An integrated genomic and transcriptomic survey of mucormycosis-causing fungi

Mucormycosis is a life-threatening infection caused by Mucorales fungi. Here we sequence 30 fungal genomes, and perform transcriptomics with three representative Rhizopus and Mucor strains and with human airway epithelial cells during fungal invasion, to reveal key host and fungal determinants contr...

Celý popis

Uloženo v:

Podrobná bibliografie
Vydáno v:	Nature communications Ročník 7; číslo 1; s. 12218 - 11
Hlavní autoři:	Chibucos, Marcus C., Soliman, Sameh, Gebremariam, Teclegiorgis, Lee, Hongkyu, Daugherty, Sean, Orvis, Joshua, Shetty, Amol C., Crabtree, Jonathan, Hazen, Tracy H., Etienne, Kizee A., Kumari, Priti, O’Connor, Timothy D., Rasko, David A., Filler, Scott G., Fraser, Claire M., Lockhart, Shawn R., Skory, Christopher D., Ibrahim, Ashraf S., Bruno, Vincent M.
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	London Nature Publishing Group UK 22.07.2016 Nature Publishing Group Nature Portfolio
Témata:	13/106 38/23 38/39 38/91 631/208/325/2484 631/326/193/2540 692/699/255/1672 96/95 A549 Cells Amidohydrolases - metabolism Amino Acid Sequence Animals Annotations Base Sequence Comparative analysis Debridement Disease Economic importance Fungal Proteins - chemistry Fungi Genes, Fungal Genome, Fungal Genomes Humanities and Social Sciences Humans Infections Male Medicine Mice, Inbred ICR Molecular Sequence Annotation Mortality Mucorales - enzymology Mucorales - genetics Mucorales - isolation & purification Mucormycosis - microbiology multidisciplinary Neutropenia Pathogenesis Phylogenetics Phylogeny Polymorphism, Single Nucleotide - genetics Rhizopus - genetics Science Science (multidisciplinary) Sequence Analysis, RNA Species Specificity Transcriptome - genetics Los Angeles California California United States > US Maryland Baltimore Maryland
ISSN:	2041-1723, 2041-1723
On-line přístup:	Získat plný text
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!

Popis
Shrnutí:	Mucormycosis is a life-threatening infection caused by Mucorales fungi. Here we sequence 30 fungal genomes, and perform transcriptomics with three representative Rhizopus and Mucor strains and with human airway epithelial cells during fungal invasion, to reveal key host and fungal determinants contributing to pathogenesis. Analysis of the host transcriptional response to Mucorales reveals platelet-derived growth factor receptor B (PDGFRB) signaling as part of a core response to divergent pathogenic fungi; inhibition of PDGFRB reduces Mucorales-induced damage to host cells. The unique presence of CotH invasins in all invasive Mucorales, and the correlation between CotH gene copy number and clinical prevalence, are consistent with an important role for these proteins in mucormycosis pathogenesis. Our work provides insight into the evolution of this medically and economically important group of fungi, and identifies several molecular pathways that might be exploited as potential therapeutic targets. Fungi of the order Mucorales can cause life-threatening infections. Here, Chibucos et al . present genomic and transcriptomic analyses of a diverse set of Mucorales fungi, shedding light on their evolution and identifying potential therapeutic targets in the pathogens and the host.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Present address: Department of Medicinal Chemistry, College of Pharmacy, University of Sharjah, P.O. Box 27272, Sharjah, UAE.
ISSN:	2041-1723 2041-1723
DOI:	10.1038/ncomms12218