Fixation methods for electron microscopy of human and other liver

For an electron microscopic study of the liver, expertise and complicated, time-consuming processing of hepatic tissues and cells is needed. The interpretation of electron microscopy (EM) images requires knowledge of the liver fine structure and experience with the numerous artifacts in fixation, em...

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Vydané v:World journal of gastroenterology : WJG Ročník 16; číslo 23; s. 2851
Hlavní autori: Wisse, Eddie, Braet, Filip, Duimel, Hans, Vreuls, Celien, Koek, Ger, Olde Damink, Steven W M, van den Broek, Maartje A J, De Geest, Bart, Dejong, Cees H C, Tateno, Chise, Frederik, Peter
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: United States 21.06.2010
Predmet:
Animals
Biopsy
Cells, Cultured
Histocytological Preparation Techniques - methods
Humans
Liver - ultrastructure
Microscopy, Electron
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Perfusion
Tissue Fixation - methods
ISSN:2219-2840, 2219-2840
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Popis
Shrnutí:For an electron microscopic study of the liver, expertise and complicated, time-consuming processing of hepatic tissues and cells is needed. The interpretation of electron microscopy (EM) images requires knowledge of the liver fine structure and experience with the numerous artifacts in fixation, embedding, sectioning, contrast staining and microscopic imaging. Hence, the aim of this paper is to present a detailed summary of different methods for the preparation of hepatic cells and tissue, for the purpose of preserving long-standing expertise and to encourage new investigators and clinicians to include EM studies of liver cells and tissue in their projects.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
ObjectType-Review-3
content type line 23
ISSN:2219-2840
2219-2840
DOI:10.3748/wjg.v16.i23.2851