Optimizing PCR positivity criterion for detection of herpes simplex virus DNA on skin and mucosa

In 1997, we developed a PCR assay for the detection of herpes simplex virus (HSV) DNA. Recently, we determined an optimal positivity criterion based on research specimens and a dilution study. We found that a cutoff of 50 HSV DNA copies/ml of swab specimen, a level 10-fold lower than our previous cu...

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Published in:Journal of clinical microbiology Vol. 45; no. 5; p. 1618
Main Authors: Magaret, Amalia S, Wald, Anna, Huang, Meei-Li, Selke, Stacy, Corey, Larry
Format: Journal Article
Language:English
Published: United States 01.05.2007
Subjects:
DNA, Viral - genetics
DNA, Viral - isolation & purification
False Positive Reactions
Humans
Mucous Membrane - virology
Polymerase Chain Reaction - methods
Polymerase Chain Reaction - standards
Reproducibility of Results
Sensitivity and Specificity
Simplexvirus - genetics
Simplexvirus - isolation & purification
Skin - virology
Virus Shedding
ISSN:0095-1137
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Abstract In 1997, we developed a PCR assay for the detection of herpes simplex virus (HSV) DNA. Recently, we determined an optimal positivity criterion based on research specimens and a dilution study. We found that a cutoff of 50 HSV DNA copies/ml of swab specimen, a level 10-fold lower than our previous cutoff, minimizes misclassification.
AbstractList In 1997, we developed a PCR assay for the detection of herpes simplex virus (HSV) DNA. Recently, we determined an optimal positivity criterion based on research specimens and a dilution study. We found that a cutoff of 50 HSV DNA copies/ml of swab specimen, a level 10-fold lower than our previous cutoff, minimizes misclassification.In 1997, we developed a PCR assay for the detection of herpes simplex virus (HSV) DNA. Recently, we determined an optimal positivity criterion based on research specimens and a dilution study. We found that a cutoff of 50 HSV DNA copies/ml of swab specimen, a level 10-fold lower than our previous cutoff, minimizes misclassification.
In 1997, we developed a PCR assay for the detection of herpes simplex virus (HSV) DNA. Recently, we determined an optimal positivity criterion based on research specimens and a dilution study. We found that a cutoff of 50 HSV DNA copies/ml of swab specimen, a level 10-fold lower than our previous cutoff, minimizes misclassification.
Author Huang, Meei-Li
Magaret, Amalia S
Corey, Larry
Selke, Stacy
Wald, Anna
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SubjectTerms DNA, Viral - genetics
DNA, Viral - isolation & purification
False Positive Reactions
Humans
Mucous Membrane - virology
Polymerase Chain Reaction - methods
Polymerase Chain Reaction - standards
Reproducibility of Results
Sensitivity and Specificity
Simplexvirus - genetics
Simplexvirus - isolation & purification
Skin - virology
Virus Shedding
Title Optimizing PCR positivity criterion for detection of herpes simplex virus DNA on skin and mucosa
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