Amplified Fragment Length Polymorphism Mapping of Quantitative Trait Loci for Malaria Parasite Susceptibility in the Yellow Fever Mosquito Aedes aegypti

The yellow fever mosquito Aedes aegypti has been the subject of extensive genetic research due to its medical importance and the ease with which it can be manipulated in the laboratory. A molecular genetic linkage map was constructed using 148 amplified fragment length polymorphism (AFLP) and six si...

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Veröffentlicht in:	Genetics (Austin) Jg. 173; H. 3; S. 1337 - 1345
Hauptverfasser:	Zhong, Daibin, Menge, David M, Temu, Emmanuel A, Chen, Hong, Yan, Guiyun
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States Genetics Soc America 01.07.2006 Genetics Society of America Copyright © 2006 by the Genetics Society of America
Schlagworte:	Aedes - genetics Aedes - parasitology Aedes aegypti Animals Aquatic insects Chromosome Mapping Chromosomes Cloning Deoxyribonucleic acid DNA DNA Primers Epistasis, Genetic Gene mapping Genes Genetic engineering Genetic linkage Genetic research Genomics Infectious diseases Insect Vectors - genetics Insect Vectors - parasitology Investigations Malaria Mosquitoes Parasites Phenotype Plasmodium Plasmodium gallinaceum Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Quantitative Trait Loci Sample size Tropical diseases Vector-borne diseases Yellow Fever - transmission
ISSN:	0016-6731, 1943-2631, 1943-2631
Online-Zugang:	Volltext
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Zusammenfassung:	The yellow fever mosquito Aedes aegypti has been the subject of extensive genetic research due to its medical importance and the ease with which it can be manipulated in the laboratory. A molecular genetic linkage map was constructed using 148 amplified fragment length polymorphism (AFLP) and six single-strand conformation polymorphism (SSCP) markers. Eighteen AFLP primer combinations were used to genotype two reciprocal F2 segregating populations. Each primer combination generated an average of 8.2 AFLP markers eligible for linkage mapping. The length of the integrated map was 180.9 cM, giving an average marker resolution of 1.2 cM. Composite interval mapping revealed a total of six QTL significantly affecting Plasmodium susceptibility in the two reciprocal crosses of Ae. aegypti. Two common QTL on linkage group 2 were identified in both crosses that had similar effects on the phenotype, and four QTL were unique to each cross. In one cross, the four main QTL accounted for 64% of the total phenotypic variance, and digenic epistasis explained 11.8% of the variance. In the second cross, the four main QTL explained 66% of the variance, and digenic epistasis accounted for 16% of the variance. The actions of these QTL were either dominance or underdominance. Our results indicated that at least three new QTL were mapped on chromosomes 1 and 3. The polygenic nature of susceptibility to P. gallinaceum and epistasis are important factors for significant variation within or among mosquito strains. The new map provides additional information useful for further genetic investigation, such as identification of new genes and positional cloning.
Bibliographie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 ObjectType-Article-2 ObjectType-Feature-1 content type line 23 Corresponding author: Program in Public Health, College of Health Sciences, University of California, 3501 Hewitt Hall, Irvine, CA 92697-4050. E-mail: dzhong@uci.edu Communicating editor: J. A. Birchler Present address: The Institute of Tropical Medicine, Nagasaki University, Nagasaki-shi 852-8523, Japan.
ISSN:	0016-6731 1943-2631 1943-2631
DOI:	10.1534/genetics.105.055178