Epigenetics Identifier screens reveal regulators of chromatin acylation and limited specificity of acylation antibodies

The collection of known posttranslational modifications (PTMs) has expanded rapidly with the identification of various non-acetyl histone lysine acylations, such as crotonylation, succinylation and butyrylation, yet their regulation is still not fully understood. Through an unbiased chromatin immuno...

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Vydáno v:Scientific reports Ročník 11; číslo 1; s. 12795 - 17
Hlavní autoři: Kollenstart, Leonie, van der Horst, Sophie C., Vreeken, Kees, Janssen, George M. C., Martino, Fabrizio, Vlaming, Hanneke, van Veelen, Peter A., van Leeuwen, Fred, van Attikum, Haico
Médium: Journal Article
Jazyk:angličtina
Vydáno: London Nature Publishing Group UK 17.06.2021
Nature Publishing Group
Nature Portfolio
Témata:
631/337/176
631/45/612/100/2285
631/45/612/1239
Acetylation
Acylation
Antibodies
Chromatin
Cross-reactivity
Epigenetics
Histones
Humanities and Social Sciences
Immunoprecipitation
Lysine
multidisciplinary
Science
Science (multidisciplinary)
Yeast
ISSN:2045-2322, 2045-2322
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Popis
Shrnutí:The collection of known posttranslational modifications (PTMs) has expanded rapidly with the identification of various non-acetyl histone lysine acylations, such as crotonylation, succinylation and butyrylation, yet their regulation is still not fully understood. Through an unbiased chromatin immunoprecipitation (ChIP)-based approach called Epigenetics-IDentifier (Epi-ID), we aimed to identify regulators of crotonylation, succinylation and butyrylation in thousands of yeast mutants simultaneously. However, highly correlative results led us to further investigate the specificity of the pan-K-acyl antibodies used in our Epi-ID studies. This revealed cross-reactivity and lack of specificity of pan-K-acyl antibodies in various assays. Our findings suggest that the antibodies might recognize histone acetylation in vivo, in addition to histone acylation, due to the vast overabundance of acetylation compared to other acylation modifications in cells. Consequently, our Epi-ID screen mostly identified factors affecting histone acetylation, including known (e.g. GCN5, HDA1 , and HDA2 ) and unanticipated ( MET7 , MTF1, CLB3, and RAD26 ) factors , expanding the repertoire of acetylation regulators. Antibody-independent follow-up experiments on the Gcn5-Ada2-Ada3 (ADA) complex revealed that, in addition to acetylation and crotonylation, ADA has the ability to butyrylate histones. Thus, our Epi-ID screens revealed limits of using pan-K-acyl antibodies in epigenetics research, expanded the repertoire of regulators of histone acetylation, and attributed butyrylation activity to the ADA complex.
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ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-91359-0