Maximizing RNA yield from archival renal tumors and optimizing gene expression analysis

Formalin-fixed, paraffin-embedded tissues are widely available for gene expression analysis using TaqMan PCR. Five methods, including 4 commercial kits, for recovering RNA from paraffin-embedded renal tumor tissue were compared. The MasterPure kit from Epicentre produced the highest RNA yield. Howev...

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Vydané v:	Journal of biomolecular screening Ročník 15; číslo 1; s. 80
Hlavní autori:	Glenn, Sean T, Head, Karen L, Teh, Bin T, Gross, Kenneth W, Kim, Hyung L
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	United States 01.01.2010
Predmet:	Biological Specimen Banks Gene Expression Profiling - methods Gene Expression Regulation, Neoplastic Kidney Neoplasms - genetics Microfluidic Analytical Techniques Polymerase Chain Reaction - instrumentation Reagent Kits, Diagnostic Reverse Transcription - genetics RNA - metabolism RNA, Neoplasm - isolation & purification Taq Polymerase - metabolism
ISSN:	1552-454X, 1552-454X
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