Optimizing PCR positivity criterion for detection of herpes simplex virus DNA on skin and mucosa

In 1997, we developed a PCR assay for the detection of herpes simplex virus (HSV) DNA. Recently, we determined an optimal positivity criterion based on research specimens and a dilution study. We found that a cutoff of 50 HSV DNA copies/ml of swab specimen, a level 10-fold lower than our previous cu...

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Veröffentlicht in:	Journal of clinical microbiology Jg. 45; H. 5; S. 1618
Hauptverfasser:	Magaret, Amalia S, Wald, Anna, Huang, Meei-Li, Selke, Stacy, Corey, Larry
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States 01.05.2007
Schlagworte:	DNA, Viral - genetics DNA, Viral - isolation & purification False Positive Reactions Humans Mucous Membrane - virology Polymerase Chain Reaction - methods Polymerase Chain Reaction - standards Reproducibility of Results Sensitivity and Specificity Simplexvirus - genetics Simplexvirus - isolation & purification Skin - virology Virus Shedding
ISSN:	0095-1137
Online-Zugang:	Weitere Angaben
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