Fluorescence labeling of a NaV1.7-targeted peptide for near-infrared nerve visualization

Background Accidental peripheral nerve injury during surgical intervention results in a broad spectrum of potentially debilitating side effects. Tissue distortion and poor visibility can significantly increase the risk of nerve injury with long-lasting consequences for the patient. We developed and...

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Published in:EJNMMI research Vol. 10; no. 1; pp. 49 - 8
Main Authors: Gonzales, Junior, Pirovano, Giacomo, Chow, Chun Yuen, de Souza Franca, Paula Demetrio, Carter, Lukas M., Klint, Julie K., Guru, Navjot, Lewis, Jason S., King, Glenn F., Reiner, Thomas
Format: Journal Article
Language:English
Published: Berlin/Heidelberg Springer Berlin Heidelberg 14.05.2020
Springer Nature B.V
SpringerOpen
Subjects:
Cardiac Imaging
Fluorescence
Hs1a-FL
Imaging
Intraoperative
Medicine
Medicine & Public Health
Muscles
Near-infrared imaging
Nerve imaging
Nuclear Medicine
Oncology
Original Research
Orthopedics
Peripheral nerves
Physicians
Radiology
Side effects
Surgery
Tracers
Visibility
Visualization
Intraoperative
Hs1a-FL
Nerve imaging
Near-infrared imaging
ISSN:2191-219X, 2191-219X
Online Access:Get full text
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Summary:Background Accidental peripheral nerve injury during surgical intervention results in a broad spectrum of potentially debilitating side effects. Tissue distortion and poor visibility can significantly increase the risk of nerve injury with long-lasting consequences for the patient. We developed and characterized Hs1a-FL, a fluorescent near-infrared molecule for nerve visualization in the operating theater with the aim of helping physicians to visualize nerves during surgery. Hs1a was derived from the venom of the Chinese bird spider, Haplopelma schmidti , and conjugated to Cy7.5 dye. Hs1a-FL was injected intravenously in mice, and harvested nerves were imaged microscopically and with epifluorescence. Results Hs1a-FL showed specific and stable binding to the sodium channel Na V 1.7, present on the surface of human and mouse nerves. Hs1a-FL allowed epifluorescence visualization of sciatic mouse nerves with favorable nerve-to-muscle contrast. Conclusions Fluorescent Na V 1.7-targeted tracers have the potential to be adopted clinically for the intraoperative visualization of peripheral nerves during surgery, providing guidance for the surgeon and potentially improving the standard of care.
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ISSN:2191-219X
2191-219X
DOI:10.1186/s13550-020-00630-4