[18F]FDG and [18F]FLT uptake in human breast cancer cells in relation to the effects of chemotherapy: an in vitro study

Increased 2′-deoxy-2′-[ 18 F]fluoro- D -glucose (FDG) uptake is the most commonly used marker for positron emission tomography in oncology. However, a proliferation tracer such as 3′-deoxy-3′-[ 18 F]fluorothymidine (FLT) might be more specific for cancer. 3′-deoxy-3′-[ 18 F]fluorothymidine uptake is...

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Vydané v:British journal of cancer Ročník 99; číslo 3; s. 481 - 487
Hlavní autori: Direcks, W G E, Berndsen, S C, Proost, N, Peters, G J, Balzarini, J, Spreeuwenberg, M D, Lammertsma, A A, Molthoff, C F M
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: London Nature Publishing Group UK 05.08.2008
Nature Publishing Group
Predmet:
Antineoplastic Agents - pharmacology
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Breast cancer
Breast Neoplasms - enzymology
Breast Neoplasms - metabolism
Breast Neoplasms - pathology
Cancer Research
Cell cycle
Cell Cycle - drug effects
Cell division
Cell Line, Tumor
Chemotherapy
Cytotoxicity
Doxorubicin - pharmacology
Drug Resistance
Enzymes
Epidemiology
Fluorodeoxyglucose F18 - metabolism
Fluorouracil - pharmacology
Glucose
Gynecology. Andrology. Obstetrics
Humans
Kinases
Mammary gland diseases
Medical research
Medical sciences
Molecular Medicine
Oncology
Paclitaxel - pharmacology
Thymidine - metabolism
Thymidine Kinase - metabolism
Tomography
Translational Therapeutics
Tumors
United States > US
Netherlands
breast cancer cells
F]FLT
thymidine kinase
F]FDG
[
chemotherapy
PET
Radionuclide study
Human
Thymidine kinase
[18F]FDG
Breast disease
[18F]FLT
Enzyme
Transferases
Breast cancer
Malignant tumor
In vitro
Mammary gland diseases
Chemotherapy
Cancerology
Treatment
Positron emission tomography
Tumor cell
Cancer
ISSN:0007-0920, 1532-1827
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Shrnutí:Increased 2′-deoxy-2′-[ 18 F]fluoro- D -glucose (FDG) uptake is the most commonly used marker for positron emission tomography in oncology. However, a proliferation tracer such as 3′-deoxy-3′-[ 18 F]fluorothymidine (FLT) might be more specific for cancer. 3′-deoxy-3′-[ 18 F]fluorothymidine uptake is dependent on thymidine kinase 1 (TK) activity, but the effects of chemotherapeutic agents are unknown. The aim of this study was to characterise FDG and FLT uptake mechanisms in vitro before and after exposure to chemotherapeutic agents. The effects of 5-fluorouracil (5-FU), doxorubicin and paclitaxel on FDG and FLT uptake were measured in MDA MB231 human breast cancer cells in relation to cell cycle distribution, expression and enzyme activity of TK-1. At IC 50 concentrations, 5-FU resulted in accumulation in the G1 phase, but doxorubicin and paclitaxel induced a G2/M accumulation. Compared with untreated cells, 5-FU and doxorubicin increased TK-1 levels by >300. At 72 h, 5-FU decreased FDG uptake by 50% and FLT uptake by 54%, whereas doxorubicin increased FDG and FLT uptake by 71 and 173%, respectively. Paclitaxel increased FDG uptake with >100% after 48 h, whereas FLT uptake hardly changed. In conclusion, various chemotherapeutic agents, commonly used in the treatment of breast cancer, have different effects on the time course of uptake of both FDG and FLT in vitro . This might have implications for interpretation of clinical findings.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
ISSN:0007-0920
1532-1827
DOI:10.1038/sj.bjc.6604523