Model-based analysis of sample index hopping reveals its widespread artifacts in multiplexed single-cell RNA-sequencing

Index hopping is the main cause of incorrect sample assignment of sequencing reads in multiplexed pooled libraries. We introduce a statistical model for estimating the sample index-hopping rate in multiplexed droplet-based single-cell RNA-seq data and for probabilistic inference of the true sample o...

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Bibliographic Details
Published in:	Nature communications Vol. 11; no. 1; pp. 2704 - 8
Main Authors:	Farouni, Rick, Djambazian, Haig, Ferri, Lorenzo E., Ragoussis, Jiannis, Najafabadi, Hamed S.
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 01.06.2020 Nature Publishing Group Nature Portfolio
Subjects:	38/91 631/114/2415 631/208/514/1949 Datasets Droplets Gene expression Gene sequencing Humanities and Social Sciences Mathematical models multidisciplinary Multiplexing Probabilistic inference Purging Ribonucleic acid RNA Samples Science Science (multidisciplinary) Statistical analysis Statistical methods Statistical models
ISSN:	2041-1723, 2041-1723
Online Access:	Get full text
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