Vitamin D and Vitamin K Concentrations in Human Brain Tissue Are Influenced by Freezer Storage Time: The Memory and Aging Project

Vitamins D and K, which are present in human brain, may have a role in neurodegenerative disease. Given the interest in measuring nutrient concentrations in archived brain samples, it is important to evaluate whether freezer storage time affects these concentrations. Therefore, we evaluated differen...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of nutrition Vol. 151; no. 1; p. 104
Main Authors: Fu, Xueyan, Shea, M Kyla, Dolnikowski, Gregory G, Patterson, William B, Dawson-Hughes, Bess, Holland, Thomas M, Schneider, Julie A, Booth, Sarah L
Format: Journal Article
Language:English
Published: United States 01.01.2021
Subjects:
Adolescent
Adult
Aged
Aged, 80 and over
Brain Chemistry
Cross-Sectional Studies
Female
Humans
Male
Middle Aged
Retrospective Studies
Specimen Handling
Time Factors
Vitamin D - chemistry
Vitamin K - chemistry
Young Adult
storage
brain
vitamin K
vitamin D
stability
Alzheimer disease and related dementias
ISSN:1541-6100, 1541-6100
Online Access:Get more information
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Vitamins D and K, which are present in human brain, may have a role in neurodegenerative disease. Given the interest in measuring nutrient concentrations in archived brain samples, it is important to evaluate whether freezer storage time affects these concentrations. Therefore, we evaluated differences in vitamin D and vitamin K concentrations in human brain samples stored for various lengths of time. Postmortem brain samples were obtained from 499 participants in the Rush Memory and Aging Project (mean age 92 y, 72% female). Concentrations of vitamins D and K and their metabolites were measured in 4 regions (midtemporal cortex, midfrontal cortex, cerebellum, anterior watershed white matter) using LC-MS/MS and HPLC, respectively. The predominant forms were 25-hydroxycholecalciferol [25(OH)D3] and menaquinone-4 (MK4). ANOVA was used to determine if concentrations differed according to storage time. The geometric mean of the mean 25(OH)D3 concentration (across 4 regions) in brains stored for 1.1 to 6.0 y did not differ from that in brains stored ≤1.0 y (all P ≥ 0.37), whereas 25(OH)D3 in brains stored >6.0 y was 31-40% lower (P ≤ 0.003). MK4 had similar results, with the geometric mean MK4 concentration in the brains stored ≥9.0 y being 48-52% lower than those in brains stored ≤1.0 y (P ≤ 0.012). The 25(OH)D3 and MK4 concentrations were positively correlated across all 4 regions (all Spearman ρ ≥ 0.79, P < 0.001). 25(OH)D3 and MK4 appear to be stable in brain tissue from older adults stored at -80°C for up to 6 and 9 y, respectively, but not longer. Freezer storage time should be considered in the design and interpretation of studies using archived brain tissue.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1541-6100
1541-6100
DOI:10.1093/jn/nxaa336