One-step RT-PCR for detection of Zika virus

Zika virus (ZIKV) is an emerging mosquito-borne flavivirus circulating in Asia and Africa. Human infection induces an influenza-like syndrome that is associated with retro-orbital pain, oedema, lymphadenopathy, or diarrhea. Diagnosis of Zika fever requires virus isolation and serology, which are tim...

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Bibliographic Details
Published in:	Journal of clinical virology Vol. 43; no. 1; pp. 96 - 101
Main Authors:	Faye, Oumar, Faye, Ousmane, Dupressoir, Anne, Weidmann, Manfred, Ndiaye, Mady, Alpha Sall, Amadou
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 01.09.2008 Elsevier Science
Subjects:	Allergy and Immunology Biological and medical sciences Detection Diagnosis Flavivirus Fundamental and applied biological sciences. Psychology Genetic Variation Human viral diseases Infectious Disease Infectious diseases Medical sciences Microbiology Miscellaneous Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Viral - isolation & purification RT-PCR Sensitivity and Specificity Viral diseases Viral Envelope Proteins - genetics Virology Zika virus Zika Virus - genetics Zika Virus - isolation & purification Zika Virus Infection - diagnosis RNA DTT L-15 dNTPs Zika virus ZF ZIKV TE RT-PCR FBS CRORA Diagnosis Detection pfu WHO Collaborating Center for Arboviruses and Viral Hemorrhagic Fever ribonucleic acid plaque-forming unit dithiothreitol Zika fever fetal bovine serum deoxynucleotide triphosphates L-15 Leibovitz medium Tris–EDTA reverse transcriptase polymerase chain reaction Microbiology Reverse transcription polymerase chain reaction Virology
ISSN:	1386-6532, 1873-5967
Online Access:	Get full text
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