'dirty' business: testing the limitations of terminal restriction fragment length polymorphism (TRFLP) analysis of soil fungi

Terminal restriction fragment length polymorphism (TRFLP) is an increasingly popular method in molecular ecology. However, several key limitations of this method have not been fully examined especially when used to study fungi. We investigated the impact of spore contamination, intracollection ribos...

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Veröffentlicht in:	Molecular ecology Jg. 15; H. 3; S. 873 - 882
Hauptverfasser:	Avis, Peter G, Dickie, Ian A, Mueller, Gregory M
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.03.2006 Blackwell Publishing Ltd
Schlagworte:	analysis Base Sequence community ecology Deoxyribonucleic acid DNA DNA Restriction Enzymes DNA, Fungal DNA, Fungal - analysis DNA, Fungal - genetics DNA, Ribosomal Spacer DNA, Ribosomal Spacer - genetics ectomycorrhizae Enzymes Fluorescence Fungi Fungi - genetics Genetic Variation genetics internal transcribed spacers ITS variation loci Molecular Sequence Data mycorrhizal fungi Polymerase Chain Reaction Polymorphism Polymorphism, Restriction Fragment Length restriction enzyme inefficiency restriction enzyme recognition loci restriction fragment length polymorphism ribosomal DNA Soil analysis soil ecology soil fungi Soil Microbiology Soils Spores terminal restriction fragment length polymorphism TRFLP
ISSN:	0962-1083, 1365-294X
Online-Zugang:	Volltext
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