Role of host cell traversal by the malaria sporozoite during liver infection

Malaria infection starts when the sporozoite stage of the Plasmodium parasite is injected into the skin by a mosquito. Sporozoites are known to traverse host cells before finally invading a hepatocyte and multiplying into erythrocyte-infecting forms, but how sporozoites reach hepatocytes in the live...

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Veröffentlicht in:The Journal of experimental medicine Jg. 210; H. 5; S. 905
Hauptverfasser: Tavares, Joana, Formaglio, Pauline, Thiberge, Sabine, Mordelet, Elodie, Van Rooijen, Nico, Medvinsky, Alexander, Ménard, Robert, Amino, Rogerio
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States 06.05.2013
Schlagworte:
Animals
Anopheles - parasitology
Cell Death
Cell Movement
Endothelial Cells - parasitology
Endothelial Cells - pathology
Female
Green Fluorescent Proteins - metabolism
Host-Parasite Interactions - immunology
Kupffer Cells - parasitology
Kupffer Cells - pathology
Liver - parasitology
Liver - pathology
Malaria - parasitology
Malaria - pathology
Male
Mice
Mice, Inbred C57BL
Plasmodium berghei - cytology
Plasmodium berghei - physiology
Sporozoites - cytology
Sporozoites - physiology
ISSN:1540-9538, 1540-9538
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Zusammenfassung:Malaria infection starts when the sporozoite stage of the Plasmodium parasite is injected into the skin by a mosquito. Sporozoites are known to traverse host cells before finally invading a hepatocyte and multiplying into erythrocyte-infecting forms, but how sporozoites reach hepatocytes in the liver and the role of host cell traversal (CT) remain unclear. We report the first quantitative imaging study of sporozoite liver infection in rodents. We show that sporozoites can cross the liver sinusoidal barrier by multiple mechanisms, targeting Kupffer cells (KC) or endothelial cells and associated or not with the parasite CT activity. We also show that the primary role of CT is to inhibit sporozoite clearance by KC during locomotion inside the sinusoid lumen, before crossing the barrier. By being involved in multiple steps of the sporozoite journey from the skin to the final hepatocyte, the parasite proteins mediating host CT emerge as ideal antibody targets for vaccination against the parasite.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1540-9538
1540-9538
DOI:10.1084/jem.20121130