Flat clathrin lattices are dynamic actin-controlled hubs for clathrin-mediated endocytosis and signalling of specific receptors

Clathrin lattices at the plasma membrane coat both invaginated and flat regions forming clathrin-coated pits and clathrin plaques, respectively. The function and regulation of clathrin-coated pits in endocytosis are well understood but clathrin plaques remain enigmatic nanodomains. Here we use super...

Celý popis

Uloženo v:

Podrobná bibliografie
Vydáno v:	Nature communications Ročník 8; číslo 1; s. 16068 - 14
Hlavní autoři:	Leyton-Puig, Daniela, Isogai, Tadamoto, Argenzio, Elisabetta, van den Broek, Bram, Klarenbeek, Jeffrey, Janssen, Hans, Jalink, Kees, Innocenti, Metello
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	London Nature Publishing Group UK 13.07.2017 Nature Publishing Group Nature Portfolio
Témata:	13 13/106 13/89 13/95 14 14/19 14/28 14/63 631/80/128/1276 631/80/313/1461 631/80/86/2368 96 96/10 96/34 96/35 Actin Actin Cytoskeleton - metabolism Actin-Related Protein 2-3 Complex - metabolism AKT protein Cell adhesion Cell adhesion & migration Cell migration Clathrin Clathrin - physiology Clathrin-Coated Vesicles - metabolism Coated pits Coating Control Dismantling Endocytosis Genetics HeLa Cells Hubs Humanities and Social Sciences Humans Lattices Machinery and equipment multidisciplinary Pits Plaques Polymerization Receptors Receptors, Lysophosphatidic Acid - metabolism Science Science (multidisciplinary) Signaling Wiskott-Aldrich Syndrome Protein, Neuronal - metabolism
ISSN:	2041-1723, 2041-1723
On-line přístup:	Získat plný text
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!

Popis
Shrnutí:	Clathrin lattices at the plasma membrane coat both invaginated and flat regions forming clathrin-coated pits and clathrin plaques, respectively. The function and regulation of clathrin-coated pits in endocytosis are well understood but clathrin plaques remain enigmatic nanodomains. Here we use super-resolution microscopy, molecular genetics and cell biology to show that clathrin plaques contain the machinery for clathrin-mediated endocytosis and cell adhesion, and associate with both clathrin-coated pits and filamentous actin. We also find that actin polymerization promoted by N-WASP through the Arp2/3 complex is crucial for the regulation of plaques but not pits. Clathrin plaques oppose cell migration and undergo actin- and N-WASP-dependent disassembly upon activation of LPA receptor 1, but not EGF receptor. Most importantly, plaque disassembly correlates with the endocytosis of LPA receptor 1 and down-modulation of AKT activity. Thus, clathrin plaques serve as dynamic actin-controlled hubs for clathrin-mediated endocytosis and signalling that exhibit receptor specificity. Clathrin lattices coat flat membrane regions, called plaques, whose regulation and function are poorly understood. Here the authors find that plaques are regulated by actin dynamics and contain both the endocytic and the cell adhesion machineries, and are involved in endocytosis of specific cargos, and cell migration.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 These authors contributed equally to this work. Present address: Department of Cell Biology; Lyda Hill Department of Bioinformatics, University of Texas Southwestern Medical Center, 6000 Harry Hines Blvd, Dallas, Texas 75390, USA
ISSN:	2041-1723 2041-1723
DOI:	10.1038/ncomms16068