Comprehensive evaluation of methods for small extracellular vesicles separation from human plasma, urine and cell culture medium

One of the challenges that restricts the evolving extracellular vesicle (EV) research field is the lack of a consensus method for EV separation. This may also explain the diversity of the experimental results, as co‐separated soluble proteins and lipoproteins may impede the interpretation of experim...

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Bibliographic Details
Published in:	Journal of extracellular vesicles Vol. 10; no. 2; pp. e12044 - n/a
Main Authors:	Dong, Liang, Zieren, Richard C., Horie, Kengo, Kim, Chi‐Ju, Mallick, Emily, Jing, Yuezhou, Feng, Mingxiao, Kuczler, Morgan D., Green, Jordan, Amend, Sarah R., Witwer, Kenneth W., de Reijke, Theo M., Cho, Yoon‐Kyoung, Pienta, Kenneth J., Xue, Wei
Format:	Journal Article
Language:	English
Published:	United States John Wiley & Sons, Inc 01.12.2020 John Wiley and Sons Inc
Subjects:	Biofiltration Cell culture Chromatography, Gel - methods Contaminants Culture media Culture Media - chemistry EV yield Extracellular vesicles Extracellular Vesicles - metabolism Humans Invoices Lipoproteins Male methods comparison Microfluidics Phenotyping Plasma Plasma - chemistry Plasma - metabolism Prostatic Neoplasms - metabolism Reagents sample purity single particle phenotyping small extracellular vesicle separation Tumor Cells, Cultured Ultracentrifugation Ultracentrifugation - methods Ultrafiltration Urine Urine - chemistry United States > US EV yield sample purity methods comparison small extracellular vesicle separation single particle phenotyping
ISSN:	2001-3078, 2001-3078
Online Access:	Get full text
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