Labeling and tracking of immune cells in ex vivo human skin

Human skin harbors various immune cells that are crucial for the control of injury and infection. However, the current understanding of immune cell function within viable human skin tissue is limited. We developed an ex vivo imaging approach in which fresh skin biopsies are mounted and then labeled...

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Bibliographic Details
Published in:	Nature protocols Vol. 16; no. 2; pp. 791 - 811
Main Authors:	Dijkgraaf, Feline E., Toebes, Mireille, Hoogenboezem, Mark, Mertz, Marjolijn, Vredevoogd, David W., Matos, Tiago R., Teunissen, Marcel B. M., Luiten, Rosalie M., Schumacher, Ton N.
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 01.02.2021 Nature Publishing Group
Subjects:	631/1647/245/2186 631/1647/328/1978 631/1647/328/2057 631/1647/767/2202 631/250/1619/554/1834 Analytical Chemistry Animals Antibodies Biological Techniques Biomedical and Life Sciences Biopsy Biopsy - methods CD4-Positive T-Lymphocytes - immunology CD8 antigen CD8-Positive T-Lymphocytes - immunology Cell culture Cell Culture Techniques - methods Cell interaction Cell research Cell surface Computational Biology/Bioinformatics Health aspects Humans Identification and classification Imaging Immune system Immunological memory Labeling Life Sciences Lymphocytes Lymphocytes T Markers Memory cells Methods Mice Microarrays Microscope and microscopy Nanobodies Nanotechnology Organic Chemistry Physiological aspects Protocol Reagents Skin Skin - cytology Skin - immunology Skin - pathology Skin diseases Staining and Labeling - methods Surface markers T cells Tracking Netherlands
ISSN:	1754-2189, 1750-2799, 1750-2799
Online Access:	Get full text
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