Toxicological analysis of limonene reaction products using an in vitro exposure system

► Exposure to reaction products may produce adverse health effects. ► A combined approach may help to investigate the role of indoor contaminants. ► Additional development is necessary for these types of in vitro models. Epidemiological investigations suggest a link between exposure to indoor air ch...

Full description

Saved in:
Bibliographic Details
Published in:Toxicology in vitro Vol. 27; no. 2; pp. 721 - 730
Main Authors: Anderson, Stacey E., Khurshid, Shahana S., Meade, B. Jean, Lukomska, Ewa, Wells, J.R.
Format: Journal Article
Language:English
Published: England Elsevier Ltd 01.03.2013
Subjects:
adverse effects
air
Air Pollutants - chemistry
Air Pollutants - toxicity
Air Pollution, Indoor
Cell Line, Tumor
Cell Proliferation - drug effects
chemical concentration
Cyclohexenes - chemistry
Cyclohexenes - toxicity
cytokines
Cytokines - metabolism
epidemiological studies
epithelial cells
exposure duration
Humans
In Vitro Techniques
Indoor air
Limonene
Ozone
Ozone - chemistry
Ozone - toxicity
pollutants
Respiratory Mucosa - drug effects
Respiratory Mucosa - metabolism
Secondary oxidation products
Terpenes - chemistry
Terpenes - toxicity
MCP-1
Secondary oxidation products
A549
Ozone
VOC
IL-8
IL-6
TNF-α
GM-CSF
FBS
ROS
Limonene
GSH
Indoor air
ISSN:0887-2333, 1879-3177, 1879-3177
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:► Exposure to reaction products may produce adverse health effects. ► A combined approach may help to investigate the role of indoor contaminants. ► Additional development is necessary for these types of in vitro models. Epidemiological investigations suggest a link between exposure to indoor air chemicals and adverse health effects. Consumer products contain reactive chemicals which can form secondary pollutants which may contribute to these effects. The reaction of limonene and ozone is a well characterized example of this type of indoor air chemistry. The studies described here characterize an in vitro model using an epithelial cell line (A549) or differentiated epithelial tissue (MucilAir™). The model is used to investigate adverse effects following exposure to combinations of limonene and ozone. In A549 cells, exposure to both the parent compounds and reaction products resulted in alterations in inflammatory cytokine production. A one hour exposure to limonene+ozone resulted in decreased proliferation when compared to cells exposed to limonene alone. Repeated dose exposures of limonene or limonene+ozone were conducted on MucilAir™ tissue. No change in proliferation was observed but increases in cytokine production were observed for both the parent compounds and reaction products. Factors such as exposure duration, chemical concentration, and sampling time point were identified to influence result outcome. These findings suggest that exposure to reaction products may produce more severe effects compared to the parent compound.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ObjectType-Article-2
ObjectType-Feature-1
ISSN:0887-2333
1879-3177
1879-3177
DOI:10.1016/j.tiv.2012.11.017