Protein identification strategies in MALDI imaging mass spectrometry: a brief review

[Display omitted] Matrix assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) is a powerful technology used to investigate the spatial distributions of thousands of molecules throughout a tissue section from a single experiment. As proteins represent an important group of fun...

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Bibliographic Details
Published in:	Current opinion in chemical biology Vol. 48; pp. 64 - 72
Main Authors:	Ryan, Daniel J, Spraggins, Jeffrey M, Caprioli, Richard M
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 01.02.2019
Subjects:	Animals Equipment Design Humans Molecular Imaging - instrumentation Molecular Imaging - methods Proteins - analysis Proteins - isolation & purification Specimen Handling - instrumentation Specimen Handling - methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - instrumentation Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Workflow
ISSN:	1367-5931, 1879-0402, 1879-0402
Online Access:	Get full text
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Summary:	[Display omitted] Matrix assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) is a powerful technology used to investigate the spatial distributions of thousands of molecules throughout a tissue section from a single experiment. As proteins represent an important group of functional molecules in tissue and cells, the imaging of proteins has been an important point of focus in the development of IMS technologies and methods. Protein identification is crucial for the biological contextualization of molecular imaging data. However, gas-phase fragmentation efficiency of MALDI generated proteins presents significant challenges, making protein identification directly from tissue difficult. This review highlights methods and technologies specifically related to protein identification that have been developed to overcome these challenges in MALDI IMS experiments.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 ObjectType-Review-3 content type line 23
ISSN:	1367-5931 1879-0402 1879-0402
DOI:	10.1016/j.cbpa.2018.10.023