3D Correlative Cryo-Structured Illumination Fluorescence and Soft X-ray Microscopy Elucidates Reovirus Intracellular Release Pathway

Imaging of biological matter across resolution scales entails the challenge of preserving the direct and unambiguous correlation of subject features from the macroscopic to the microscopic level. Here, we present a correlative imaging platform developed specifically for imaging cells in 3D under cry...

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Vydané v:	Cell Ročník 182; číslo 2; s. 515
Hlavní autori:	Kounatidis, Ilias, Stanifer, Megan L, Phillips, Michael A, Paul-Gilloteaux, Perrine, Heiligenstein, Xavier, Wang, Hongchang, Okolo, Chidinma A, Fish, Thomas M, Spink, Matthew C, Stuart, David I, Davis, Ilan, Boulant, Steeve, Grimes, Jonathan M, Dobbie, Ian M, Harkiolaki, Maria
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	United States 23.07.2020
Predmet:	Cell Line, Tumor Cryoelectron Microscopy - instrumentation Cryoelectron Microscopy - methods Endosomes - metabolism Endosomes - virology Fluorescent Dyes - chemistry Humans Imaging, Three-Dimensional Microscopy, Fluorescence Reoviridae - chemistry Reoviridae - physiology Virus Release - physiology X-ray tomography reovirus biology CLXT structured illumination microscopy correlative imaging
ISSN:	1097-4172, 1097-4172
On-line prístup:	Zistit podrobnosti o prístupe
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