Flat clathrin lattices are dynamic actin-controlled hubs for clathrin-mediated endocytosis and signalling of specific receptors

Clathrin lattices at the plasma membrane coat both invaginated and flat regions forming clathrin-coated pits and clathrin plaques, respectively. The function and regulation of clathrin-coated pits in endocytosis are well understood but clathrin plaques remain enigmatic nanodomains. Here we use super...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Nature communications Jg. 8; H. 1; S. 16068 - 14
Hauptverfasser: Leyton-Puig, Daniela, Isogai, Tadamoto, Argenzio, Elisabetta, van den Broek, Bram, Klarenbeek, Jeffrey, Janssen, Hans, Jalink, Kees, Innocenti, Metello
Format: Journal Article
Sprache:Englisch
Veröffentlicht: London Nature Publishing Group UK 13.07.2017
Nature Publishing Group
Nature Portfolio
Schlagworte:
13
13/106
13/89
13/95
14
14/19
14/28
14/63
631/80/128/1276
631/80/313/1461
631/80/86/2368
96
96/10
96/34
96/35
Actin
Actin Cytoskeleton - metabolism
Actin-Related Protein 2-3 Complex - metabolism
AKT protein
Cell adhesion
Cell adhesion & migration
Cell migration
Clathrin
Clathrin - physiology
Clathrin-Coated Vesicles - metabolism
Coated pits
Coating
Control
Dismantling
Endocytosis
Genetics
HeLa Cells
Hubs
Humanities and Social Sciences
Humans
Lattices
Machinery and equipment
multidisciplinary
Pits
Plaques
Polymerization
Receptors
Receptors, Lysophosphatidic Acid - metabolism
Science
Science (multidisciplinary)
Signaling
Wiskott-Aldrich Syndrome Protein, Neuronal - metabolism
ISSN:2041-1723, 2041-1723
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Clathrin lattices at the plasma membrane coat both invaginated and flat regions forming clathrin-coated pits and clathrin plaques, respectively. The function and regulation of clathrin-coated pits in endocytosis are well understood but clathrin plaques remain enigmatic nanodomains. Here we use super-resolution microscopy, molecular genetics and cell biology to show that clathrin plaques contain the machinery for clathrin-mediated endocytosis and cell adhesion, and associate with both clathrin-coated pits and filamentous actin. We also find that actin polymerization promoted by N-WASP through the Arp2/3 complex is crucial for the regulation of plaques but not pits. Clathrin plaques oppose cell migration and undergo actin- and N-WASP-dependent disassembly upon activation of LPA receptor 1, but not EGF receptor. Most importantly, plaque disassembly correlates with the endocytosis of LPA receptor 1 and down-modulation of AKT activity. Thus, clathrin plaques serve as dynamic actin-controlled hubs for clathrin-mediated endocytosis and signalling that exhibit receptor specificity. Clathrin lattices coat flat membrane regions, called plaques, whose regulation and function are poorly understood. Here the authors find that plaques are regulated by actin dynamics and contain both the endocytic and the cell adhesion machineries, and are involved in endocytosis of specific cargos, and cell migration.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
These authors contributed equally to this work.
Present address: Department of Cell Biology; Lyda Hill Department of Bioinformatics, University of Texas Southwestern Medical Center, 6000 Harry Hines Blvd, Dallas, Texas 75390, USA
ISSN:2041-1723
2041-1723
DOI:10.1038/ncomms16068