Consensus recommendations for trabecular meshwork cell isolation, characterization and culture

Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedure...

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Published in:	Experimental eye research Vol. 171; pp. 164 - 173
Main Authors:	Keller, Kate E., Bhattacharya, Sanjoy K., Borrás, Theresa, Brunner, Thomas M., Chansangpetch, Sunee, Clark, Abbott F., Dismuke, W. Michael, Du, Yiqin, Elliott, Michael H., Ethier, C. Ross, Faralli, Jennifer A., Freddo, Thomas F., Fuchshofer, Rudolf, Giovingo, Michael, Gong, Haiyan, Gonzalez, Pedro, Huang, Alex, Johnstone, Murray A., Kaufman, Paul L., Kelley, Mary J., Knepper, Paul A., Kopczynski, Casey C., Kuchtey, John G., Kuchtey, Rachel W., Kuehn, Markus H., Lieberman, Raquel L., Lin, Shan C., Liton, Paloma, Liu, Yutao, Lütjen-Drecoll, Elke, Mao, Weiming, Masis-Solano, Marisse, McDonnell, Fiona, McDowell, Colleen M., Overby, Darryl R., Pattabiraman, Padmanabhan P., Raghunathan, Vijay K., Rao, P. Vasanth, Rhee, Douglas J., Chowdhury, Uttio Roy, Russell, Paul, Samples, John R., Schwartz, Donald, Stubbs, Evan B., Tamm, Ernst R., Tan, James C., Toris, Carol B., Torrejon, Karen Y., Vranka, Janice A., Wirtz, Mary K., Yorio, Thomas, Zhang, Jie, Zode, Gulab S., Fautsch, Michael P., Peters, Donna M., Acott, Ted S., Stamer, W. Daniel
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 01.06.2018
Subjects:	Age Factors Animals Aqueous humor dynamics Biomarkers - metabolism Cell Culture Techniques Cell Separation - methods Consensus Conventional outflow Fetus Glaucoma Guidelines as Topic Humans Intraocular pressure Ocular hypertension Tissue and Organ Harvesting Tissue Donors Tissue Preservation Trabecular Meshwork - cytology Trabecular Meshwork - metabolism Glaucoma Conventional outflow Fibroblast growth factor Phosphate-buffered saline Interleukin Ocular hypertension Matrix gla protein Fetal bovine serum Dulbecco's modified Eagle's medium Ethylenediaminetetraacetic acid Juxtacanalicular tissue Trabecular meshwork Aqueous humor dynamics Tumor necrosis factor Schlemm's canal Dimethyl sulfoxide Institutional review board Human immunodeficiency virus Intraocular pressure
ISSN:	0014-4835, 1096-0007, 1096-0007
Online Access:	Get full text
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Summary:	Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods used to validate their identity. Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings. •Comprehensive review of techniques to culture TM cells are presented.•Helpful tips for culturing TM cells are described.•First set of consensus standards for TM cell research is defined.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 ObjectType-Review-3 content type line 23
ISSN:	0014-4835 1096-0007 1096-0007
DOI:	10.1016/j.exer.2018.03.001