Optimizing PCR positivity criterion for detection of herpes simplex virus DNA on skin and mucosa

In 1997, we developed a PCR assay for the detection of herpes simplex virus (HSV) DNA. Recently, we determined an optimal positivity criterion based on research specimens and a dilution study. We found that a cutoff of 50 HSV DNA copies/ml of swab specimen, a level 10-fold lower than our previous cu...

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Vydané v:Journal of clinical microbiology Ročník 45; číslo 5; s. 1618
Hlavní autori: Magaret, Amalia S, Wald, Anna, Huang, Meei-Li, Selke, Stacy, Corey, Larry
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: United States 01.05.2007
Predmet:
DNA, Viral - genetics
DNA, Viral - isolation & purification
False Positive Reactions
Humans
Mucous Membrane - virology
Polymerase Chain Reaction - methods
Polymerase Chain Reaction - standards
Reproducibility of Results
Sensitivity and Specificity
Simplexvirus - genetics
Simplexvirus - isolation & purification
Skin - virology
Virus Shedding
ISSN:0095-1137
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Popis
Shrnutí:In 1997, we developed a PCR assay for the detection of herpes simplex virus (HSV) DNA. Recently, we determined an optimal positivity criterion based on research specimens and a dilution study. We found that a cutoff of 50 HSV DNA copies/ml of swab specimen, a level 10-fold lower than our previous cutoff, minimizes misclassification.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0095-1137
DOI:10.1128/JCM.01405-06