Characterization of Newly Isolated Lytic Bacteriophages Active against Acinetobacter baumannii

Based on genotyping and host range, two newly isolated lytic bacteriophages, myovirus vB_AbaM_Acibel004 and podovirus vB_AbaP_Acibel007, active against Acinetobacter baumannii clinical strains, were selected from a new phage library for further characterization. The complete genomes of the two phage...

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Veröffentlicht in:PloS one Jg. 9; H. 8; S. e104853
Hauptverfasser: Merabishvili, Maia, Vandenheuvel, Dieter, Kropinski, Andrew M., Mast, Jan, De Vos, Daniel, Verbeken, Gilbert, Noben, Jean-Paul, Lavigne, Rob, Vaneechoutte, Mario, Pirnay, Jean-Paul
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States Public Library of Science 11.08.2014
Public Library of Science (PLoS)
Schlagworte:
Acinetobacter
Acinetobacter baumannii
Acinetobacter baumannii - virology
Antibiotics
Bacteriology
Bacteriophages - genetics
Bacteriophages - growth & development
Bacteriophages - isolation & purification
Bacteriophages - physiology
Biology and Life Sciences
Burst size
Cell culture
Culture Techniques
Drug resistance
Epidemics
Genes
Genome, Viral - genetics
Genomes
Genomic analysis
Genotyping
Health sciences
Hospitals
Host range
Host Specificity
Infections
Laboratories
Latent period
Liquid culture
Molecular Sequence Annotation
Phages
Phenotype
Proteomics
Pseudomonas aeruginosa
Public health
Resistant mutant
Transfer RNA
Canada
Belgium
France
Union of Soviet Socialist Republics > USSR
Guelph Ontario Canada
ISSN:1932-6203, 1932-6203
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Zusammenfassung:Based on genotyping and host range, two newly isolated lytic bacteriophages, myovirus vB_AbaM_Acibel004 and podovirus vB_AbaP_Acibel007, active against Acinetobacter baumannii clinical strains, were selected from a new phage library for further characterization. The complete genomes of the two phages were analyzed. Both phages are characterized by broad host range and essential features of potential therapeutic phages, such as short latent period (27 and 21 min, respectively), high burst size (125 and 145, respectively), stability of activity in liquid culture and low frequency of occurrence of phage-resistant mutant bacterial cells. Genomic analysis showed that while Acibel004 represents a novel bacteriophage with resemblance to some unclassified Pseudomonas aeruginosa phages, Acibel007 belongs to the well-characterized genus of the Phikmvlikevirus. The newly isolated phages can serve as potential candidates for phage cocktails to control A. baumannii infections.
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Conceived and designed the experiments: MM DV AK RL JPP. Performed the experiments: MM DV JM. Analyzed the data: MM DV AK DDV JPN MV JPP. Contributed reagents/materials/analysis tools: MM DV AK JM GV JPN RL MV JPP. Wrote the paper: MM DV RL MV JPP.
Competing Interests: The authors have declared that no competing interests exist.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0104853