Complex expression changes of the placental endothelin system in early and late onset preeclampsia, fetal growth restriction and gestational diabetes

Preeclampsia (PE), fetal growth restriction (FGR) and gestational diabetes mellitus (GDM) are major pregnancy complications affecting maternal and fetal health. The placenta and the vasoconstrictor endothelin-1 (ET-1) have a controlling and mediating role in these conditions. This study tested the h...

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Bibliographic Details
Published in:Life sciences (1973) Vol. 91; no. 13-14; pp. 710 - 715
Main Authors: Dieber-Rotheneder, Martina, Beganovic, Sanja, Desoye, Gernot, Lang, Uwe, Cervar-Zivkovic, Mila
Format: Journal Article
Language:English
Published: Netherlands Elsevier Inc 15.10.2012
Subjects:
Adult
birth weight
blood flow
blood pressure
Case-Control Studies
Diabetes, Gestational - physiopathology
Down-Regulation
endothelin
Endothelin-1 - genetics
Female
fetal development
fetal growth restriction
Fetal Growth Retardation - physiopathology
gene expression
gene expression regulation
Gestational Age
gestational diabetes
Human placenta
Humans
immunoblotting
messenger RNA
placenta
Placenta - metabolism
Polymerase Chain Reaction
pre-eclampsia
Pre-Eclampsia - physiopathology
preeclampsia
Pregnancy
Pregnancy Trimester, Third
quantitative polymerase chain reaction
Receptor, Endothelin A - genetics
Receptor, Endothelin B - genetics
receptors
RNA, Messenger - metabolism
Up-Regulation
women
Young Adult
Human placenta
endothelin
preeclampsia
fetal growth restriction
gestational diabetes
ISSN:0024-3205, 1879-0631, 1879-0631
Online Access:Get full text
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Summary:Preeclampsia (PE), fetal growth restriction (FGR) and gestational diabetes mellitus (GDM) are major pregnancy complications affecting maternal and fetal health. The placenta and the vasoconstrictor endothelin-1 (ET-1) have a controlling and mediating role in these conditions. This study tested the hypothesis that the expression of ET-1 and its receptors (ETA and ETB) is altered in these pathologies and differs between early (gestational week [GW]≤34) and late (GW>34) third trimester pregnancies. The study included 88 women (GW 28-41) with PE (blood pressure >140/90mmHg, protein >300mg/24hrs; n=14), FGR (<10th birthweight centile and pathological umbilical blood flow; n=13), PE+FGR (n=5) and GDM (n=21), and gestational age-matched controls (n=35). ET-1, ETA and ETB mRNA and ETA and ETB protein were quantified in placental tissues by real-time PCR and immunoblotting. The ET/ETR mRNA system is altered in PE and PE+FGR and GDM. Expression of ET-1, ETA and ETB is upregulated in early onset PE and PE+FGR with stronger effect in PE+FGR. GDM down regulated ET/ETR mRNA in the placentas in late third trimester of pregnancy. ET/ETR protein is virtually unchanged. Early onset PE (≤GW34) with or without FGR is associated with increased mRNA expression of the ET/ETR system, while in late onset PE and GDM the opposite effect was observed. This study supports the emerging concept that early and late onset PE are different diseases.
Bibliography:http://dx.doi.org/10.1016/j.lfs.2012.04.040
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ISSN:0024-3205
1879-0631
1879-0631
DOI:10.1016/j.lfs.2012.04.040