Bile analysis using high-throughput FTIR spectroscopy for the diagnosis of malignant biliary strictures: a pilot study in 57 patients

This study aimed at determining whether FTIR spectroscopy is able to distinguish bile samples from patients with and without malignant biliary strictures. Bile samples were collected in 19 patients with malignant biliary strictures and 38 with benign biliary diseases during endoscopic procedures. FT...

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Veröffentlicht in:Journal of biophotonics Jg. 7; H. 3-4; S. 241 - 253
Hauptverfasser: Untereiner, Valérie, Dhruvananda Sockalingum, Ganesh, Garnotel, Roselyne, Gobinet, Cyril, Ramaholimihaso, Fidy, Ehrhard, Florent, Diebold, Marie-Danièle, Thiéfin, Gérard
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Berlin WILEY-VCH Verlag 01.04.2014
WILEY‐VCH Verlag
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Schlagworte:
Adult
Aged
bile
Bile - chemistry
biliary tract cancer
Biliary Tract Diseases - diagnosis
biomarkers
Chemometrics
Classifiers
Constriction, Pathologic - diagnosis
Endoscopy - methods
Female
Fourier transform infrared spectroscopy
Fourier transforms
Humans
Infrared spectroscopy
Lipids - blood
Male
Middle Aged
Patients
Pilot Projects
Pilots
Principal Component Analysis
Principal components analysis
Reproducibility of Results
Sensitivity and Specificity
Signal Processing, Computer-Assisted
Spectra
Spectroscopy
Spectroscopy, Fourier Transform Infrared - methods
Support Vector Machine
biliary tract cancer
Fourier transform infrared spectroscopy
support vector machine
biomarkers
bile
ISSN:1864-063X, 1864-0648, 1864-0648
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Zusammenfassung:This study aimed at determining whether FTIR spectroscopy is able to distinguish bile samples from patients with and without malignant biliary strictures. Bile samples were collected in 19 patients with malignant biliary strictures and 38 with benign biliary diseases during endoscopic procedures. FTIR spectra were acquired on dried drops of whole bile, aqueous and organic phases obtained after lipid extraction. Data were analyzed by principal component analysis and by the support vector machine classification using a leave‐n‐out cross validation procedure. This was applied to the whole set of spectra and the mean and median spectra of each patient. By leaving one patient out, the classifier allowed discriminating patients with and without malignant biliary strictures with a sensitivity between 82% and 95% and a specificity between 85% and 100%. Using a randomized leave‐n ‐out cross‐validation with n = 2, 5 and 10 patients, the sensitivity decreased slightly by about 5 to 10% while the specificity remained stable, suggesting the robustness of the classifier. FTIR spectroscopy combined with chemometrics therefore shows potential to differentiate bile from patients with and without malignant biliary strictures. Although promising, the results of this pilot study cannot be generalized and needs to be confirmed in a larger population. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Bibliographie:Reims University Hospital (Appel d'Offre Local 2011)
ArticleID:JBIO201300166
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ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1864-063X
1864-0648
1864-0648
DOI:10.1002/jbio.201300166