Immunofluorescence microscopy-based detection of ssDNA foci by BrdU in mammalian cells

DNA end resection converts broken ends of double-stranded DNA (dsDNA) to 3′-single-stranded DNA (3′-ssDNA). The extent of resection regulates DNA double-strand break (DSB) repair pathway choice and thereby genomic stability. Here, we characterize an optimized immunofluorescence (IF) microscopy-based...

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Bibliographic Details
Published in:	STAR protocols Vol. 2; no. 4; p. 100978
Main Authors:	Kilgas, Susan, Kiltie, Anne E., Ramadan, Kristijan
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 17.12.2021 Elsevier
Subjects:	Antibody Bromodeoxyuridine - chemistry Bromodeoxyuridine - metabolism Cell Biology Cell Line, Tumor Cell-based Assays DNA, Single-Stranded - analysis DNA, Single-Stranded - chemistry DNA, Single-Stranded - genetics DNA, Single-Stranded - metabolism Genomic Instability - genetics Humans Microscopy Microscopy, Fluorescence - methods Molecular Biology Protocol Microscopy Molecular Biology Antibody Cell-based Assays Cell Biology
ISSN:	2666-1667, 2666-1667
Online Access:	Get full text
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