Quantification of Apoptosis and Necrosis by Flow Cytometry

Apoptosis and necrosis are two important mechanisms of cell death. Several methods have recently been described for quantifying apoptotic cells by flow cytometry. We report a novel method for the quantification and separation of viable normal and apoptotic cells. We have applied this method both to...

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Bibliographic Details
Published in:	Acta oncologica Vol. 32; no. 4; pp. 417 - 424
Main Authors:	Ormerod, Michael G., Sun, Xiao-Ming, Brown, David, Snowden, Roger T., Cohen, Gerald M.
Format:	Journal Article Conference Proceeding
Language:	English
Published:	Basingstoke Informa UK Ltd 1993 Taylor & Francis
Subjects:	Ageing, cell death Animals Apoptosis - physiology Benzimidazoles - pharmacokinetics Biological and medical sciences Cell Death - physiology Cell Membrane - metabolism Cell physiology DNA - analysis Flow Cytometry Fluorescent Dyes - pharmacokinetics Fundamental and applied biological sciences. Psychology Male Mice Molecular and cellular biology Necrosis - pathology Propidium - pharmacokinetics Rats Rats, Inbred F344 Thymus Gland - chemistry Thymus Gland - cytology Trypan Blue - pharmacokinetics Flow cytometry Vertebrata Mammalia Rat Mouse Cell death Thymocyte Animal Rodentia Hematopoietic cell Quantization Necrosis
ISSN:	0284-186X, 1651-226X
Online Access:	Get full text
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