Gold-FISH: A new approach for the in situ detection of single microbial cells combining fluorescence and scanning electron microscopy

A novel fluorescence in situ hybridisation (FISH) method is presented that allows the combination of epifluorescence and scanning electron microscopy (SEM) to identify single microbial cells. First, the rRNA of whole cells is hybridised with horseradish peroxidase-labelled oligonucleotide probes and...

Celý popis

Uloženo v:

Podrobná bibliografie
Vydáno v:	Systematic and applied microbiology Ročník 35; číslo 8; s. 518 - 525
Hlavní autoři:	Schmidt, Hannes, Eickhorst, Thilo, Mußmann, Marc
Médium:	Journal Article Konferenční příspěvek
Jazyk:	angličtina
Vydáno:	München Elsevier GmbH 01.12.2012 Elsevier
Témata:	Bacteriological methods and techniques used in bacteriology Bacteriology binding sites Biological and medical sciences CARD–FISH energy-dispersive X-ray analysis Environmental Microbiology Epifluorescence microscopy fluorescence fluorescence in situ hybridization fluorescence microscopy fluorescent dyes Fluorescent Dyes - metabolism Fundamental and applied biological sciences. Psychology Geologic Sediments - microbiology gold Gold - metabolism Gold-FISH goldfish horseradish In situ hybridisation In Situ Hybridization, Fluorescence - methods light microscopy marine sediments Microbiology microorganisms Microscopy, Electron, Scanning - methods Nanogold Nanoparticles oligonucleotide probes physicochemical properties Plant Roots - microbiology ribosomal RNA rice roots Scanning electron microscopy Staining and Labeling - methods streptavidin Gold-FISH CARD–FISH Scanning electron microscopy Epifluorescence microscopy In situ hybridisation Nanogold Method CARD-FISH Vertebrata Pisces Fluorescence in situ hybridization Isolated cell Detection Fluorescence microscopy
ISSN:	0723-2020, 1618-0984, 1618-0984
On-line přístup:	Získat plný text
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!

Buďte první, kdo okomentuje tento záznam!