Identification of biomarkers for tuberculosis disease using a novel dual-color RT–MLPA assay

Owing to our lack of understanding of the factors that constitute protective immunity during natural infection with Mycobacterium tuberculosis ( Mtb ), there is an urgent need to identify host biomarkers that predict long-term outcome of infection in the absence of therapy. Moreover, the identificat...

Celý popis

Uloženo v:

Podrobná bibliografie
Vydáno v:	Genes and immunity Ročník 13; číslo 1; s. 71 - 82
Hlavní autoři:	Joosten, S A, Goeman, J J, Sutherland, J S, Opmeer, L, de Boer, K G, Jacobsen, M, Kaufmann, S H E, Finos, L, Magis-Escurra, C, Ota, M O C, Ottenhoff, T H M, Haks, M C
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	London Nature Publishing Group UK 01.01.2012 Nature Publishing Group
Témata:	Adaptive immunity Apoptosis Biological markers Biomarkers Biomedical and Life Sciences Biomedicine Cancer Research Cohort analysis Diagnosis Gene Expression Gene Expression Profiling Genes Genetic Markers - genetics Human Genetics Humans Identification Immune response Immune system Immunology Infections Infectious diseases Latent infection Lymphocytes B Lymphocytes T Mycobacterium tuberculosis Nucleic Acid Amplification Techniques - methods original-article Physiological aspects Polymerase chain reaction Real-Time Polymerase Chain Reaction - methods Reproducibility of Results Sensitivity and Specificity Transcriptomics Tuberculosis Tuberculosis - genetics Tuberculosis - immunology Vaccines Netherlands Gambia dcRT–MLPA tuberculosis host biomarkers
ISSN:	1466-4879, 1476-5470, 1476-5470
On-line přístup:	Získat plný text
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!

Abstract	Owing to our lack of understanding of the factors that constitute protective immunity during natural infection with Mycobacterium tuberculosis ( Mtb ), there is an urgent need to identify host biomarkers that predict long-term outcome of infection in the absence of therapy. Moreover, the identification of host biomarkers that predict (in)adequate response to tuberculosis (TB) treatment would similarly be a major step forward. To identify/monitor multi-component host biomarker signatures at the transcriptomic level in large human cohort studies, we have developed and validated a dual-color reverse-transcriptase multiplex ligation-dependent probe amplification (dcRT–MLPA) method, permitting rapid and accurate expression profiling of as many as 60–80 transcripts in a single reaction. dcRT–MLPA is sensitive, highly reproducible, high-throughput, has an extensive dynamic range and is as quantitative as QPCR. We have used dcRT–MLPA to characterize the human immune response to Mtb in several cohort studies in two genetically and geographically diverse populations. A biomarker signature was identified that is strongly associated with active TB disease, and was profoundly distinct from that associated with treated TB disease, latent infection or uninfected controls, demonstrating the discriminating power of our biomarker signature. Identified biomarkers included apoptosis-related genes and T-cell/B-cell markers, suggesting important contributions of adaptive immunity to TB pathogenesis.
AbstractList	Owing to our lack of understanding of the factors that constitute protective immunity during natural infection with Mycobacterium tuberculosis ( Mtb ), there is an urgent need to identify host biomarkers that predict long-term outcome of infection in the absence of therapy. Moreover, the identification of host biomarkers that predict (in)adequate response to tuberculosis (TB) treatment would similarly be a major step forward. To identify/monitor multi-component host biomarker signatures at the transcriptomic level in large human cohort studies, we have developed and validated a dual-color reverse-transcriptase multiplex ligation-dependent probe amplification (dcRT–MLPA) method, permitting rapid and accurate expression profiling of as many as 60–80 transcripts in a single reaction. dcRT–MLPA is sensitive, highly reproducible, high-throughput, has an extensive dynamic range and is as quantitative as QPCR. We have used dcRT–MLPA to characterize the human immune response to Mtb in several cohort studies in two genetically and geographically diverse populations. A biomarker signature was identified that is strongly associated with active TB disease, and was profoundly distinct from that associated with treated TB disease, latent infection or uninfected controls, demonstrating the discriminating power of our biomarker signature. Identified biomarkers included apoptosis-related genes and T-cell/B-cell markers, suggesting important contributions of adaptive immunity to TB pathogenesis. Owing to our lack of understanding of the factors that constitute protective immunity during natural infection with Mycobacterium tuberculosis (Mtb), there is an urgent need to identify host biomarkers that predict long-term outcome of infection in the absence of therapy. Moreover, the identification of host biomarkers that predict (in)adequate response to tuberculosis (TB) treatment would similarly be a major step forward. To identify/monitor multi-component host biomarker signatures at the transcriptomic level in large human cohort studies, we have developed and validated a dual-color reverse-transcriptase multiplex ligation-dependent probe amplification (dcRT-MLPA) method, permitting rapid and accurate expression profiling of as many as 60-80 transcripts in a single reaction. dcRT-MLPA is sensitive, highly reproducible, high- throughput, has an extensive dynamic range and is as quantitative as QPCR. We have used dcRT-MLPA to characterize the human immune response to Mtb in several cohort studies in two genetically and geographically diverse populations. A biomarker signature was identified that is strongly associated with active TB disease, and was profoundly distinct from that associated with treated TB disease, latent infection or uninfected controls, demonstrating the discriminating power of our biomarker signature. Identified biomarkers included apoptosis-related genes and T-cell/B-cell markers, suggesting important contributions of adaptive immunity to TB pathogenesis. Owing to our lack of understanding of the factors that constitute protective immunity during natural infection with Mycobacterium tuberculosis (Mtb), there is an urgent need to identify host biomarkers that predict long-term outcome of infection in the absence of therapy. Moreover, the identification of host biomarkers that predict (in)adequate response to tuberculosis (TB) treatment would similarly be a major step forward. To identify/monitor multi-component host biomarker signatures at the transcriptomic level in large human cohort studies, we have developed and validated a dual-color reverse-transcriptase multiplex ligation-dependent probe amplification (dcRT-MLPA) method, permitting rapid and accurate expression profiling of as many as 60-80 transcripts in a single reaction. dcRT-MLPA is sensitive, highly reproducible, high- throughput, has an extensive dynamic range and is as quantitative as QPCR. We have used dcRT-MLPA to characterize the human immune response to Mtb in several cohort studies in two genetically and geographically diverse populations. A biomarker signature was identified that is strongly associated with active TB disease, and was profoundly distinct from that associated with treated TB disease, latent infection or uninfected controls, demonstrating the discriminating power of our biomarker signature. Identified biomarkers included apoptosis-related genes and T-cell/B-cell markers, suggesting important contributions of adaptive immunity to TB pathogenesis. Genes and Immunity (2012) 13, 71-82; doi: 10.1038/gene.2011.64; published online 29 September 2011 Keywords: dcRT-MLPA; host biomarkers; tuberculosis Owing to our lack of understanding of the factors that constitute protective immunity during natural infection with Mycobacterium tuberculosis (Mtb), there is an urgent need to identify host biomarkers that predict long-term outcome of infection in the absence of therapy. Moreover, the identification of host biomarkers that predict (in)adequate response to tuberculosis (TB) treatment would similarly be a major step forward. To identify/monitor multi-component host biomarker signatures at the transcriptomic level in large human cohort studies, we have developed and validated a dual-color reverse-transcriptase multiplex ligation-dependent probe amplification (dcRT-MLPA) method, permitting rapid and accurate expression profiling of as many as 60-80 transcripts in a single reaction. dcRT-MLPA is sensitive, highly reproducible, high-throughput, has an extensive dynamic range and is as quantitative as QPCR. We have used dcRT-MLPA to characterize the human immune response to Mtb in several cohort studies in two genetically and geographically diverse populations. A biomarker signature was identified that is strongly associated with active TB disease, and was profoundly distinct from that associated with treated TB disease, latent infection or uninfected controls, demonstrating the discriminating power of our biomarker signature. Identified biomarkers included apoptosis-related genes and T-cell/B-cell markers, suggesting important contributions of adaptive immunity to TB pathogenesis.Owing to our lack of understanding of the factors that constitute protective immunity during natural infection with Mycobacterium tuberculosis (Mtb), there is an urgent need to identify host biomarkers that predict long-term outcome of infection in the absence of therapy. Moreover, the identification of host biomarkers that predict (in)adequate response to tuberculosis (TB) treatment would similarly be a major step forward. To identify/monitor multi-component host biomarker signatures at the transcriptomic level in large human cohort studies, we have developed and validated a dual-color reverse-transcriptase multiplex ligation-dependent probe amplification (dcRT-MLPA) method, permitting rapid and accurate expression profiling of as many as 60-80 transcripts in a single reaction. dcRT-MLPA is sensitive, highly reproducible, high-throughput, has an extensive dynamic range and is as quantitative as QPCR. We have used dcRT-MLPA to characterize the human immune response to Mtb in several cohort studies in two genetically and geographically diverse populations. A biomarker signature was identified that is strongly associated with active TB disease, and was profoundly distinct from that associated with treated TB disease, latent infection or uninfected controls, demonstrating the discriminating power of our biomarker signature. Identified biomarkers included apoptosis-related genes and T-cell/B-cell markers, suggesting important contributions of adaptive immunity to TB pathogenesis.
Audience	Academic
Author	Goeman, J J Magis-Escurra, C Sutherland, J S Opmeer, L Jacobsen, M Kaufmann, S H E Ota, M O C de Boer, K G Ottenhoff, T H M Joosten, S A Haks, M C Finos, L
Author_xml	– sequence: 1 givenname: S A surname: Joosten fullname: Joosten, S A organization: Department of Infectious Diseases, Leiden University Medical Center – sequence: 2 givenname: J J surname: Goeman fullname: Goeman, J J organization: Department of Medical Statistics and Bioinformatics, Leiden University Medical Center – sequence: 3 givenname: J S surname: Sutherland fullname: Sutherland, J S organization: Bacterial Diseases Programme, Medical Research Council Laboratories – sequence: 4 givenname: L surname: Opmeer fullname: Opmeer, L organization: Department of Infectious Diseases, Leiden University Medical Center – sequence: 5 givenname: K G surname: de Boer fullname: de Boer, K G organization: Department of Infectious Diseases, Leiden University Medical Center – sequence: 6 givenname: M surname: Jacobsen fullname: Jacobsen, M organization: Department of Immunology, Max Planck Institute for Infection Biology, 5Current address: Department of Immunology, Bernhard-Nocht-Institute for Tropical Medicine, Hamburg, Germany – sequence: 7 givenname: S H E surname: Kaufmann fullname: Kaufmann, S H E organization: Department of Immunology, Max Planck Institute for Infection Biology – sequence: 8 givenname: L surname: Finos fullname: Finos, L organization: Department of Medical Statistics and Bioinformatics, Leiden University Medical Center – sequence: 9 givenname: C surname: Magis-Escurra fullname: Magis-Escurra, C organization: Department of Infectious Diseases, Leiden University Medical Center, 6Current address: Department of Pulmonary Diseases, Nijmegen University Medical Center Dekkerswald, Groesbeek, The Netherlands – sequence: 10 givenname: M O C surname: Ota fullname: Ota, M O C organization: Bacterial Diseases Programme, Medical Research Council Laboratories – sequence: 11 givenname: T H M surname: Ottenhoff fullname: Ottenhoff, T H M organization: Department of Infectious Diseases, Leiden University Medical Center – sequence: 12 givenname: M C surname: Haks fullname: Haks, M C email: m.c.haks@lumc.nl organization: Department of Infectious Diseases, Leiden University Medical Center
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/21956656$$D View this record in MEDLINE/PubMed
BookMark	eNqF0stu1DAUBmALFdEL7FgjCxYIiQy2E1-yHFVcRhoEKmWL5TgnkUvGbu0E0R3vwBvyJDhMq2qqVsiLWNZ3ju34P0R7PnhA6CklC0pK9aYHDwtGKF2I6gE6oJUUBa8k2ZvnQhSVkvU-OkzpjBAqqKgfoX1Gay4EFwfo26oFP7rOWTO64HHocOPCxsTvEBPuQsTj1EC00xCSS7h1CUwCPCXne2ywDz9gwO1khsKGIeuT0z-_fn9cf15ik5K5fIwedmZI8OTqe4S-vnt7evyhWH96vzpergvLqRgLWhsqmrpRUBNQUHJiBSPQQScbAqW0NZFVVbG25KppQFHbSGuoURYY67goj9DLbd_zGC4mSKPeuGRhGIyHMCVdC0VFvjz_v6SS8rzX3PP5LXkWpujzNTISSnIlWUYv7kNMVFSWoq75jerNANr5LozR2HljvWRSiZIyrrJa3KHyaGHjbH71zuX1nYJXOwXZjPBz7M2Ukl59Odm1z64OOjUbaPV5dPmVL_V1FDJgW2BjSClCp60b_4Uin8INmhI9503PedNz3rSoctHrW0XXfe_hxZanzHwP8eZv3en_Apj_4xc
CitedBy_id	crossref_primary_10_1371_journal_pone_0073230 crossref_primary_10_1038_s41598_019_54213_y crossref_primary_10_1155_2013_851452 crossref_primary_10_1186_1471_2334_14_257 crossref_primary_10_3389_fmicb_2019_02789 crossref_primary_10_1002_ctm2_1375 crossref_primary_10_1016_j_ebiom_2020_102909 crossref_primary_10_2217_bmm_15_16 crossref_primary_10_3390_vaccines10060831 crossref_primary_10_1080_07853890_2024_2380797 crossref_primary_10_1093_infdis_jiv238 crossref_primary_10_1111_imr_12269 crossref_primary_10_1016_j_ebiom_2022_104173 crossref_primary_10_1186_1471_2334_13_456 crossref_primary_10_1016_j_ebiom_2014_12_001 crossref_primary_10_1111_imm_13012 crossref_primary_10_1371_journal_pntd_0004701 crossref_primary_10_1016_j_molimm_2022_07_008 crossref_primary_10_4049_jimmunol_1601138 crossref_primary_10_1093_jac_dkz207 crossref_primary_10_1186_s13075_020_02361_2 crossref_primary_10_1093_rheumatology_kead238 crossref_primary_10_1080_14789450_2022_2131545 crossref_primary_10_1111_1469_0691_12383 crossref_primary_10_3389_fimmu_2021_640168 crossref_primary_10_1183_13993003_01012_2016 crossref_primary_10_3389_fimmu_2020_00630 crossref_primary_10_14814_phy2_12394 crossref_primary_10_2217_bmm_13_53 crossref_primary_10_1038_gene_2015_21 crossref_primary_10_1093_infdis_jis499 crossref_primary_10_1038_srep38841 crossref_primary_10_1016_j_ebiom_2015_01_009 crossref_primary_10_1371_journal_pone_0226137 crossref_primary_10_1371_journal_pntd_0003243 crossref_primary_10_1186_s12935_025_03663_8 crossref_primary_10_1371_journal_pntd_0004331 crossref_primary_10_1038_gene_2014_13 crossref_primary_10_1016_j_tube_2013_11_003 crossref_primary_10_1111_iji_12340 crossref_primary_10_1016_j_tim_2012_06_002 crossref_primary_10_3389_fimmu_2020_626049 crossref_primary_10_1038_s41598_017_05057_x crossref_primary_10_1126_scitranslmed_aaj1701 crossref_primary_10_1371_journal_pone_0237574 crossref_primary_10_1080_14787210_2017_1247692 crossref_primary_10_1038_srep11295 crossref_primary_10_1093_infdis_jiy404 crossref_primary_10_1177_11772719221099130 crossref_primary_10_1016_j_vaccine_2014_06_014 crossref_primary_10_1002_advs_202301630 crossref_primary_10_1016_j_molimm_2022_03_004 crossref_primary_10_2147_IDR_S295798 crossref_primary_10_1016_j_vaccine_2015_04_054 crossref_primary_10_1038_s41598_022_14305_8 crossref_primary_10_1007_s10875_013_9979_x crossref_primary_10_1371_journal_pone_0106796 crossref_primary_10_1371_journal_ppat_1002607 crossref_primary_10_1016_j_micpath_2017_02_012 crossref_primary_10_1186_2193_1801_3_388 crossref_primary_10_1038_srep18520 crossref_primary_10_1016_j_cca_2025_120327 crossref_primary_10_1038_s41467_018_04579_w crossref_primary_10_1016_j_jtherbio_2022_103259 crossref_primary_10_4081_idr_2016_6568 crossref_primary_10_3389_fimmu_2018_01035 crossref_primary_10_1371_journal_pmed_1001538 crossref_primary_10_1517_17530059_2013_786039 crossref_primary_10_1016_j_tube_2016_06_003 crossref_primary_10_1038_gene_2013_26 crossref_primary_10_1371_journal_ppat_1004671 crossref_primary_10_3389_fimmu_2024_1350593 crossref_primary_10_3389_fimmu_2017_01563
Cites_doi	10.1111/j.1365-2249.2009.03967.x 10.1093/nar/gnf056 10.1038/nrmicro1126 10.1128/IAI.72.5.2628-2634.2004 10.1111/j.1469-0691.2005.01229.x 10.1007/s00109-007-0157-6 10.1111/j.1365-2249.2007.03399.x 10.1002/1521-4141(200206)32:6<1605::AID-IMMU1605>3.0.CO;2-6 10.1126/science.1155209 10.1146/annurev.immunol.021908.132703 10.1214/10-AOS829 10.1038/gene.2010.51 10.1016/j.immuni.2010.09.015 10.1093/nar/gng153 10.1002/humu.20054 10.1084/jem.20091885 10.1016/S0140-6736(10)60483-7 10.1002/eji.200939856 10.1093/biomet/asn007 10.1067/mcp.2001.113989 10.1086/510397 10.4049/jimmunol.172.11.6938 10.1038/nature09247 10.1038/4455 10.1002/9780470316856 10.1111/j.2517-6161.1996.tb02080.x
ContentType	Journal Article
Copyright	Macmillan Publishers Limited 2012 COPYRIGHT 2012 Nature Publishing Group Macmillan Publishers Limited 2012. Copyright Nature Publishing Group Jan 2012
Copyright_xml	– notice: Macmillan Publishers Limited 2012 – notice: COPYRIGHT 2012 Nature Publishing Group – notice: Macmillan Publishers Limited 2012. – notice: Copyright Nature Publishing Group Jan 2012
DBID	AAYXX CITATION CGR CUY CVF ECM EIF NPM ISR 3V. 7T5 7X7 7XB 88A 88E 8AO 8C1 8FD 8FE 8FH 8FI 8FJ 8FK ABUWG AFKRA AZQEC BBNVY BENPR BHPHI CCPQU DWQXO FR3 FYUFA GHDGH GNUQQ H94 HCIFZ K9. LK8 M0S M1P M7P P64 PHGZM PHGZT PJZUB PKEHL PPXIY PQEST PQGLB PQQKQ PQUKI PRINS RC3 7X8 7QL C1K
DOI	10.1038/gene.2011.64
DatabaseName	CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed Gale In Context: Science ProQuest Central (Corporate) Immunology Abstracts Health & Medical Collection ProQuest Central (purchase pre-March 2016) Biology Database (Alumni Edition) Medical Database (Alumni Edition) ProQuest Pharma Collection Public Health Database Technology Research Database ProQuest SciTech Collection ProQuest Natural Science Collection Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Central (Alumni Edition) ProQuest Central UK/Ireland ProQuest Central Essentials Biological Science Collection ProQuest Central Natural Science Collection ProQuest One Community College ProQuest Central Korea Engineering Research Database Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student AIDS and Cancer Research Abstracts SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) ProQuest Biological Science Collection Health & Medical Collection (Alumni Edition) Medical Database Biological Science Database Biotechnology and BioEngineering Abstracts ProQuest Central Premium ProQuest One Academic (New) ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic (retired) ProQuest One Academic UKI Edition ProQuest Central China Genetics Abstracts MEDLINE - Academic Bacteriology Abstracts (Microbiology B) Environmental Sciences and Pollution Management
DatabaseTitle	CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) ProQuest Central Student Technology Research Database ProQuest One Academic Middle East (New) ProQuest Central Essentials ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College ProQuest One Health & Nursing ProQuest Natural Science Collection ProQuest Pharma Collection ProQuest Central China ProQuest Biology Journals (Alumni Edition) ProQuest Central ProQuest One Applied & Life Sciences ProQuest Health & Medical Research Collection Genetics Abstracts Health Research Premium Collection Health and Medicine Complete (Alumni Edition) Natural Science Collection ProQuest Central Korea Health & Medical Research Collection Biological Science Collection AIDS and Cancer Research Abstracts ProQuest Central (New) ProQuest Medical Library (Alumni) ProQuest Public Health ProQuest Biological Science Collection ProQuest One Academic Eastern Edition ProQuest Hospital Collection Health Research Premium Collection (Alumni) Biological Science Database ProQuest SciTech Collection ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts ProQuest Health & Medical Complete ProQuest Medical Library ProQuest One Academic UKI Edition Immunology Abstracts Engineering Research Database ProQuest One Academic ProQuest One Academic (New) ProQuest Central (Alumni) MEDLINE - Academic Bacteriology Abstracts (Microbiology B) Environmental Sciences and Pollution Management
DatabaseTitleList	MEDLINE Genetics Abstracts ProQuest Central Student MEDLINE - Academic ProQuest Central Student
Database_xml	– sequence: 1 dbid: NPM name: PubMed url: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: BENPR name: ProQuest Central url: https://www.proquest.com/central sourceTypes: Aggregation Database
DeliveryMethod	fulltext_linktorsrc
Discipline	Biology
EISSN	1476-5470
EndPage	82
ExternalDocumentID	2565235861 A278631258 21956656 10_1038_gene_2011_64
Genre	Research Support, Non-U.S. Gov't Journal Article
GeographicLocations	Netherlands Gambia
GeographicLocations_xml	– name: Netherlands – name: Gambia
GrantInformation_xml	– fundername: Medical Research Council grantid: MC_UP_A900_1122 – fundername: Medical Research Council grantid: MC_U190071468
GroupedDBID	--- -Q- 0R~ 29H 2WC 36B 39C 4.4 406 53G 5GY 5RE 70F 7X7 88E 8AO 8C1 8FE 8FH 8FI 8FJ 8R4 8R5 AACDK AANZL AASML AATNV AAYZH ABAKF ABAWZ ABBRH ABDBE ABDBF ABFSG ABJNI ABLJU ABRTQ ABUWG ABZZP ACAOD ACGFS ACKTT ACPRK ACRQY ACSTC ACUHS ACZOJ ADBBV AEFQL AEJRE AEMSY AENEX AEVLU AEXYK AEZWR AFBBN AFDZB AFHIU AFKRA AFSHS AGAYW AGHAI AGQEE AHMBA AHSBF AHWEU AIGIU AILAN AIXLP AJRNO ALFFA ALIPV ALMA_UNASSIGNED_HOLDINGS AMYLF ATHPR AXYYD AYFIA B0M BAWUL BBNVY BENPR BHPHI BKKNO BPHCQ BVXVI CAG CCPQU COF CS3 DIK DNIVK DPUIP DU5 E3Z EAD EAP EBC EBD EBLON EBS EE. EIOEI EJD EMB EMK EMOBN EPL ESX F5P FDQFY FERAY FIGPU FIZPM FSGXE FYUFA HCIFZ HMCUK HZ~ IAO IHR INH INR ISR ITC IWAJR JSO JZLTJ KQ8 LK8 M1P M7P NQJWS O9- OK1 OVD P2P PHGZM PHGZT PJZUB PPXIY PQGLB PQQKQ PROAC PSQYO Q2X RNS RNT RNTTT ROL SNX SNYQT SOHCF SOJ SRMVM SV3 SWTZT TAOOD TBHMF TDRGL TEORI TR2 TSG TUS UKHRP Y6R ~8M AAYXX AFFHD CITATION CGR CUY CVF ECM EIF NPM 3V. 7T5 7XB 88A 8FD 8FK AZQEC DWQXO FR3 GNUQQ H94 K9. P64 PKEHL PQEST PQUKI PRINS RC3 7X8 PUEGO 7QL C1K
ID	FETCH-LOGICAL-c516t-19a16b9b8e90e8e350c620efef7b0e37c9074442d358bbe81cb7ca1a8ce22f563
IEDL.DBID	M7P
ISICitedReferencesCount	86
ISICitedReferencesURI	http://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=Summon&SrcAuth=ProQuest&DestLinkType=CitingArticles&DestApp=WOS_CPL&KeyUT=000299596900009&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
ISSN	1466-4879 1476-5470
IngestDate	Tue Oct 07 07:53:05 EDT 2025 Thu Oct 02 15:21:12 EDT 2025 Mon Oct 06 18:18:58 EDT 2025 Mon Oct 06 18:32:19 EDT 2025 Sat Nov 29 13:22:16 EST 2025 Sat Nov 29 10:09:14 EST 2025 Wed Nov 26 10:04:07 EST 2025 Mon Jul 21 06:03:08 EDT 2025 Sat Nov 29 08:10:14 EST 2025 Tue Nov 18 21:03:50 EST 2025 Mon Jul 21 06:08:38 EDT 2025
IsPeerReviewed	true
IsScholarly	true
Issue	1
Keywords	dcRT–MLPA tuberculosis host biomarkers
Language	English
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c516t-19a16b9b8e90e8e350c620efef7b0e37c9074442d358bbe81cb7ca1a8ce22f563
Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
PMID	21956656
PQID	2641736995
PQPubID	42724
PageCount	12
ParticipantIDs	proquest_miscellaneous_968166165 proquest_miscellaneous_917154446 proquest_journals_916875872 proquest_journals_2641736995 gale_infotracmisc_A278631258 gale_infotracacademiconefile_A278631258 gale_incontextgauss_ISR_A278631258 pubmed_primary_21956656 crossref_citationtrail_10_1038_gene_2011_64 crossref_primary_10_1038_gene_2011_64 springer_journals_10_1038_gene_2011_64
PublicationCentury	2000
PublicationDate	2012-01-01
PublicationDateYYYYMMDD	2012-01-01
PublicationDate_xml	– month: 01 year: 2012 text: 2012-01-01 day: 01
PublicationDecade	2010
PublicationPlace	London
PublicationPlace_xml	– name: London – name: England – name: Hamilton
PublicationTitle	Genes and immunity
PublicationTitleAbbrev	Genes Immun
PublicationTitleAlternate	Genes Immun
PublicationYear	2012
Publisher	Nature Publishing Group UK Nature Publishing Group
Publisher_xml	– name: Nature Publishing Group UK – name: Nature Publishing Group
References	Tibshirani (CR15) 1996; 58 White, Vink, Kriek, Wuyts, Schouten, Bakker (CR8) 2004; 24 Lienhardt, Azzurri, Amedei, Fielding, Sillah, Sow (CR12) 2002; 32 Burl, Hill, Jeffries, Holland, Fox, Lugos (CR22) 2007; 149 Bonecini-Almeida, Ho, Boechat, Huard, Chitale, Doo (CR9) 2004; 72 Knudsen, Gustafson, Kronborg, Kristiansen, Moestrup, Nielsen (CR11) 2005; 11 Maertzdorf, Repsilber, Parida, Stanley, Roberts, Black (CR19) 2011; 12 Meinshausen (CR27) 2008; 95 Goeman, Solari (CR26) 2010; 38 Jenner, Young (CR17) 2005; 3 Abebe, Doherty, Wassie, Aseffa, Bobosha, Demissie (CR14) 2010; 40 Bowtell (CR2) 1999; 21 Lonnroth, Castro, Chakaya, Chauhan, Floyd, Glaziou (CR3) 2010; 375 Demissie, Abebe, Aseffa, Rook, Fletcher, Zumla (CR10) 2004; 172 Abebe, Bjune (CR20) 2009; 157 Cooper (CR21) 2009; 27 CR4 Eldering, Spek, Aberson, Grummels, Derks, de Vos (CR7) 2003; 31 Berry, Graham, McNab, Xu, Bloch, Oni (CR16) 2010; 466 Lund, Hsing, Pham, Rudensky (CR24) 2008; 320 CR25 Jacobsen, Repsilber, Gutschmidt, Neher, Feldmann, Mollenkopf (CR13) 2007; 85 Atkinson, Colburn, DeGruttola, DeMets, Downing, Hoth (CR1) 2001; 69 Kaufmann (CR5) 2010; 33 Shafiani, Tucker-Heard, Kariyone, Takatsu, Urdahl (CR23) 2010; 207 Mistry, Cliff, Clayton, Beyers, Mohamed, Wilson (CR18) 2007; 195 Schouten, McElgunn, Waaijer, Zwijnenburg, Diepvens, Pals (CR6) 2002; 30 E Eldering (BFgene201164_CR7) 2003; 31 BFgene201164_CR4 SJ White (BFgene201164_CR8) 2004; 24 M Abebe (BFgene201164_CR14) 2010; 40 A Demissie (BFgene201164_CR10) 2004; 172 JJ Goeman (BFgene201164_CR26) 2010; 38 R Mistry (BFgene201164_CR18) 2007; 195 M Jacobsen (BFgene201164_CR13) 2007; 85 S Burl (BFgene201164_CR22) 2007; 149 JM Lund (BFgene201164_CR24) 2008; 320 R Tibshirani (BFgene201164_CR15) 1996; 58 MG Bonecini-Almeida (BFgene201164_CR9) 2004; 72 TB Knudsen (BFgene201164_CR11) 2005; 11 S Shafiani (BFgene201164_CR23) 2010; 207 RG Jenner (BFgene201164_CR17) 2005; 3 AJ Atkinson (BFgene201164_CR1) 2001; 69 AM Cooper (BFgene201164_CR21) 2009; 27 C Lienhardt (BFgene201164_CR12) 2002; 32 JP Schouten (BFgene201164_CR6) 2002; 30 BFgene201164_CR25 K Lonnroth (BFgene201164_CR3) 2010; 375 DD Bowtell (BFgene201164_CR2) 1999; 21 SH Kaufmann (BFgene201164_CR5) 2010; 33 J Maertzdorf (BFgene201164_CR19) 2011; 12 MP Berry (BFgene201164_CR16) 2010; 466 N Meinshausen (BFgene201164_CR27) 2008; 95 F Abebe (BFgene201164_CR20) 2009; 157
References_xml	– volume: 157 start-page: 235 year: 2009 end-page: 243 ident: CR20 article-title: The protective role of antibody responses during infection publication-title: Clin Exp Immunol doi: 10.1111/j.1365-2249.2009.03967.x – volume: 30 start-page: e57 year: 2002 ident: CR6 article-title: Relative quantification of 40 nucleic acid sequences by multiplex ligation-dependent probe amplification publication-title: Nucleic Acids Res doi: 10.1093/nar/gnf056 – ident: CR4 – volume: 58 start-page: 267 year: 1996 end-page: 288 ident: CR15 article-title: Regression shrinkage and selection via the lasso publication-title: J Royal Statist Soc B – volume: 3 start-page: 281 year: 2005 end-page: 294 ident: CR17 article-title: Insights into host responses against pathogens from transcriptional profiling publication-title: Nat Rev Microbiol doi: 10.1038/nrmicro1126 – volume: 72 start-page: 2628 year: 2004 end-page: 2634 ident: CR9 article-title: Down-modulation of lung immune responses by interleukin-10 and transforming growth factor beta (TGF-beta) and analysis of TGF-beta receptors I and II in active tuberculosis publication-title: Infect Immun doi: 10.1128/IAI.72.5.2628-2634.2004 – volume: 11 start-page: 730 year: 2005 end-page: 735 ident: CR11 article-title: Predictive value of soluble haemoglobin scavenger receptor CD163 serum levels for survival in verified tuberculosis patients publication-title: Clin Microbiol Infect doi: 10.1111/j.1469-0691.2005.01229.x – volume: 85 start-page: 613 year: 2007 end-page: 621 ident: CR13 article-title: Candidate biomarkers for discrimination between infection and disease caused by Mycobacterium tuberculosis publication-title: J Mol Med doi: 10.1007/s00109-007-0157-6 – volume: 149 start-page: 117 year: 2007 end-page: 122 ident: CR22 article-title: FOXP3 gene expression in a tuberculosis case contact study publication-title: Clin Exp Immunol doi: 10.1111/j.1365-2249.2007.03399.x – volume: 32 start-page: 1605 year: 2002 end-page: 1613 ident: CR12 article-title: Active tuberculosis in Africa is associated with reduced Th1 and increased Th2 activity publication-title: Eur J Immunol doi: 10.1002/1521-4141(200206)32:6<1605::AID-IMMU1605>3.0.CO;2-6 – volume: 320 start-page: 1220 year: 2008 end-page: 1224 ident: CR24 article-title: Coordination of early protective immunity to viral infection by regulatory T cells publication-title: Science doi: 10.1126/science.1155209 – volume: 27 start-page: 393 year: 2009 end-page: 422 ident: CR21 article-title: Cell-mediated immune responses in tuberculosis publication-title: Annu Rev Immunol doi: 10.1146/annurev.immunol.021908.132703 – volume: 38 start-page: 3782 year: 2010 end-page: 3810 ident: CR26 article-title: The sequential rejection principle of familywise error control publication-title: Ann Statist doi: 10.1214/10-AOS829 – volume: 12 start-page: 15 year: 2011 end-page: 22 ident: CR19 article-title: Human gene expression profiles of susceptibility and resistance in tuberculosis publication-title: Genes Immun doi: 10.1038/gene.2010.51 – ident: CR25 – volume: 33 start-page: 567 year: 2010 end-page: 577 ident: CR5 article-title: Future vaccination strategies against tuberculosis: thinking outside the box publication-title: Immunity doi: 10.1016/j.immuni.2010.09.015 – volume: 31 start-page: e153 year: 2003 ident: CR7 article-title: Expression profiling via novel multiplex assay allows rapid assessment of gene regulation in defined signalling pathways publication-title: Nucleic Acids Res doi: 10.1093/nar/gng153 – volume: 24 start-page: 86 year: 2004 end-page: 92 ident: CR8 article-title: Two-color multiplex ligation-dependent probe amplification: detecting genomic rearrangements in hereditary multiple exostoses publication-title: Hum Mutat doi: 10.1002/humu.20054 – volume: 207 start-page: 1409 year: 2010 end-page: 1420 ident: CR23 article-title: Pathogen-specific regulatory T cells delay the arrival of effector T cells in the lung during early tuberculosis publication-title: J Exp Med doi: 10.1084/jem.20091885 – volume: 375 start-page: 1814 year: 2010 end-page: 1829 ident: CR3 article-title: Tuberculosis control and elimination 2010–50: cure, care, and social development publication-title: Lancet doi: 10.1016/S0140-6736(10)60483-7 – volume: 40 start-page: 291 year: 2010 end-page: 301 ident: CR14 article-title: Expression of apoptosis-related genes in an Ethiopian cohort study correlates with tuberculosis clinical status publication-title: Eur J Immunol doi: 10.1002/eji.200939856 – volume: 95 start-page: 265 year: 2008 end-page: 278 ident: CR27 article-title: Hierarchical testing of variable importance publication-title: Biometrika doi: 10.1093/biomet/asn007 – volume: 69 start-page: 89 year: 2001 end-page: 95 ident: CR1 article-title: Biomarkers and surrogate endpoints: preferred definitions and conceptual framework publication-title: Clin Pharmacol Ther doi: 10.1067/mcp.2001.113989 – volume: 195 start-page: 357 year: 2007 end-page: 365 ident: CR18 article-title: Gene-expression patterns in whole blood identify subjects at risk for recurrent tuberculosis publication-title: J Infect Dis doi: 10.1086/510397 – volume: 172 start-page: 6938 year: 2004 end-page: 6943 ident: CR10 article-title: Healthy individuals that control a latent infection with Mycobacterium tuberculosis express high levels of Th1 cytokines and the IL-4 antagonist IL-4delta2 publication-title: J Immunol doi: 10.4049/jimmunol.172.11.6938 – volume: 466 start-page: 973 year: 2010 end-page: 977 ident: CR16 article-title: An interferon-inducible neutrophil-driven blood transcriptional signature in human tuberculosis publication-title: Nature doi: 10.1038/nature09247 – volume: 21 start-page: 25 year: 1999 end-page: 32 ident: CR2 article-title: Options available—from start to finish—for obtaining expression data by microarray publication-title: Nat Genet doi: 10.1038/4455 – volume: 195 start-page: 357 year: 2007 ident: BFgene201164_CR18 publication-title: J Infect Dis doi: 10.1086/510397 – volume: 72 start-page: 2628 year: 2004 ident: BFgene201164_CR9 publication-title: Infect Immun doi: 10.1128/IAI.72.5.2628-2634.2004 – volume: 95 start-page: 265 year: 2008 ident: BFgene201164_CR27 publication-title: Biometrika doi: 10.1093/biomet/asn007 – volume: 157 start-page: 235 year: 2009 ident: BFgene201164_CR20 publication-title: Clin Exp Immunol doi: 10.1111/j.1365-2249.2009.03967.x – volume: 30 start-page: e57 year: 2002 ident: BFgene201164_CR6 publication-title: Nucleic Acids Res doi: 10.1093/nar/gnf056 – ident: BFgene201164_CR25 doi: 10.1002/9780470316856 – volume: 32 start-page: 1605 year: 2002 ident: BFgene201164_CR12 publication-title: Eur J Immunol doi: 10.1002/1521-4141(200206)32:6<1605::AID-IMMU1605>3.0.CO;2-6 – volume: 24 start-page: 86 year: 2004 ident: BFgene201164_CR8 publication-title: Hum Mutat doi: 10.1002/humu.20054 – volume: 375 start-page: 1814 year: 2010 ident: BFgene201164_CR3 publication-title: Lancet doi: 10.1016/S0140-6736(10)60483-7 – volume: 3 start-page: 281 year: 2005 ident: BFgene201164_CR17 publication-title: Nat Rev Microbiol doi: 10.1038/nrmicro1126 – volume: 21 start-page: 25 year: 1999 ident: BFgene201164_CR2 publication-title: Nat Genet doi: 10.1038/4455 – volume: 38 start-page: 3782 year: 2010 ident: BFgene201164_CR26 publication-title: Ann Statist doi: 10.1214/10-AOS829 – volume: 172 start-page: 6938 year: 2004 ident: BFgene201164_CR10 publication-title: J Immunol doi: 10.4049/jimmunol.172.11.6938 – volume: 33 start-page: 567 year: 2010 ident: BFgene201164_CR5 publication-title: Immunity doi: 10.1016/j.immuni.2010.09.015 – volume: 12 start-page: 15 year: 2011 ident: BFgene201164_CR19 publication-title: Genes Immun doi: 10.1038/gene.2010.51 – volume: 149 start-page: 117 year: 2007 ident: BFgene201164_CR22 publication-title: Clin Exp Immunol doi: 10.1111/j.1365-2249.2007.03399.x – volume: 207 start-page: 1409 year: 2010 ident: BFgene201164_CR23 publication-title: J Exp Med doi: 10.1084/jem.20091885 – volume: 31 start-page: e153 year: 2003 ident: BFgene201164_CR7 publication-title: Nucleic Acids Res doi: 10.1093/nar/gng153 – volume: 11 start-page: 730 year: 2005 ident: BFgene201164_CR11 publication-title: Clin Microbiol Infect doi: 10.1111/j.1469-0691.2005.01229.x – volume: 27 start-page: 393 year: 2009 ident: BFgene201164_CR21 publication-title: Annu Rev Immunol doi: 10.1146/annurev.immunol.021908.132703 – volume: 466 start-page: 973 year: 2010 ident: BFgene201164_CR16 publication-title: Nature doi: 10.1038/nature09247 – volume: 40 start-page: 291 year: 2010 ident: BFgene201164_CR14 publication-title: Eur J Immunol doi: 10.1002/eji.200939856 – ident: BFgene201164_CR4 – volume: 85 start-page: 613 year: 2007 ident: BFgene201164_CR13 publication-title: J Mol Med doi: 10.1007/s00109-007-0157-6 – volume: 58 start-page: 267 year: 1996 ident: BFgene201164_CR15 publication-title: J Royal Statist Soc B doi: 10.1111/j.2517-6161.1996.tb02080.x – volume: 320 start-page: 1220 year: 2008 ident: BFgene201164_CR24 publication-title: Science doi: 10.1126/science.1155209 – volume: 69 start-page: 89 year: 2001 ident: BFgene201164_CR1 publication-title: Clin Pharmacol Ther doi: 10.1067/mcp.2001.113989
SSID	ssj0016169
Score	2.3079433
Snippet	Owing to our lack of understanding of the factors that constitute protective immunity during natural infection with Mycobacterium tuberculosis ( Mtb ), there... Owing to our lack of understanding of the factors that constitute protective immunity during natural infection with Mycobacterium tuberculosis (Mtb), there is...
SourceID	proquest gale pubmed crossref springer
SourceType	Aggregation Database Index Database Enrichment Source Publisher
StartPage	71
SubjectTerms	Adaptive immunity Apoptosis Biological markers Biomarkers Biomedical and Life Sciences Biomedicine Cancer Research Cohort analysis Diagnosis Gene Expression Gene Expression Profiling Genes Genetic Markers - genetics Human Genetics Humans Identification Immune response Immune system Immunology Infections Infectious diseases Latent infection Lymphocytes B Lymphocytes T Mycobacterium tuberculosis Nucleic Acid Amplification Techniques - methods original-article Physiological aspects Polymerase chain reaction Real-Time Polymerase Chain Reaction - methods Reproducibility of Results Sensitivity and Specificity Transcriptomics Tuberculosis Tuberculosis - genetics Tuberculosis - immunology Vaccines
Title	Identification of biomarkers for tuberculosis disease using a novel dual-color RT–MLPA assay
URI	https://link.springer.com/article/10.1038/gene.2011.64 https://www.ncbi.nlm.nih.gov/pubmed/21956656 https://www.proquest.com/docview/2641736995 https://www.proquest.com/docview/916875872 https://www.proquest.com/docview/917154446 https://www.proquest.com/docview/968166165
Volume	13
WOSCitedRecordID	wos000299596900009&url=https%3A%2F%2Fcvtisr.summon.serialssolutions.com%2F%23%21%2Fsearch%3Fho%3Df%26include.ft.matches%3Dt%26l%3Dnull%26q%3D
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
journalDatabaseRights	– providerCode: PRVPQU databaseName: Biological Science Database customDbUrl: eissn: 1476-5470 dateEnd: 20171231 omitProxy: false ssIdentifier: ssj0016169 issn: 1466-4879 databaseCode: M7P dateStart: 19990901 isFulltext: true titleUrlDefault: http://search.proquest.com/biologicalscijournals providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Central customDbUrl: eissn: 1476-5470 dateEnd: 20171231 omitProxy: false ssIdentifier: ssj0016169 issn: 1466-4879 databaseCode: BENPR dateStart: 19990901 isFulltext: true titleUrlDefault: https://www.proquest.com/central providerName: ProQuest – providerCode: PRVPQU databaseName: ProQuest Health & Medical Collection customDbUrl: eissn: 1476-5470 dateEnd: 20171231 omitProxy: false ssIdentifier: ssj0016169 issn: 1466-4879 databaseCode: 7X7 dateStart: 19990901 isFulltext: true titleUrlDefault: https://search.proquest.com/healthcomplete providerName: ProQuest – providerCode: PRVPQU databaseName: Public Health Database customDbUrl: eissn: 1476-5470 dateEnd: 20171231 omitProxy: false ssIdentifier: ssj0016169 issn: 1466-4879 databaseCode: 8C1 dateStart: 19990901 isFulltext: true titleUrlDefault: https://search.proquest.com/publichealth providerName: ProQuest
link	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1La9wwEB6apIVe-n64SRdR-jgUUUuyJflUtiGhhXZZtinsoWAsrRwCi53a60D-fTW2dttN21x60UUfltBIo8-aF8BLmVphithQ7hYZTdzCUpNoQy1XqmTSSatMX2xCTSZ6Ps-mwTenDW6Va53YK-pFbfGN_J2nMZ5aa8Xfn_-gWDQKjauhgsYO7GGSBNF77k03RgTJ-op2XhdI6nl5FvzeY-H_-bwiGfJ3ymTrRrqql3-7mK5YSvsL6Pjuf079HtwJzJOMh61yH2646gHcGmpRXj6E70PIbhne8EhdEgzNR--dpiWe2pJVZ1xju2XdnrUkGHYI-s2fkoJU9YVbEgzsopgHuyGzE_rl83RMPDkvLh_Bt-Ojk8OPNJReoDZlckVZVjBpMqNdFjvtRBpbyWNXulKZ2All8Z86SfhCpNoYp5k1yhas0NZxXqZSPIbdqq7cUyAJ49YTBRkLh0bUrEgNYybT1isbLq2I4O16-XMb8pJjeYxl3tvHhc5RWDkKK5dJBK826PMhH8c_cC9QkjmmuKjQh-a06No2__R1lo-50lJ4YqcjeBNAZe2HtEUISfATx6xYW8iDLaQ_g3a7ey35POiANvdUkykhsyyNYP_P7s2uiIBsevG76PVWubpDiMJkSYm8BiLR8MukH-PJsFE3y8IxFNTT9Qher3fur9H_tmbPrp3mPtz2OD48Px3A7qrp3HO4aS9WZ20zgh01V32rfasP2Qj2PhxNprNRfyZ_AtWxOQY
linkProvider	ProQuest
linkToHtml	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1R3bbtMw1BoDBC_cL2EDLMTgAUXEdmI7DwhVwLRqXVWNIe1hkoldZ5pUJaNph_pR_CPn5FLogL3tgWef2I59rj43Ql7KxAmbRTbkfpyGsR-70Mbaho4rlTPppVO2bjahhkN9eJiO1siPLhcGwyo7nlgz6nHp8I38LagxoFprxd-ffguxaRQ6V7sOGg1W7PrFd7DYqnf9j3C9W5xvfzr4sBO2TQVClzA5C1maMWlTq30aee1FEjnJI5_7XNnIC-XQWoxjPhaJttZr5qxyGcu085zniRQw7xVyFbQIrutIwdHSaSFZ3UEPeI8MwQ5I2zj7SICNCYyrqRcq4xUJeF4O_CYIz3lma4G3ffs_O6o75FarWdNeQwp3yZov7pHrTa_NxX1y1KQk5-0bJS1ziqUHMDppWlFQ3elsbv3UzSdldVLR1nFFMS_gmGa0KM_8hGLiWoh1vqd0_yDcG4x6FIyPbPGAfLmUX3tI1ouy8I8JBURwoAjJSHh0EqdZYhmzqXbATLl0IiBvuus2rq27ju0_Jqb2_wttEDkMIoeRcUC2ltCnTb2Rf8C9QMwxWMKjwBih42xeVab_ed_0uNJSgOKqA_K6BcpLWNJlbcoFbByrfq1Abq5AAo9xq8MdppmWx1UGVGmmhEzTJCAbfw4vsTAgdDmK82JUX-HLOYIoLAYVywtAJDq2mYQ1HjWEsTwWjqmuYI4E5FVHKb9W_9uZPblwm8_JjZ2DvYEZ9Ie7G-QmfMObp7ZNsj6bzv1Tcs2dzU6q6bOa6in5etm08xNVqJKT
linkToPdf	http://cvtisr.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMw1R3JbtQw1CplERf2JbSAhSgckNXYSWzngNCoZcSoZTQqReoBycQep6o0Sspkpmg-jb_jvSwDU6C3Hjj7xXbst_pthLyUiYtsFlom_DhlsR87ZmNtmRNK5Vx66ZStm02o4VAfHaWjNfKjy4XBsMqOJ9aMelw6fCPfBjUGVGutxHbeRkWMdvvvTr8xbCCFjtaum0aDIXt-8R2st-rtYBeuekuI_vvDnQ-sbTDAXMLljPE049KmVvs09NpHSeikCH3uc2VDHymHlmMci3GUaGu95s4ql_FMOy9EnsgI5r1CrqoIkBiT1HeW0SWgR9Xd9IAPSQY2QdrG3IcR2JvAxJraoTJekYbnZcJvQvGcl7YWfv3b__Gx3SG3Wo2b9hoSuUvWfHGPXG96cC7uky9NqnLevl3SMqdYkgCjlqYVBZWezubWT918UlYnFW0dWhTzBY5pRovyzE8oJrQxrP89pQeH7OP-qEfBKMkWD8jnS_m1h2S9KAv_mNCYCwcKkgwjj87jNEss5zbVDpiskC4KyJvu6o1r67FjW5CJqeMCIm0QUQwiipFxQLaW0KdNHZJ_wL1ALDJY2qPA6z7O5lVlBp8OTE8oLSNQaHVAXrdAeQlLuqxNxYCNYzWwFcjNFUjgPW51uMM60_K-yoCKzYEk0jQJyMafw0uMDAhdjuK8GO1X-HKOIAqLRMXyAhCJDm8uYY1HDZEsj0VgCiyYKQF51VHNr9X_dmZPLtzmc3IDSMbsD4Z7G-QmfCKaF7hNsj6bzv1Tcs2dzU6q6bOaAVDy9bJJ5ydxopsJ
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Identification+of+biomarkers+for+tuberculosis+disease+using+a+novel+dual-color+RT-MLPA+assay&rft.jtitle=Genes+and+immunity&rft.au=Joosten%2C+S.A&rft.au=Goeman%2C+J.J&rft.au=Sutherland%2C+J.S&rft.au=Opmeer%2C+L&rft.date=2012-01-01&rft.pub=Nature+Publishing+Group&rft.issn=1466-4879&rft.volume=13&rft.issue=1&rft.spage=71&rft_id=info:doi/10.1038%2Fgene.2011.64&rft.externalDocID=A278631258
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1466-4879&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1466-4879&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1466-4879&client=summon