G-quadruplex Structures Contribute to Differential Radiosensitivity of the Human Genome

DNA, the fundamental unit of human cell, generally exists in Watson-Crick base-paired B-DNA form. Often, DNA folds into non-B forms, such as four-stranded G-quadruplexes. It is generally believed that ionizing radiation (IR) induces DNA strand-breaks in a random manner. Here, we show that regions of...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:iScience Ročník 21; s. 288 - 307
Hlavní autoři: Kumari, Nitu, Vartak, Supriya V., Dahal, Sumedha, Kumari, Susmita, Desai, Sagar S., Gopalakrishnan, Vidya, Choudhary, Bibha, Raghavan, Sathees C.
Médium: Journal Article
Jazyk:angličtina
Vydáno: United States Elsevier Inc 22.11.2019
Elsevier
Témata:
Biochemistry
Biological Sciences
Cell Biology
Molecular Biology
Biological Sciences
Biochemistry
Molecular Biology
Cell Biology
ISSN:2589-0042, 2589-0042
On-line přístup:Získat plný text
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
Popis
Shrnutí:DNA, the fundamental unit of human cell, generally exists in Watson-Crick base-paired B-DNA form. Often, DNA folds into non-B forms, such as four-stranded G-quadruplexes. It is generally believed that ionizing radiation (IR) induces DNA strand-breaks in a random manner. Here, we show that regions of DNA enriched in G-quadruplex structures are less sensitive to IR compared with B-DNA in vitro and inside cells. Planar G-quartet of G4-DNA is shielded from IR-induced free radicals, unlike single- and double-stranded DNA. Whole-genome sequence analysis and real-time PCR reveal that genomic regions abundant in G4-DNA are protected from radiation-induced breaks and can be modulated by G4 stabilizers. Thus, our results reveal that formation of G4 structures contribute toward differential radiosensitivity of the human genome. [Display omitted] •G4 DNA contributes to genome-wide radioprotection and is modulated by G4 resolvases•Radiation causes minimal damage at the G4 structures at telomeres•Formation of G4 DNA contributes toward differential radiosensitivity of human genome•Planar quartet of G4 DNA is shielded from IR-induced free radicals and thus DNA breaks Biological Sciences; Biochemistry; Molecular Biology; Cell Biology
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Lead Contact
These authors contributed equally
ISSN:2589-0042
2589-0042
DOI:10.1016/j.isci.2019.10.033