Particle packed columns and monolithic columns in high-performance liquid chromatography-comparison and critical appraisal

The review highlights the fundamentals and the most prominent achievements in the field of high-performance liquid chromatography (HPLC) column development over a period of nearly 50 years. After a short introduction on the structure and function of HPLC columns, the first part treats the major step...

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Veröffentlicht in:Journal of Chromatography A Jg. 1184; H. 1; S. 393 - 415
Hauptverfasser: Unger, Klaus K., Skudas, Romas, Schulte, Michael M.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Amsterdam Elsevier B.V 14.03.2008
Amsterdam; New York: Elsevier
Elsevier
Schlagworte:
Analytical chemistry
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, High Pressure Liquid - instrumentation
Exact sciences and technology
Fractionation, Field Flow
Fundamental aspects and recent developments
High-performance liquid chromatography
manufacturing
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Miniaturization
Monolithic columns
Other chromatographic methods
Particle packed columns
Particle Size
Silicon Dioxide - chemistry
simulation models
Particle packed columns
High-performance liquid chromatography
Monolithic columns
Fundamental aspects and recent developments
Particle size
Pore structure
Monolithic column
HPLC chromatography
Review
Particle size distribution
Preparation
Performance
Packed column
Comparative study
ISSN:0021-9673
Online-Zugang:Volltext
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Abstract The review highlights the fundamentals and the most prominent achievements in the field of high-performance liquid chromatography (HPLC) column development over a period of nearly 50 years. After a short introduction on the structure and function of HPLC columns, the first part treats the major steps and processes in the manufacture of a particle packed column: synthesis and control of particle morphology, sizing and size analysis, packing procedures and performance characterization. The next section is devoted to three subjects, which reflect the recent development and the main future directions of packed columns: minimum particle size of packing, totally porous vs. core/shell particles and column miniaturization. In the last section an analysis is given on an alternative to packed columns—monolithic columns, which have gained considerable attraction. The challenges are: improved packing design based on modeling and simulation for targeted applications, and enhanced robustness and reproducibility of monolithic columns. In the field of miniaturization, particularly in chip-based nano-LC systems, monoliths offer a great potential for the separation of complex mixtures e.g. in life science.
AbstractList The review highlights the fundamentals and the most prominent achievements in the field of high-performance liquid chromatography (HPLC) column development over a period of nearly 50 years. After a short introduction on the structure and function of HPLC columns, the first part treats the major steps and processes in the manufacture of a particle packed column: synthesis and control of particle morphology, sizing and size analysis, packing procedures and performance characterization. The next section is devoted to three subjects, which reflect the recent development and the main future directions of packed columns: minimum particle size of packing, totally porous vs. core/shell particles and column miniaturization. In the last section an analysis is given on an alternative to packed columns--monolithic columns, which have gained considerable attraction. The challenges are: improved packing design based on modeling and simulation for targeted applications, and enhanced robustness and reproducibility of monolithic columns. In the field of miniaturization, particularly in chip-based nano-LC systems, monoliths offer a great potential for the separation of complex mixtures e.g. in life science.
The review highlights the fundamentals and the most prominent achievements in the field of high-performance liquid chromatography (HPLC) column development over a period of nearly 50 years. After a short introduction on the structure and function of HPLC columns, the first part treats the major steps and processes in the manufacture of a particle packed column: synthesis and control of particle morphology, sizing and size analysis, packing procedures and performance characterization. The next section is devoted to three subjects, which reflect the recent development and the main future directions of packed columns: minimum particle size of packing, totally porous vs. core/shell particles and column miniaturization. In the last section an analysis is given on an alternative to packed columns-monolithic columns, which have gained considerable attraction. The challenges are: improved packing design based on modeling and simulation for targeted applications, and enhanced robustness and reproducibility of monolithic columns. In the field of miniaturization, particularly in chip-based nano-LC systems, monoliths offer a great potential for the separation of complex mixtures e.g. in life science.The review highlights the fundamentals and the most prominent achievements in the field of high-performance liquid chromatography (HPLC) column development over a period of nearly 50 years. After a short introduction on the structure and function of HPLC columns, the first part treats the major steps and processes in the manufacture of a particle packed column: synthesis and control of particle morphology, sizing and size analysis, packing procedures and performance characterization. The next section is devoted to three subjects, which reflect the recent development and the main future directions of packed columns: minimum particle size of packing, totally porous vs. core/shell particles and column miniaturization. In the last section an analysis is given on an alternative to packed columns-monolithic columns, which have gained considerable attraction. The challenges are: improved packing design based on modeling and simulation for targeted applications, and enhanced robustness and reproducibility of monolithic columns. In the field of miniaturization, particularly in chip-based nano-LC systems, monoliths offer a great potential for the separation of complex mixtures e.g. in life science.
Author Unger, Klaus K.
Skudas, Romas
Schulte, Michael M.
Author_xml – sequence: 1
  givenname: Klaus K.
  surname: Unger
  fullname: Unger, Klaus K.
  email: k.k.unger@web.de
  organization: Institut für Anorganische Chemie und Analytische Chemie, Johannes Gutenberg-Universität-Mainz, Duesbergweg 10-14, D-55099 Mainz, Germany
– sequence: 2
  givenname: Romas
  surname: Skudas
  fullname: Skudas, Romas
  organization: Institut für Anorganische Chemie und Analytische Chemie, Johannes Gutenberg-Universität-Mainz, Duesbergweg 10-14, D-55099 Mainz, Germany
– sequence: 3
  givenname: Michael M.
  surname: Schulte
  fullname: Schulte, Michael M.
  organization: Performance & Life Science Chemicals R&D, Merck KGaA, Frankfurter Land Str. 250, 64293 Darmstadt, Germany
BackLink http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20176049$$DView record in Pascal Francis
https://www.ncbi.nlm.nih.gov/pubmed/18177658$$D View this record in MEDLINE/PubMed
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Issue 1
Keywords Particle packed columns
High-performance liquid chromatography
Monolithic columns
Fundamental aspects and recent developments
Particle size
Pore structure
Monolithic column
HPLC chromatography
Review
Particle size distribution
Preparation
Performance
Packed column
Comparative study
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Snippet The review highlights the fundamentals and the most prominent achievements in the field of high-performance liquid chromatography (HPLC) column development...
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SubjectTerms Analytical chemistry
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, High Pressure Liquid - instrumentation
Exact sciences and technology
Fractionation, Field Flow
Fundamental aspects and recent developments
High-performance liquid chromatography
manufacturing
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Miniaturization
Monolithic columns
Other chromatographic methods
Particle packed columns
Particle Size
Silicon Dioxide - chemistry
simulation models
Title Particle packed columns and monolithic columns in high-performance liquid chromatography-comparison and critical appraisal
URI https://dx.doi.org/10.1016/j.chroma.2007.11.118
https://www.ncbi.nlm.nih.gov/pubmed/18177658
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Volume 1184
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