One-step RT-PCR for detection of Zika virus

Zika virus (ZIKV) is an emerging mosquito-borne flavivirus circulating in Asia and Africa. Human infection induces an influenza-like syndrome that is associated with retro-orbital pain, oedema, lymphadenopathy, or diarrhea. Diagnosis of Zika fever requires virus isolation and serology, which are tim...

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Vydáno v:	Journal of clinical virology Ročník 43; číslo 1; s. 96 - 101
Hlavní autoři:	Faye, Oumar, Faye, Ousmane, Dupressoir, Anne, Weidmann, Manfred, Ndiaye, Mady, Alpha Sall, Amadou
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	Amsterdam Elsevier B.V 01.09.2008 Elsevier Science
Témata:	Allergy and Immunology Biological and medical sciences Detection Diagnosis Flavivirus Fundamental and applied biological sciences. Psychology Genetic Variation Human viral diseases Infectious Disease Infectious diseases Medical sciences Microbiology Miscellaneous Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Viral - isolation & purification RT-PCR Sensitivity and Specificity Viral diseases Viral Envelope Proteins - genetics Virology Zika virus Zika Virus - genetics Zika Virus - isolation & purification Zika Virus Infection - diagnosis RNA DTT L-15 dNTPs Zika virus ZF ZIKV TE RT-PCR FBS CRORA Diagnosis Detection pfu WHO Collaborating Center for Arboviruses and Viral Hemorrhagic Fever ribonucleic acid plaque-forming unit dithiothreitol Zika fever fetal bovine serum deoxynucleotide triphosphates L-15 Leibovitz medium Tris–EDTA reverse transcriptase polymerase chain reaction Microbiology Reverse transcription polymerase chain reaction Virology
ISSN:	1386-6532, 1873-5967
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Abstract	Zika virus (ZIKV) is an emerging mosquito-borne flavivirus circulating in Asia and Africa. Human infection induces an influenza-like syndrome that is associated with retro-orbital pain, oedema, lymphadenopathy, or diarrhea. Diagnosis of Zika fever requires virus isolation and serology, which are time consuming or cross-reactive. To develop a one-step RT-PCR assay to detect ZIKV in human serum. An assay targeting the envelope protein coding region was designed and evaluated for its specificity, detection limit, repeatability, and capacity to detect ZIKV isolates collected over a 40-year period from various African countries and hosts. The assay's detection limit and repeatability were respectively 7.7 pfu/reaction and 100% in serum and L-15 medium; none of 19 other flaviviruses tested were detected. The assay is rapid, sensitive, and specific to detect ZIKV in cell culture or serum, but needs to be validated for diagnosis using clinical samples.
AbstractList	Zika virus (ZIKV) is an emerging mosquito-borne flavivirus circulating in Asia and Africa. Human infection induces an influenza-like syndrome that is associated with retro-orbital pain, oedema, lymphadenopathy, or diarrhea. Diagnosis of Zika fever requires virus isolation and serology, which are time consuming or cross-reactive.BACKGROUNDZika virus (ZIKV) is an emerging mosquito-borne flavivirus circulating in Asia and Africa. Human infection induces an influenza-like syndrome that is associated with retro-orbital pain, oedema, lymphadenopathy, or diarrhea. Diagnosis of Zika fever requires virus isolation and serology, which are time consuming or cross-reactive.To develop a one-step RT-PCR assay to detect ZIKV in human serum.OBJECTIVETo develop a one-step RT-PCR assay to detect ZIKV in human serum.An assay targeting the envelope protein coding region was designed and evaluated for its specificity, detection limit, repeatability, and capacity to detect ZIKV isolates collected over a 40-year period from various African countries and hosts.STUDY DESIGNAn assay targeting the envelope protein coding region was designed and evaluated for its specificity, detection limit, repeatability, and capacity to detect ZIKV isolates collected over a 40-year period from various African countries and hosts.The assay's detection limit and repeatability were respectively 7.7pfu/reaction and 100% in serum and L-15 medium; none of 19 other flaviviruses tested were detected.RESULTSThe assay's detection limit and repeatability were respectively 7.7pfu/reaction and 100% in serum and L-15 medium; none of 19 other flaviviruses tested were detected.The assay is rapid, sensitive, and specific to detect ZIKV in cell culture or serum, but needs to be validated for diagnosis using clinical samples.CONCLUSIONSThe assay is rapid, sensitive, and specific to detect ZIKV in cell culture or serum, but needs to be validated for diagnosis using clinical samples. Zika virus (ZIKV) is an emerging mosquito-borne flavivirus circulating in Asia and Africa. Human infection induces an influenza-like syndrome that is associated with retro-orbital pain, oedema, lymphadenopathy, or diarrhea. Diagnosis of Zika fever requires virus isolation and serology, which are time consuming or cross-reactive. To develop a one-step RT-PCR assay to detect ZIKV in human serum. An assay targeting the envelope protein coding region was designed and evaluated for its specificity, detection limit, repeatability, and capacity to detect ZIKV isolates collected over a 40-year period from various African countries and hosts. The assay's detection limit and repeatability were respectively 7.7pfu/reaction and 100% in serum and L-15 medium; none of 19 other flaviviruses tested were detected. The assay is rapid, sensitive, and specific to detect ZIKV in cell culture or serum, but needs to be validated for diagnosis using clinical samples. Zika virus (ZIKV) is an emerging mosquito-borne flavivirus circulating in Asia and Africa. Human infection induces an influenza-like syndrome that is associated with retro-orbital pain, oedema, lymphadenopathy, or diarrhea. Diagnosis of Zika fever requires virus isolation and serology, which are time consuming or cross-reactive. To develop a one-step RT-PCR assay to detect ZIKV in human serum. An assay targeting the envelope protein coding region was designed and evaluated for its specificity, detection limit, repeatability, and capacity to detect ZIKV isolates collected over a 40-year period from various African countries and hosts. The assay's detection limit and repeatability were respectively 7.7 pfu/reaction and 100% in serum and L-15 medium; none of 19 other flaviviruses tested were detected. The assay is rapid, sensitive, and specific to detect ZIKV in cell culture or serum, but needs to be validated for diagnosis using clinical samples. Background: Zika virus (ZIKV) is an emerging mosquito-borne flavivirus circulating in Asia and Africa. Human infection induces an influenza-like syndrome that is associated with retro-orbital pain, oedema, lymphadenopathy, or diarrhea. Diagnosis of Zika fever requires virus isolation and serology, which are time consuming or cross-reactive. Objective: To develop a one-step RT-PCR assay to detect ZIKV in human serum. Study design: An assay targeting the envelope protein coding region was designed and evaluated for its specificity, detection limit, repeatability, and capacity to detect ZIKV isolates collected over a 40-year period from various African countries and hosts. Results: The assay's detection limit and repeatability were respectively 7.7pfu/reaction and 100% in serum and L-15 medium; none of 19 other flaviviruses tested were detected. Conclusions: The assay is rapid, sensitive, and specific to detect ZIKV in cell culture or serum, but needs to be validated for diagnosis using clinical samples. Hemorrhagic Fever Abstract Background Zika virus (ZIKV) is an emerging mosquito-borne flavivirus circulating in Asia and Africa. Human infection induces an influenza-like syndrome that is associated with retro-orbital pain, oedema, lymphadenopathy, or diarrhea. Diagnosis of Zika fever requires virus isolation and serology, which are time consuming or cross-reactive. Objective To develop a one-step RT-PCR assay to detect ZIKV in human serum. Study design An assay targeting the envelope protein coding region was designed and evaluated for its specificity, detection limit, repeatability, and capacity to detect ZIKV isolates collected over a 40-year period from various African countries and hosts. Results The assay's detection limit and repeatability were respectively 7.7 pfu/reaction and 100% in serum and L-15 medium; none of 19 other flaviviruses tested were detected. Conclusions The assay is rapid, sensitive, and specific to detect ZIKV in cell culture or serum, but needs to be validated for diagnosis using clinical samples.
Author	Alpha Sall, Amadou Dupressoir, Anne Faye, Oumar Faye, Ousmane Ndiaye, Mady Weidmann, Manfred
Author_xml	– sequence: 1 givenname: Oumar surname: Faye fullname: Faye, Oumar organization: Institut Pasteur de Dakar, 36 Avenue Pasteur, BP 220, Dakar, Senegal – sequence: 2 givenname: Ousmane surname: Faye fullname: Faye, Ousmane organization: Institut Pasteur de Dakar, 36 Avenue Pasteur, BP 220, Dakar, Senegal – sequence: 3 givenname: Anne surname: Dupressoir fullname: Dupressoir, Anne organization: Institut Pasteur de Dakar, 36 Avenue Pasteur, BP 220, Dakar, Senegal – sequence: 4 givenname: Manfred surname: Weidmann fullname: Weidmann, Manfred organization: Institute of Virology, University of Göttingen, Kreuzbergring 57, 37075 Göttingen, Germany – sequence: 5 givenname: Mady surname: Ndiaye fullname: Ndiaye, Mady organization: University Cheikh Anta Diop Dakar, 24 Avenue Cheikh Anta Diop, Dakar, Senegal – sequence: 6 givenname: Amadou surname: Alpha Sall fullname: Alpha Sall, Amadou email: asall@pasteur.sn organization: Institut Pasteur de Dakar, 36 Avenue Pasteur, BP 220, Dakar, Senegal
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Cites_doi	10.1016/0035-9203(58)90085-3 10.1016/0035-9203(81)90100-0 10.1016/0035-9203(82)90161-4 10.1016/S0923-2516(07)80011-2 10.1016/0928-0197(94)90034-5 10.1017/S0022172400025997 10.1128/JCM.39.5.1922-1927.2001 10.1093/nar/25.24.4876 10.1128/JCM.29.10.2107-2110.1991 10.7589/0090-3558-39.1.73 10.1007/s00705-006-0903-z 10.1016/S0022-2836(05)80360-2 10.1016/0035-9203(52)90042-4 10.1016/0035-9203(64)90201-9 10.1016/S0923-2516(06)80135-4 10.1016/S0166-0934(98)00003-2 10.1016/0035-9203(54)90006-1
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Keywords	RNA DTT L-15 dNTPs Zika virus ZF ZIKV TE RT-PCR FBS CRORA Diagnosis Detection pfu WHO Collaborating Center for Arboviruses and Viral Hemorrhagic Fever ribonucleic acid plaque-forming unit dithiothreitol Zika fever fetal bovine serum deoxynucleotide triphosphates L-15 Leibovitz medium Tris–EDTA reverse transcriptase polymerase chain reaction Microbiology Reverse transcription polymerase chain reaction Virology
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References	Dick, Kitchen, Haddow (bib6) 1952; 46 Fagbami (bib8) 1979; 83 Robert, Lhuillier, Meunier, Sarthou, Monteny (bib20) 1986; 86 McCrae, Kirya (bib13) 1982; 76 Akoua-Koffi, Diarrasouba, Bénié, Nigbichi, Bozona (bib1) 2001; 94 Monlun, Zeller, Le Guenno, Traoré-Lamizana, Hervy (bib15) 1993; 86 Mondet B. Zika Virus Outbreak—Micoronesia (Yap). Promed. Archive number 20070702.2108 July 2, 2007. Cited 29 Jun 2007. Available from Olson, Ksiazek, Suhandiman, Triwibowo (bib18) 1981; 75 Thompson, Gibson, Plewniak, Jeanmougin, Higgins (bib23) 1997; 25 Digoutte, Calvo-Wilson, Mondo, Traore-Lamizana, Adam (bib7) 1992; 143 MacNamara (bib12) 1954; 48 Altschul, Gish, Miller, Myers, Lipman (bib2) 1990; 215 Simpson (bib22) 1964; 58 Bel M. Zika Virus Micronesia (Yap). Promed. Archive number 20070713.2252, August 7, 2007 [Cited 6 August 2007]. Available from Kilbourn, Karesh, Wolfe, Bosi, Cook, Andau (bib9) 2003; 39 Kuno, Chang (bib10) 2007; 152 Kuno (bib11) 1998; 72 Brown, Chang, Cropp, Robbins, Tsai (bib5) 1994; 2 . Scaramozzino, Crance, Jouan, Debriel, Stoll, Garin (bib21) 2001; 139 Pierre, Drouet, Deubel (bib19) 1994; 245 Weinbren, Williams (bib24) 1958; 52 Morita, Tanaka, Igarashi (bib16) 1991; 29 Nathan, Kilbourn, Karesh, Rahman, Bosi (bib17) 2001; 65 Bearcroft (bib3) 1956; 50 Robert (10.1016/j.jcv.2008.05.005_bib20) 1986; 86 Akoua-Koffi (10.1016/j.jcv.2008.05.005_bib1) 2001; 94 Altschul (10.1016/j.jcv.2008.05.005_bib2) 1990; 215 Fagbami (10.1016/j.jcv.2008.05.005_bib8) 1979; 83 Nathan (10.1016/j.jcv.2008.05.005_bib17) 2001; 65 Digoutte (10.1016/j.jcv.2008.05.005_bib7) 1992; 143 Kuno (10.1016/j.jcv.2008.05.005_bib11) 1998; 72 Morita (10.1016/j.jcv.2008.05.005_bib16) 1991; 29 Pierre (10.1016/j.jcv.2008.05.005_bib19) 1994; 245 Kilbourn (10.1016/j.jcv.2008.05.005_bib9) 2003; 39 McCrae (10.1016/j.jcv.2008.05.005_bib13) 1982; 76 Simpson (10.1016/j.jcv.2008.05.005_bib22) 1964; 58 10.1016/j.jcv.2008.05.005_bib14 Bearcroft (10.1016/j.jcv.2008.05.005_bib3) 1956; 50 10.1016/j.jcv.2008.05.005_bib4 Scaramozzino (10.1016/j.jcv.2008.05.005_bib21) 2001; 139 Thompson (10.1016/j.jcv.2008.05.005_bib23) 1997; 25 Weinbren (10.1016/j.jcv.2008.05.005_bib24) 1958; 52 Kuno (10.1016/j.jcv.2008.05.005_bib10) 2007; 152 Monlun (10.1016/j.jcv.2008.05.005_bib15) 1993; 86 MacNamara (10.1016/j.jcv.2008.05.005_bib12) 1954; 48 Dick (10.1016/j.jcv.2008.05.005_bib6) 1952; 46 Olson (10.1016/j.jcv.2008.05.005_bib18) 1981; 75 Brown (10.1016/j.jcv.2008.05.005_bib5) 1994; 2
References_xml	– volume: 2 start-page: 41 year: 1994 end-page: 51 ident: bib5 article-title: Detection of Yellow fever virus by polymerase chain reaction publication-title: Clin Diagn Virol – volume: 83 start-page: 213 year: 1979 end-page: 219 ident: bib8 article-title: Zika virus infection in Nigeria: virological and seroepidemiological investigation in Oyo State publication-title: J Hyg Camb – volume: 152 start-page: 687 year: 2007 end-page: 696 ident: bib10 article-title: Full-length sequencing and genomic characterization of Bagaza, Kedougou, and Zika viruses publication-title: Arch Virol – volume: 46 start-page: 506 year: 1952 end-page: 524 ident: bib6 article-title: Zika virus isolation and serological specificity publication-title: Trans Roy Soc Trop Med Hyg – volume: 72 start-page: 27 year: 1998 end-page: 41 ident: bib11 article-title: Universal diagnostic RT-PCR protocol for arbovirus publication-title: J Virol Method – volume: 58 start-page: 335 year: 1964 end-page: 338 ident: bib22 article-title: Zika virus infection in man publication-title: Trans Roy Soc Trop Med Hyg – reference: Bel M. Zika Virus Micronesia (Yap). Promed. Archive number 20070713.2252, August 7, 2007 [Cited 6 August 2007]. Available from – volume: 245 start-page: 93 year: 1994 end-page: 104 ident: bib19 article-title: Identification of mosquito-borne flavivirus sequences using universal primers and reverse transcription/polymerase chain reaction publication-title: Res Virol – volume: 143 start-page: 417 year: 1992 end-page: 422 ident: bib7 article-title: Continuous cell lines immune ascite fluid pools in arbovirus detection publication-title: Res Virol – volume: 139 start-page: 1922 year: 2001 end-page: 1927 ident: bib21 article-title: Comparison of flavivirus universal primer pairs and development of a rapid, highly sensitive heminested RT-PCR assay for detection of flaviviruses targeted to a conserved region of the NS5 gene sequence publication-title: J Clin Microbiol – volume: 86 start-page: 90 year: 1986 end-page: 100 ident: bib20 article-title: Virus Amaril, Dengue 2 et autres arbovirus isolés de moustiques, au Burkina Faso, de 1983 à 1986: considérations entomologiques et épidémiologiques publication-title: Bull Soc Path Ex – volume: 215 start-page: 403 year: 1990 end-page: 410 ident: bib2 article-title: Basic local alignment search tool publication-title: J Mol Biol – volume: 50 start-page: 48 year: 1956 end-page: 442 ident: bib3 article-title: Zika virus infection experimentally induced in a human volunteer publication-title: Trans Roy Soc Trop Med Hyg – volume: 86 start-page: 21 year: 1993 end-page: 28 ident: bib15 article-title: Surveillance de la circulation des arbovirus d’intérêt médical dans la région du Sénégal Oriental (1988–1991) publication-title: Bull Soc Path Ex – volume: 29 start-page: 2107 year: 1991 end-page: 2110 ident: bib16 article-title: Rapid identification of dengue virus serotypes by using polymerase chain reaction publication-title: J Clinic Microbiol – volume: 48 start-page: 139 year: 1954 end-page: 145 ident: bib12 article-title: Zika virus: a report on three cases of human infection during an epidemic of jaundice in Nigeria publication-title: Trans Roy Soc Trop Med Hyg – volume: 65 start-page: 310 year: 2001 end-page: 316 ident: bib17 article-title: Sylvatic transmission of Aboviruses among Bornean orangutans publication-title: Am J Trop Med Hyg – volume: 76 start-page: 552 year: 1982 end-page: 562 ident: bib13 article-title: Yellow fever and Zika virus epizootics and enzootics in Uganda publication-title: Trans Roy Soc Trop Med Hyg – volume: 25 start-page: 4876 year: 1997 end-page: 4882 ident: bib23 article-title: The ClustalX windowns interface: flexible strategies for multiple sequence alignment aided by quality analysis tools publication-title: Nucleic Acids Res – volume: 94 start-page: 227 year: 2001 end-page: 230 ident: bib1 article-title: Investigation autour d’un cas mortel de fièvre jaune en Côted’Ivoire en 1999 publication-title: Bull Soc Pathol Exot – volume: 75 start-page: 389 year: 1981 end-page: 393 ident: bib18 article-title: Zika virus, a cause of fever in Central Java, Indonesia publication-title: Trans Roy Soc Trop Med Hyg – volume: 39 year: 2003 ident: bib9 article-title: Health evaluation of free-ranging and semi-captive Ourangutans (Pongo Pygmaeus) in Sabah Malaysia publication-title: J Wildlife Dis – reference: Mondet B. Zika Virus Outbreak—Micoronesia (Yap). Promed. Archive number 20070702.2108 July 2, 2007. Cited 29 Jun 2007. Available from – volume: 52 start-page: 263 year: 1958 end-page: 268 ident: bib24 article-title: Zika virus: further isolations in the Zika area, and some studies on the strains isolated publication-title: Trans Roy Soc Trop Med Hyg – reference: . – ident: 10.1016/j.jcv.2008.05.005_bib14 – volume: 52 start-page: 263 year: 1958 ident: 10.1016/j.jcv.2008.05.005_bib24 article-title: Zika virus: further isolations in the Zika area, and some studies on the strains isolated publication-title: Trans Roy Soc Trop Med Hyg doi: 10.1016/0035-9203(58)90085-3 – volume: 75 start-page: 389 issue: 3 year: 1981 ident: 10.1016/j.jcv.2008.05.005_bib18 article-title: Zika virus, a cause of fever in Central Java, Indonesia publication-title: Trans Roy Soc Trop Med Hyg doi: 10.1016/0035-9203(81)90100-0 – volume: 76 start-page: 552 issue: 4 year: 1982 ident: 10.1016/j.jcv.2008.05.005_bib13 article-title: Yellow fever and Zika virus epizootics and enzootics in Uganda publication-title: Trans Roy Soc Trop Med Hyg doi: 10.1016/0035-9203(82)90161-4 – volume: 245 start-page: 93 year: 1994 ident: 10.1016/j.jcv.2008.05.005_bib19 article-title: Identification of mosquito-borne flavivirus sequences using universal primers and reverse transcription/polymerase chain reaction publication-title: Res Virol doi: 10.1016/S0923-2516(07)80011-2 – volume: 2 start-page: 41 year: 1994 ident: 10.1016/j.jcv.2008.05.005_bib5 article-title: Detection of Yellow fever virus by polymerase chain reaction publication-title: Clin Diagn Virol doi: 10.1016/0928-0197(94)90034-5 – ident: 10.1016/j.jcv.2008.05.005_bib4 – volume: 83 start-page: 213 year: 1979 ident: 10.1016/j.jcv.2008.05.005_bib8 article-title: Zika virus infection in Nigeria: virological and seroepidemiological investigation in Oyo State publication-title: J Hyg Camb doi: 10.1017/S0022172400025997 – volume: 139 start-page: 1922 issue: 5 year: 2001 ident: 10.1016/j.jcv.2008.05.005_bib21 article-title: Comparison of flavivirus universal primer pairs and development of a rapid, highly sensitive heminested RT-PCR assay for detection of flaviviruses targeted to a conserved region of the NS5 gene sequence publication-title: J Clin Microbiol doi: 10.1128/JCM.39.5.1922-1927.2001 – volume: 25 start-page: 4876 year: 1997 ident: 10.1016/j.jcv.2008.05.005_bib23 article-title: The ClustalX windowns interface: flexible strategies for multiple sequence alignment aided by quality analysis tools publication-title: Nucleic Acids Res doi: 10.1093/nar/25.24.4876 – volume: 29 start-page: 2107 issue: 10 year: 1991 ident: 10.1016/j.jcv.2008.05.005_bib16 article-title: Rapid identification of dengue virus serotypes by using polymerase chain reaction publication-title: J Clinic Microbiol doi: 10.1128/JCM.29.10.2107-2110.1991 – volume: 65 start-page: 310 issue: 5–6 year: 2001 ident: 10.1016/j.jcv.2008.05.005_bib17 article-title: Sylvatic transmission of Aboviruses among Bornean orangutans publication-title: Am J Trop Med Hyg – volume: 39 issue: 1 year: 2003 ident: 10.1016/j.jcv.2008.05.005_bib9 article-title: Health evaluation of free-ranging and semi-captive Ourangutans (Pongo Pygmaeus) in Sabah Malaysia publication-title: J Wildlife Dis doi: 10.7589/0090-3558-39.1.73 – volume: 50 start-page: 48 issue: 3 year: 1956 ident: 10.1016/j.jcv.2008.05.005_bib3 article-title: Zika virus infection experimentally induced in a human volunteer publication-title: Trans Roy Soc Trop Med Hyg – volume: 86 start-page: 90 year: 1986 ident: 10.1016/j.jcv.2008.05.005_bib20 article-title: Virus Amaril, Dengue 2 et autres arbovirus isolés de moustiques, au Burkina Faso, de 1983 à 1986: considérations entomologiques et épidémiologiques publication-title: Bull Soc Path Ex – volume: 94 start-page: 227 issue: 3 year: 2001 ident: 10.1016/j.jcv.2008.05.005_bib1 article-title: Investigation autour d’un cas mortel de fièvre jaune en Côted’Ivoire en 1999 publication-title: Bull Soc Pathol Exot – volume: 152 start-page: 687 year: 2007 ident: 10.1016/j.jcv.2008.05.005_bib10 article-title: Full-length sequencing and genomic characterization of Bagaza, Kedougou, and Zika viruses publication-title: Arch Virol doi: 10.1007/s00705-006-0903-z – volume: 215 start-page: 403 year: 1990 ident: 10.1016/j.jcv.2008.05.005_bib2 article-title: Basic local alignment search tool publication-title: J Mol Biol doi: 10.1016/S0022-2836(05)80360-2 – volume: 46 start-page: 506 year: 1952 ident: 10.1016/j.jcv.2008.05.005_bib6 article-title: Zika virus isolation and serological specificity publication-title: Trans Roy Soc Trop Med Hyg doi: 10.1016/0035-9203(52)90042-4 – volume: 86 start-page: 21 issue: 1 year: 1993 ident: 10.1016/j.jcv.2008.05.005_bib15 article-title: Surveillance de la circulation des arbovirus d’intérêt médical dans la région du Sénégal Oriental (1988–1991) publication-title: Bull Soc Path Ex – volume: 58 start-page: 335 year: 1964 ident: 10.1016/j.jcv.2008.05.005_bib22 article-title: Zika virus infection in man publication-title: Trans Roy Soc Trop Med Hyg doi: 10.1016/0035-9203(64)90201-9 – volume: 143 start-page: 417 year: 1992 ident: 10.1016/j.jcv.2008.05.005_bib7 article-title: Continuous cell lines immune ascite fluid pools in arbovirus detection publication-title: Res Virol doi: 10.1016/S0923-2516(06)80135-4 – volume: 72 start-page: 27 issue: 1/15 year: 1998 ident: 10.1016/j.jcv.2008.05.005_bib11 article-title: Universal diagnostic RT-PCR protocol for arbovirus publication-title: J Virol Method doi: 10.1016/S0166-0934(98)00003-2 – volume: 48 start-page: 139 issue: 2 year: 1954 ident: 10.1016/j.jcv.2008.05.005_bib12 article-title: Zika virus: a report on three cases of human infection during an epidemic of jaundice in Nigeria publication-title: Trans Roy Soc Trop Med Hyg doi: 10.1016/0035-9203(54)90006-1
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Snippet	Zika virus (ZIKV) is an emerging mosquito-borne flavivirus circulating in Asia and Africa. Human infection induces an influenza-like syndrome that is... Abstract Background Zika virus (ZIKV) is an emerging mosquito-borne flavivirus circulating in Asia and Africa. Human infection induces an influenza-like... Background: Zika virus (ZIKV) is an emerging mosquito-borne flavivirus circulating in Asia and Africa. Human infection induces an influenza-like syndrome that...
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SubjectTerms	Allergy and Immunology Biological and medical sciences Detection Diagnosis Flavivirus Fundamental and applied biological sciences. Psychology Genetic Variation Human viral diseases Infectious Disease Infectious diseases Medical sciences Microbiology Miscellaneous Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Viral - isolation & purification RT-PCR Sensitivity and Specificity Viral diseases Viral Envelope Proteins - genetics Virology Zika virus Zika Virus - genetics Zika Virus - isolation & purification Zika Virus Infection - diagnosis
Title	One-step RT-PCR for detection of Zika virus
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