Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging

Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employe...

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Vydáno v:Nature methods Ročník 20; číslo 8; s. 1174 - 1178
Hlavní autoři: Quardokus, Ellen M., Saunders, Diane C., McDonough, Elizabeth, Hickey, John W., Werlein, Christopher, Surrette, Christine, Rajbhandari, Presha, Casals, Anna Martinez, Tian, Hua, Lowery, Lisa, Neumann, Elizabeth K., Björklund, Frida, Neelakantan, Taruna V., Croteau, Josh, Wiblin, Anne E., Fisher, Jeremy, Livengood, April J., Dowell, Karen G., Silverstein, Jonathan C., Spraggins, Jeffrey M., Pryhuber, Gloria S., Deutsch, Gail, Ginty, Fiona, Nolan, Garry P., Melov, Simon, Jonigk, Danny, Caldwell, Michael A., Vlachos, Ioannis S., Muller, Werner, Gehlenborg, Nils, Stockwell, Brent R., Lundberg, Emma, Snyder, Michael P., Germain, Ronald N., Camarillo, Jeannie M., Kelleher, Neil L., Börner, Katy, Radtke, Andrea J.
Médium: Journal Article
Jazyk:angličtina
Vydáno: New York Nature Publishing Group US 01.08.2023
Nature Publishing Group
Témata:
631/1647/245/2226
631/61
631/80/2373
706/648
Antibodies
Bioinformatics
Biological Microscopy
Biological Techniques
Biomarkers
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Brief Communication
Cloning
Communication
Community Resources
Construction
Costs
Data collection
Diagnostic Imaging
Humans
Imaging
Life Sciences
Lymphatic system
Lymphocytes
Mapping
Medical research
Multiplexing
Panels
Proteins
Proteomics
Reproducibility of Results
Subject specialists
ISSN:1548-7091, 1548-7105, 1548-7105
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Popis
Shrnutí:Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods. Organ mapping antibody panels are community-validated resources that save time and money, increase reproducibility, accelerate discovery and support the construction of a Human Reference Atlas. Organ Mapping Antibody Panels are a community-led initiative to create standardized antibody panels for multiplexed spatial imaging.
Bibliografie:ObjectType-Article-1
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ISSN:1548-7091
1548-7105
1548-7105
DOI:10.1038/s41592-023-01846-7