Characterisation of molecular motions in cryo-EM single-particle data by multi-body refinement in RELION

Macromolecular complexes that exhibit continuous forms of structural flexibility pose a challenge for many existing tools in cryo-EM single-particle analysis. We describe a new tool, called multi-body refinement, which models flexible complexes as a user-defined number of rigid bodies that move inde...

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Vydáno v:	eLife Ročník 7
Hlavní autoři:	Nakane, Takanori, Kimanius, Dari, Lindahl, Erik, Scheres, Sjors HW
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	England eLife Sciences Publications, Ltd 01.06.2018 eLife Sciences Publications Ltd
Témata:	Biochemistry towards Bioinformatics biokemi med inriktning mot bioinformatik cryo-electron microscopy Cryoelectron Microscopy focused refinement image processing Image Processing, Computer-Assisted maximum likelihood Models, Molecular Motion Plasmodium falciparum - metabolism Ribosomes - metabolism Saccharomyces cerevisiae single-particle analysis Software Spliceosomes - metabolism Structural Biology and Molecular Biophysics Tools and Resources image processing focused refinement maximum likelihood structural biology molecular biophysics S. cerevisiae cryo-electron microscopy single-particle analysis
ISSN:	2050-084X, 2050-084X
On-line přístup:	Získat plný text
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