Structures of Naturally Evolved CUP1 Tandem Arrays in Yeast Indicate That These Arrays Are Generated by Unequal Nonhomologous Recombination

Abstract An important issue in genome evolution is the mechanism by which tandem duplications are generated from single-copy genes. In the yeast Saccharomyces cerevisiae, most strains contain tandemly duplicated copies of CUP1, a gene that encodes a copper-binding metallothionein. By screening 101 n...

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Published in:G3 : genes - genomes - genetics Vol. 4; no. 11; pp. 2259 - 2269
Main Authors: Zhao, Ying, Strope, Pooja K, Kozmin, Stanislav G, McCusker, John H, Dietrich, Fred S, Kokoska, Robert J, Petes, Thomas D
Format: Journal Article
Language:English
Published: United States Oxford University Press 01.11.2014
Genetics Society of America
Subjects:
Evolution, Molecular
Gene Duplication
Homologous Recombination
Investigations
Metallothionein - genetics
Saccharomyces cerevisiae - genetics
copper resistance
recombination
tandemly repeated genes
Saccharomyces cerevisiae
ISSN:2160-1836, 2160-1836
Online Access:Get full text
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Summary:Abstract An important issue in genome evolution is the mechanism by which tandem duplications are generated from single-copy genes. In the yeast Saccharomyces cerevisiae, most strains contain tandemly duplicated copies of CUP1, a gene that encodes a copper-binding metallothionein. By screening 101 natural isolates of S. cerevisiae, we identified five different types of CUP1-containing repeats, as well as strains that only had one copy of CUP1. A comparison of the DNA sequences of these strains indicates that the CUP1 tandem arrays were generated by unequal nonhomologous recombination events from strains that had one CUP1 gene.
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ISSN:2160-1836
2160-1836
DOI:10.1534/g3.114.012922