Characteristics of RNA Stabilizer RNApro for Peripheral Blood Collection

Peripheral blood is the most practical tissue for human immune system gene expression profiling because it is easily accessible, whereas the site of primary infection in certain diseases may not be easily accessible. Due to the ex vivo instability of RNA transcripts, a key challenge in the gene expr...

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Bibliographic Details
Published in:Diagnostics (Basel) Vol. 14; no. 10; p. 971
Main Authors: Gambarino, Stefano, Galliano, Ilaria, Clemente, Anna, Calvi, Cristina, Montanari, Paola, Pau, Anna, Dini, Maddalena, Bergallo, Massimiliano
Format: Journal Article
Language:English
Published: Switzerland MDPI AG 01.05.2024
Subjects:
Anticoagulants
Biomarkers
Blood collection and preservation
Chemical properties
Enzymes
GAPDH
Gene expression
guanidinium thiocyanate
Health aspects
Laboratories
Methods
MicroRNAs
Oxidation
Preservatives
RNA
RNA extraction
RNA stabilizers
RNApro
Stabilizing agents
Temperature
Italy
United States > US
RNA extraction
GAPDH
RNA stabilizers
guanidinium thiocyanate
RNApro
ISSN:2075-4418, 2075-4418
Online Access:Get full text
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Summary:Peripheral blood is the most practical tissue for human immune system gene expression profiling because it is easily accessible, whereas the site of primary infection in certain diseases may not be easily accessible. Due to the ex vivo instability of RNA transcripts, a key challenge in the gene expression analysis of blood samples is the rapid sample handling and stabilization of the mRNA by adding an RNA preservative (PAXgeneTM Blood RNA Tubes, TempusTM Blood RNA tubes, RNAlater Stabilization Reagent, RNAgard® Blood Tubes). BioMole (Turin, Italy) has developed a novel blood stabilizer, called RNApro, in which RNA is stabilized during phlebotomy and sample storage. In this study, RNApro performance intended as RNA yield, integrity, and stability was evaluated. Our results show that blood samples stored at −80 °C and re-extracted after 7 years show no differences in terms of quantity, quality, and amplificability. The samples in the RNAlater stabilization solution can be stored at room temperature for up to one week or at 4 °C for up to one month. Similar results can also be observed for PAXgene tubes, Tempus tubes, and RNAgard tubes. In agreement with these data, the RNApro stabilization solution preserves the RNA from degradation for up to 1 month at 4 °C and 1 week at room temperature. RNApro can be stored indifferently at −80, −20, 4 °C, or room temperature for up to 2 months after, and then could be stored at −80 °C for up to seven years. In summary, our study is the first to analyze the performance of an RNA stabilizer called RNApro. We can conclude that several studies have shown significant differences in gene expression analysis when the sample was preserved in different RNA stabilizers. Therefore, it is desirable to standardize the method of nucleic acid conservation when comparing data from transcriptomic analyses.
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ISSN:2075-4418
2075-4418
DOI:10.3390/diagnostics14100971