Multiplexed In Situ Imaging Mass Cytometry Analysis of the Human Endocrine Pancreas and Immune System in Type 1 Diabetes

The interaction between the immune system and endocrine cells in the pancreas is crucial for the initiation and progression of type 1 diabetes (T1D). Imaging mass cytometry (IMC) enables multiplexed assessment of the abundance and localization of more than 30 proteins on the same tissue section at 1...

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Bibliographic Details
Published in:Cell metabolism Vol. 29; no. 3; p. 769
Main Authors: Wang, Yue J, Traum, Daniel, Schug, Jonathan, Gao, Long, Liu, Chengyang, Atkinson, Mark A, Powers, Alvin C, Feldman, Michael D, Naji, Ali, Chang, Kyong-Mi, Kaestner, Klaus H
Format: Journal Article
Language:English
Published: United States 05.03.2019
Subjects:
Diabetes Mellitus, Type 1 - immunology
Diabetes Mellitus, Type 1 - metabolism
Endocrine Cells - immunology
Endocrine Cells - metabolism
Humans
Image Cytometry - methods
Immune System - immunology
Immune System - metabolism
Islets of Langerhans - immunology
Islets of Langerhans - metabolism
Islets of Langerhans - ultrastructure
type 1 diabetes
T1D
immunolabeling
histopathology
imaging mass cytometry
immune cell composition
spatial information
imaging
islet structure
multiplexed imaging
human pancreas
ISSN:1932-7420, 1932-7420
Online Access:Get more information
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Summary:The interaction between the immune system and endocrine cells in the pancreas is crucial for the initiation and progression of type 1 diabetes (T1D). Imaging mass cytometry (IMC) enables multiplexed assessment of the abundance and localization of more than 30 proteins on the same tissue section at 1-μm resolution. Herein, we have developed a panel of 33 antibodies that allows for the quantification of key cell types including pancreatic exocrine cells, islet cells, immune cells, and stromal components. We employed this panel to analyze 12 pancreata obtained from donors with clinically diagnosed T1D and 6 pancreata from non-diabetic controls. In the pancreata from donors with T1D, we simultaneously visualized significant alterations in islet architecture, endocrine cell composition, and immune cell presentation. Indeed, we demonstrate the utility of IMC to investigate complex events on the cellular level that will provide new insights on the pathophysiology of T1D.
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ISSN:1932-7420
1932-7420
DOI:10.1016/j.cmet.2019.01.003