní autoři: Abusarah, Eman A., Awwad, Ziad M., Charvalos, Ekatherina, Shehabi, Asem A.
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States Elsevier Inc 01.12.2013
Témata:
Adult
Bacteriuria - diagnosis
Bacteriuria - microbiology
Chlamydia trachomatis
Chlamydia trachomatis - genetics
DNA
DNA, Bacterial
Humans
Infectious Disease
Infertility, Male - epidemiology
Infertility, Male - etiology
Internal Medicine
Jordan
Jordan - epidemiology
Male
Male infertility
males
men
Mycoplasma genitalium
Mycoplasma genitalium - genetics
Neisseria gonorrhoeae
Neisseria gonorrhoeae - genetics
pathogens
patients
PCR
Polymerase Chain Reaction
Prevalence
prospective studies
semen
Semen - microbiology
Sexually Transmitted Diseases, Bacterial - complications
Sexually Transmitted Diseases, Bacterial - diagnosis
Sexually Transmitted Diseases, Bacterial - epidemiology
Ureaplasma parvum
Ureaplasma urealyticum
Ureaplasma urealyticum - genetics
urine
Jordan
Ureaplasma urealyticum
Chlamydia trachomatis
Mycoplasma genitalium
Neisseria gonorrhoeae
Male infertility
PCR
ISSN:0732-8893, 1879-0070, 1879-0070
On-line přístup:Získat plný text
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
Popis
Shrnutí:A total of 93 infertile and 70 fertile men attending various urology and gynecology clinics in Jordan were investigated in this prospective study. First void urine and the corresponding semen specimens were collected from 96% of the patients. Presence of Neisseria gonorrhoeae (NG), Chlamydia trachomatis (CT), Ureaplasma urealyticum (UU), and Mycoplasma genitalium (MG) DNA in specimen was detected using polymerase chain reaction. The distribution of NG, CT, UU, and MG in semen and FVU specimens among infertile versus fertile men was 6.5% versus 0%, 4.3% versus 1.4%, 10.8% versus 5.7%, and 3.2% versus 1.4%, respectively. Two of infertile and 1 of fertile men harbored mixed pathogens. The highest number of positive potential pathogens was found among young men aged 20–29 years old. The present study found a very high concordance between the detection of CT, UU, and MG DNA in semen and the corresponding FVU specimens, while NG DNA found only in semen and not in the corresponding FVU specimens. This study also revealed that Ureaplasma parvum species is more prevalent than Ureaplasma urealyticum in specimens of infertile men (90%). The study demonstrates that infertile men have higher prevalence of NG, CT, UU, and MG compared with fertile men and NG as significantly associated with infertile men.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0732-8893
1879-0070
1879-0070
DOI:10.1016/j.diagmicrobio.2013.05.018