A portable pencil-like immunosensor for point-of-care testing of inflammatory biomarkers

Portable devices for immunoassays are in high demand for point-of-care testing (POCT) of biomarkers. Here, we report a robust portable pencil-like immunosensor (PPS) platform for the determination of three inflammatory biomarkers including interleukin-6 (IL-6), procalcitonin (PCT), and C-reactive pr...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry Vol. 412; no. 13; pp. 3231 - 3239
Main Authors: Nie, Rongbin, Huang, Jingwen, Xu, Xuexue, Yang, Li
Format: Journal Article
Language:English
Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.05.2020
Springer
Springer Nature B.V
Subjects:
ambient temperature
Analytical Chemistry
Biochemistry
Biological markers
Biomarkers
Biomarkers - metabolism
Biosensing Techniques
blood serum
C-reactive protein
C-Reactive Protein - metabolism
Characterization and Evaluation of Materials
Chemiluminescence
Chemistry
Chemistry and Materials Science
detection limit
Food Science
Humans
Immunoassay
Immunoassays
Immunosensors
Inflammation
Inflammation - metabolism
Interleukin 6
Interleukins
Laboratory Medicine
Limit of Detection
Monitoring/Environmental Analysis
photons
Point of care testing
Point-of-Care Systems
Portable equipment
Procalcitonin
Reagents
Reproducibility of Results
Research Paper
Room temperature
Sensors
storage temperature
storage time
China
Point-of-care testing
Pencil-like immunosensor
Procalcitonin
C-Reactive protein
Interleukin-6
Optical fiber
ISSN:1618-2642, 1618-2650, 1618-2650
Online Access:Get full text
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Summary:Portable devices for immunoassays are in high demand for point-of-care testing (POCT) of biomarkers. Here, we report a robust portable pencil-like immunosensor (PPS) platform for the determination of three inflammatory biomarkers including interleukin-6 (IL-6), procalcitonin (PCT), and C-reactive protein (CRP) in human serum samples. The PPS platform is composed of a unique pencil-like optical-fiber-based sensor, a reagent strip consisting of a series of pencil-cap-like wells, and a battery-powered photon counting detector for recording chemiluminescence. The PPS probe moves from well to well with a plug-into/out approach and goes through the immunoassay steps. Each fiber probe in the PPS platform can be sequentially used in up to 10 assays by simply propelling the intact probe out of the pencil body. The PPS platform is well-integrated into a portable suitcase-like device (32 cm × 23 cm × 11 cm) and is only 3 kg in weight. The sensor has good repeatability and can maintain 90% response after 14 days of storage at room temperature, showing its ability for assays in the field. The good linear relationship and efficient dynamic range with a limit-of-detection (LOD) of 1.05 pg/mL for IL-6, 10.64 pg/mL for PCT, and 29.40 ng/mL for CRP are obtained. The assay results are compared with clinical methods, and the findings confirm the high accuracy and precision of the proposed method. The proposed PPS platform is versatile and operable with minimal instruments and technical skills and simplifies the process of immune analysis, thus has great prospects for POCT of biomarkers. Graphical abstract
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ISSN:1618-2642
1618-2650
1618-2650
DOI:10.1007/s00216-020-02582-z