The Zucker Diabetic Fatty Rat as a Model for Vascular Changes in Diabetic Kidney Disease: Characterising Hydronephrosis

Background/Objectives: Diabetic kidney disease (DKD) is a significant concern for global healthcare, particularly in individuals with diabetes. The Zucker rat strain is a commonly used model of type 2 diabetes, despite awareness that this animal can develop hydronephrosis. In this study, we present...

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Vydáno v:Diagnostics (Basel) Ročník 15; číslo 6; s. 782
Hlavní autoři: McDermott, Amy, Panduro, Nathalie Sarup, Taghavi, Iman, Kjer, Hans Martin, Søgaard, Stinne Byrholdt, Nielsen, Michael Bachmann, Jensen, Jørgen Arendt, Sørensen, Charlotte Mehlin
Médium: Journal Article
Jazyk:angličtina
Vydáno: Switzerland MDPI AG 20.03.2025
MDPI
Témata:
Aldehydes
Animals
Carotid arteries
Catheters
Contrast agents
Creatinine
Denmark
Diabetes
diabetic kidney disease
Diagnostic imaging
Diseases
Glucose
Hydronephrosis
Hypotheses
Kidney diseases
Laboratories
Medical research
Medicine, Experimental
Mutation
Nitrogen
Ostomy
renal imaging
Research methodology
Type 2 diabetes
Veins & arteries
Zucker Diabetic Fatty rat
μCT
Denmark
United Kingdom > UK
United States > US
diabetic kidney disease
type 2 diabetes
μCT
renal imaging
hydronephrosis
Zucker Diabetic Fatty rat
ISSN:2075-4418, 2075-4418
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Shrnutí:Background/Objectives: Diabetic kidney disease (DKD) is a significant concern for global healthcare, particularly in individuals with diabetes. The Zucker rat strain is a commonly used model of type 2 diabetes, despite awareness that this animal can develop hydronephrosis. In this study, we present novel imaging data evaluating the accuracy of this animal model in replicating the vascular aspects of human DKD while examining the impact of hydronephrosis on its validity as a disease model. Methods: This study reused data from a population of male Zucker Diabetic Fatty (ZDF; n = 22) rats and Zucker Lean (ZL) rats (n = 22) aged 12 to approximately 40 weeks. Vascular casting was performed to enable visualisation of the renal vasculature. Anatomical regional volumes and vascular density data were obtained from μCT scans using image thresholding and manual analysis. The effects of hydronephrosis were evaluated using renal functional parameters and histological examination. Results: A significantly lower cortical vascular density, as well as lower total renal vascular density, was seen in ZDF rats compared to ZL rats, independent of age. We identified that hydronephrosis affected 92% of ZDF rats and 69% of ZL rats. Hydronephrosis cavity size was significantly correlated with the degree of hyperglycaemia and rate of diuresis but had no other detected impact on renal function, vascularity, or tissue histological architecture. Conclusions: These findings support using the Zucker rat strain as a model for vascular changes in DKD. Despite identifying severe hydronephrosis in this population, it had minimal quantifiable impact on renal function or diabetes modelling.
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SourceType-Scholarly Journals-1
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ISSN:2075-4418
2075-4418
DOI:10.3390/diagnostics15060782