Viromes of Tabanids from Russia

Advances in sequencing technologies and bioinformatics have greatly enhanced our knowledge of virus biodiversity. Currently, the viromes of hematophagous invertebrates, such as mosquitoes and ixodid ticks, are being actively studied. Tabanidae (Diptera) are a widespread family, with members mostly k...

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Bibliographic Details
Published in:Viruses Vol. 15; no. 12; p. 2368
Main Authors: Litov, Alexander G., Belova, Oxana A., Kholodilov, Ivan S., Kalyanova, Anna S., Gadzhikurbanov, Magomed N., Rogova, Anastasia A., Gmyl, Larissa V., Karganova, Galina G.
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 30.11.2023
Subjects:
Algorithms
Biodiversity
Bioinformatics
Cattle
Cell culture
Cell lines
Chrysops
Culicidae
Diptera
Discovery and exploration
Distribution
Experiments
Flaviviridae
genome
Genomes
Haematopota
Health aspects
Homogenization
Hybomitra
Insect viruses
Invertebrates
Kidneys
Life sciences
Milk production
Narnaviridae
Next-generation sequencing
Pathogens
Permutotetraviridae
Phylogenetics
Russia
swine
Tabanidae
Tabanus
ticks
Totiviridae
viral load
Virus research
Viruses
Xinmoviridae
Russia
South Korea
United Kingdom > UK
United States > US
negevirus
Rhabdoviridae
Narnaviridae
Solemoviridae
Xinmoviridae
Totiviridae
Flaviviridae
Permutotetraviridae
Picornavirales
Tabanidae
ISSN:1999-4915, 1999-4915
Online Access:Get full text
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Summary:Advances in sequencing technologies and bioinformatics have greatly enhanced our knowledge of virus biodiversity. Currently, the viromes of hematophagous invertebrates, such as mosquitoes and ixodid ticks, are being actively studied. Tabanidae (Diptera) are a widespread family, with members mostly known for their persistent hematophagous behavior. They transmit viral, bacterial, and other pathogens, both biologically and mechanically. However, tabanid viromes remain severely understudied. In this study, we used high-throughput sequencing to describe the viromes of several species in the Hybomitra, Tabanus, Chrysops, and Haematopota genera, which were collected in two distant parts of Russia: the Primorye Territory and Ryazan Region. We assembled fourteen full coding genomes of novel viruses, four partial coding genomes, as well as several fragmented viral sequences, which presumably belong to another twelve new viruses. All the discovered viruses were tested for their ability to replicate in mammalian porcine embryo kidney (PEK), tick HAE/CTVM8, and mosquito C6/36 cell lines. In total, 16 viruses were detected in at least one cell culture after three passages (for PEK and C6/36) or 3 weeks of persistence in HAE/CTVM8. However, in the majority of cases, qPCR showed a decline in virus load over time.
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ISSN:1999-4915
1999-4915
DOI:10.3390/v15122368