Recombination-induced tag exchange to track old and new proteins

The dynamic behavior of proteins is critical for cellular homeostasis. However, analyzing dynamics of proteins and protein complexes in vivo has been difficult. Here we describe recombination-induced tag exchange (RITE), a genetic method that induces a permanent epitope-tag switch in the coding sequ...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS Jg. 107; H. 1; S. 64
Hauptverfasser:	Verzijlbergen, Kitty F, Menendez-Benito, Victoria, van Welsem, Tibor, van Deventer, Sjoerd J, Lindstrom, Derek L, Ovaa, Huib, Neefjes, Jacques, Gottschling, Daniel E, van Leeuwen, Fred
Format:	Journal Article
Sprache:	Englisch
Veröffentlicht:	United States 05.01.2010
Schlagworte:	Chromatin - genetics Chromatin - metabolism Epitopes - genetics Fluorescent Dyes - metabolism Histones - genetics Histones - metabolism Integrases - genetics Integrases - metabolism Proteasome Endopeptidase Complex - metabolism Proteins - genetics Proteins - metabolism Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Recombination, Genetic - physiology Reproducibility of Results
ISSN:	1091-6490, 1091-6490
Online-Zugang:	Weitere Angaben
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