Fixation methods for electron microscopy of human and other liver

For an electron microscopic study of the liver, expertise and complicated, time-consuming processing of hepatic tissues and cells is needed. The interpretation of electron microscopy (EM) images requires knowledge of the liver fine structure and experience with the numerous artifacts in fixation, em...

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Bibliographic Details
Published in:World journal of gastroenterology : WJG Vol. 16; no. 23; p. 2851
Main Authors: Wisse, Eddie, Braet, Filip, Duimel, Hans, Vreuls, Celien, Koek, Ger, Olde Damink, Steven W M, van den Broek, Maartje A J, De Geest, Bart, Dejong, Cees H C, Tateno, Chise, Frederik, Peter
Format: Journal Article
Language:English
Published: United States 21.06.2010
Subjects:
Animals
Biopsy
Cells, Cultured
Histocytological Preparation Techniques - methods
Humans
Liver - ultrastructure
Microscopy, Electron
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Perfusion
Tissue Fixation - methods
ISSN:2219-2840, 2219-2840
Online Access:Get more information
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Summary:For an electron microscopic study of the liver, expertise and complicated, time-consuming processing of hepatic tissues and cells is needed. The interpretation of electron microscopy (EM) images requires knowledge of the liver fine structure and experience with the numerous artifacts in fixation, embedding, sectioning, contrast staining and microscopic imaging. Hence, the aim of this paper is to present a detailed summary of different methods for the preparation of hepatic cells and tissue, for the purpose of preserving long-standing expertise and to encourage new investigators and clinicians to include EM studies of liver cells and tissue in their projects.
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ISSN:2219-2840
2219-2840
DOI:10.3748/wjg.v16.i23.2851