Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging
Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employe...
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| Vydáno v: | Nature methods Ročník 20; číslo 8; s. 1174 - 1178 |
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| Hlavní autoři: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
| Médium: | Journal Article |
| Jazyk: | angličtina |
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New York
Nature Publishing Group US
01.08.2023
Nature Publishing Group |
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| ISSN: | 1548-7091, 1548-7105, 1548-7105 |
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| Abstract | Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods. Organ mapping antibody panels are community-validated resources that save time and money, increase reproducibility, accelerate discovery and support the construction of a Human Reference Atlas.
Organ Mapping Antibody Panels are a community-led initiative to create standardized antibody panels for multiplexed spatial imaging. |
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| AbstractList | Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods. Organ mapping antibody panels are community-validated resources that save time and money, increase reproducibility, accelerate discovery and support the construction of a Human Reference Atlas. Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods. Organ mapping antibody panels are community-validated resources that save time and money, increase reproducibility, accelerate discovery and support the construction of a Human Reference Atlas.Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods. Organ mapping antibody panels are community-validated resources that save time and money, increase reproducibility, accelerate discovery and support the construction of a Human Reference Atlas. Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods. Organ mapping antibody panels are community-validated resources that save time and money, increase reproducibility, accelerate discovery and support the construction of a Human Reference Atlas. Organ Mapping Antibody Panels are a community-led initiative to create standardized antibody panels for multiplexed spatial imaging. Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods. Organ mapping antibody panels are community-validated resources that save time and money, increase reproducibility, accelerate discovery and support the construction of a Human Reference Atlas. Organ Mapping Antibody Panels are a community-led initiative to create standardized antibody panels for multiplexed spatial imaging. Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods. Organ mapping antibody panels are community-validated resources that save time and money, increase reproducibility, accelerate discovery and support the construction of a Human Reference Atlas. |
| Author | Saunders, Diane C. Fisher, Jeremy Camarillo, Jeannie M. Lundberg, Emma Lowery, Lisa Börner, Katy Dowell, Karen G. Snyder, Michael P. Croteau, Josh Jonigk, Danny Caldwell, Michael A. Ginty, Fiona Björklund, Frida Kelleher, Neil L. Casals, Anna Martinez Rajbhandari, Presha Pryhuber, Gloria S. Vlachos, Ioannis S. Werlein, Christopher McDonough, Elizabeth Germain, Ronald N. Tian, Hua Nolan, Garry P. Surrette, Christine Stockwell, Brent R. Melov, Simon Deutsch, Gail Gehlenborg, Nils Hickey, John W. Muller, Werner Radtke, Andrea J. Silverstein, Jonathan C. Neumann, Elizabeth K. Quardokus, Ellen M. Livengood, April J. Spraggins, Jeffrey M. Wiblin, Anne E. Neelakantan, Taruna V. |
| Author_xml | – sequence: 1 givenname: Ellen M. orcidid: 0000-0001-7655-4833 surname: Quardokus fullname: Quardokus, Ellen M. organization: Department of Intelligent Systems Engineering, Indiana University – sequence: 2 givenname: Diane C. orcidid: 0000-0002-8849-6746 surname: Saunders fullname: Saunders, Diane C. organization: Department of Medicine, Vanderbilt University Medical Center, Vanderbilt Diabetes Center, Vanderbilt University School of Medicine – sequence: 3 givenname: Elizabeth orcidid: 0000-0001-7524-8260 surname: McDonough fullname: McDonough, Elizabeth organization: GE Research – sequence: 4 givenname: John W. orcidid: 0000-0001-9961-7673 surname: Hickey fullname: Hickey, John W. organization: Department of Pathology, Stanford University School of Medicine – sequence: 5 givenname: Christopher orcidid: 0000-0002-7694-4257 surname: Werlein fullname: Werlein, Christopher organization: Institute of Pathology, Hannover Medical School – sequence: 6 givenname: Christine surname: Surrette fullname: Surrette, Christine organization: GE Research – sequence: 7 givenname: Presha surname: Rajbhandari fullname: Rajbhandari, Presha organization: Department of Biological Sciences, Columbia University – sequence: 8 givenname: Anna Martinez surname: Casals fullname: Casals, Anna Martinez organization: Department of Pathology, Stanford University School of Medicine, Science for Life Laboratory, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, Department of Bioengineering, Stanford University – sequence: 9 givenname: Hua orcidid: 0000-0002-3598-0219 surname: Tian fullname: Tian, Hua organization: Department of Chemistry, Pennsylvania State University – sequence: 10 givenname: Lisa surname: Lowery fullname: Lowery, Lisa organization: GE Research – sequence: 11 givenname: Elizabeth K. surname: Neumann fullname: Neumann, Elizabeth K. organization: Department of Biochemistry, Vanderbilt University, Mass Spectrometry Research Center, Vanderbilt University, Department of Chemistry, University of California Davis – sequence: 12 givenname: Frida surname: Björklund fullname: Björklund, Frida organization: Science for Life Laboratory, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology – sequence: 13 givenname: Taruna V. surname: Neelakantan fullname: Neelakantan, Taruna V. organization: Department of Chemistry, Columbia University – sequence: 14 givenname: Josh orcidid: 0000-0002-8142-0482 surname: Croteau fullname: Croteau, Josh organization: Department of Business Development, BioLegend Inc – sequence: 15 givenname: Anne E. orcidid: 0000-0002-6087-5701 surname: Wiblin fullname: Wiblin, Anne E. organization: Department of Research and Development, Abcam PLC, Discovery Drive, Cambridge Biomedical Campus – sequence: 16 givenname: Jeremy orcidid: 0000-0002-7296-586X surname: Fisher fullname: Fisher, Jeremy organization: Department of Research and Development, Cell Signaling Technology, Inc – sequence: 17 givenname: April J. surname: Livengood fullname: Livengood, April J. organization: Department of Protein and Cell Analysis, Thermo Fisher Scientific – sequence: 18 givenname: Karen G. surname: Dowell fullname: Dowell, Karen G. organization: Akoya Biosciences – sequence: 19 givenname: Jonathan C. orcidid: 0000-0002-9252-6039 surname: Silverstein fullname: Silverstein, Jonathan C. organization: Department of Biomedical Informatics, University of Pittsburgh School of Medicine – sequence: 20 givenname: Jeffrey M. orcidid: 0000-0001-9198-5498 surname: Spraggins fullname: Spraggins, Jeffrey M. organization: Department of Biochemistry, Vanderbilt University, Mass Spectrometry Research Center, Vanderbilt University, Department of Chemistry, Vanderbilt University, Department of Cell and Developmental Biology, Vanderbilt University – sequence: 21 givenname: Gloria S. orcidid: 0000-0002-9185-3994 surname: Pryhuber fullname: Pryhuber, Gloria S. organization: Department of Pediatrics, University of Rochester Medical Center – sequence: 22 givenname: Gail surname: Deutsch fullname: Deutsch, Gail organization: Department of Pathology, University of Washington Medical Center – sequence: 23 givenname: Fiona surname: Ginty fullname: Ginty, Fiona organization: GE Research – sequence: 24 givenname: Garry P. surname: Nolan fullname: Nolan, Garry P. organization: Department of Pathology, Stanford University School of Medicine – sequence: 25 givenname: Simon orcidid: 0000-0001-8554-2834 surname: Melov fullname: Melov, Simon organization: Buck Institute for Research on Aging – sequence: 26 givenname: Danny orcidid: 0000-0002-5251-2281 surname: Jonigk fullname: Jonigk, Danny organization: Institute of Pathology, Hannover Medical School, German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Institute of Pathology, RWTH University of Aachen – sequence: 27 givenname: Michael A. surname: Caldwell fullname: Caldwell, Michael A. organization: Departments of Chemistry, Molecular Biosciences and the Proteomics Center of Excellence, Northwestern University – sequence: 28 givenname: Ioannis S. orcidid: 0000-0002-8849-808X surname: Vlachos fullname: Vlachos, Ioannis S. organization: Spatial Technologies Unit, Harvard Medical School Initiative for RNA Medicine, Beth Israel Deaconess Medical Center, Department of Pathology, Beth Israel Deaconess Medical Center, Broad Institute of MIT and Harvard – sequence: 29 givenname: Werner orcidid: 0000-0002-1297-9725 surname: Muller fullname: Muller, Werner organization: Miltenyi Biotec B.V. and Co. KG, Division of Infection, Immunity and Respiratory Medicine, University of Manchester – sequence: 30 givenname: Nils orcidid: 0000-0003-0327-8297 surname: Gehlenborg fullname: Gehlenborg, Nils organization: Department of Biomedical Informatics, Harvard Medical School – sequence: 31 givenname: Brent R. orcidid: 0000-0002-3532-3868 surname: Stockwell fullname: Stockwell, Brent R. organization: Department of Biological Sciences, Columbia University, Department of Chemistry, Columbia University – sequence: 32 givenname: Emma orcidid: 0000-0001-7034-0850 surname: Lundberg fullname: Lundberg, Emma organization: Department of Pathology, Stanford University School of Medicine, Science for Life Laboratory, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, Department of Bioengineering, Stanford University – sequence: 33 givenname: Michael P. orcidid: 0000-0003-0784-7987 surname: Snyder fullname: Snyder, Michael P. organization: Department of Genetics, Stanford University School of Medicine – sequence: 34 givenname: Ronald N. orcidid: 0000-0003-1495-9143 surname: Germain fullname: Germain, Ronald N. organization: Laboratory of Immune System Biology, Lymphocyte Biology Section and Center for Advanced Tissue Imaging, NIAID, NIH – sequence: 35 givenname: Jeannie M. surname: Camarillo fullname: Camarillo, Jeannie M. organization: Departments of Chemistry, Molecular Biosciences and the Proteomics Center of Excellence, Northwestern University, Inotiv – sequence: 36 givenname: Neil L. orcidid: 0000-0002-8815-3372 surname: Kelleher fullname: Kelleher, Neil L. organization: Departments of Chemistry, Molecular Biosciences and the Proteomics Center of Excellence, Northwestern University – sequence: 37 givenname: Katy orcidid: 0000-0002-3321-6137 surname: Börner fullname: Börner, Katy organization: Department of Intelligent Systems Engineering, Indiana University – sequence: 38 givenname: Andrea J. orcidid: 0000-0003-4379-8967 surname: Radtke fullname: Radtke, Andrea J. email: andrea.radtke@nih.gov organization: Laboratory of Immune System Biology, Lymphocyte Biology Section and Center for Advanced Tissue Imaging, NIAID, NIH |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/37468619$$D View this record in MEDLINE/PubMed https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-338534$$DView record from Swedish Publication Index (Kungliga Tekniska Högskolan) |
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| ContentType | Journal Article |
| Copyright | The Author(s), under exclusive licence to Springer Nature America, Inc. 2023 2023. The Author(s), under exclusive licence to Springer Nature America, Inc. The Author(s), under exclusive licence to Springer Nature America, Inc. 2023. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. |
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| DOI | 10.1038/s41592-023-01846-7 |
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